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2 protocols using goat anti mouse igg horseradish peroxidase hrp antibody sc 2005

1

Amyloid-Beta Immunodetection Assay

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We purchased the following antibodies and reagents from the respective manufacturers listed below. A mouse monoclonal antibody to Aβ (6E10) (SIG-39300, Covance, CA, USA); a rabbit polyclonal antibody to APP, C-terminal (A8717, Sigma-Aldrich, MO, USA); a β-actin rabbit antibody (#4970, Cell signaling, MA, USA); a goat anti-mouse IgG-horseradish peroxidase (HRP) antibody (sc-2005, Santa Cruz Biotechnology, CA, USA); a goat anti-rabbit IgG-HRP antibody (sc-2054, Santa Cruz Biotechnology); Alexa Fluor 488 Goat anti-rabbit IgG (H + L) (A11088, Life Technologies, CA, USA); Human β Amyloid (1–40) ELISA kit (292-62301, Wako, Osaka, Japan); Human β Amyloid (1–42) ELISA kit (298-62401, Wako); Protein G-Agarose (11719416001, Roche, Basel, Switzerland); hygromycin B (400052, Calbiochem, CA, USA); SuperSignal West Femto Maximum Sensitivity Substrate (#34095, Thermo Fisher Scientific, Rockford, USA); SuperSignal ELISA Femto Maximum Sensitivity Substrate (#37075, Thermo Fisher Scientific); Ham’s F-12 medium (17458-65, Nacalai Tesque, Kyoto, Japan); Penicillin-Streptomycin (15140-122, Gibco, CA, USA); and fetal bovine serum (SH30910.03, Hyclone, MA, USA).
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2

Amyloid Precursor Protein Analysis

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Nicotine, 3-buten-2-one (methyl vinyl ketone; MVK), and a rabbit polyclonal antibody to the APP C-terminal (A8717) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A rabbit polyclonal antibody to BACE1 was from Abcam (Cambridge, UK). A rabbit polyclonal antibody to phospho APP (Thr668, #3823) and a β-actin antibody (#4970) were from Cell Signaling Technology (Danvers, MA, USA). A goat anti-mouse IgG-horseradish peroxidase (HRP) antibody (sc-2005) and a goat anti-rabbit IgG-HRP antibody (sc-2054) were from Santa Cruz Biotechnology (Dallas, TX, USA). Hygromycin B (400052) was from Calbiochem (La Jolla, CA, USA). SuperSignal West Femto Maximum Sensitivity Substrate (#34095) and SuperSignal ELISA Femto Maximum Sensitivity Substrate (#37075) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Ham's F-12 medium (17458-65) was from Nacalai Tesque (Kyoto, Japan) and penicillin-streptomycin (15140-122) from Gibco (Palo Alto, CA, USA). Fetal bovine serum (SH30910.03) was obtained from Hyclone (Chicago, IL, USA).
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