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3 protocols using jacobine

1

Pyrrolizidine Alkaloids Extraction and Analysis

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PAs such
as senecionine, senecionine N-oxide,
retrorsine, retrorsine N-oxide, seneciphylline, seneciphylline N-oxide, jacoline, jacoline N-oxide, jaconine,
merenskine N-oxide (isomer of jaconine N-oxide), jacobine, jacobine N-oxide, erucifoline,
and erucifoline N-oxide were purchased from Phytoplan
(Heidelberg, Germany) or in the case of riddelliine and riddelliine N-oxide from Oskar Tropitzsch (Marktredwitz, Germany). Methanol
and water (both LC–MS grade) were purchased from Merck KGA
(Darmstadt, Germany). Ingredients of the in vitro incubation buffer
were obtained from Carl Roth (Karlsruhe, Germany). All chemicals obtained
were of the highest purity that was commercially available.
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2

Quantification of Pyrrolizidine Alkaloids

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Analytical standards were obtained from different suppliers as follows: echimidine (purity 97%), echimidine N-oxide (purity 97%), heliotrine (purity 91%), heliotrine N-oxide (purity 91%), lycopsamine (purity 80%) and lycopsamine N-oxide (purity 80%) from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany); senecionine (purity 99%), senecionine N-oxide (purity 99%), seneciphylline (purity 94%) and seneciphylline N-oxide (purity 94%) from Carl Roth & Co. KG (Karlsruhe, Germany); indicine-N-oxide (purity 99%), intermedine (purity 99%), jacobine (purity 98%), jacobine N-oxide (purity 98%), retrorsine (purity 90%), retrorsine N-oxide (purity 96.0%) and senkirkine (purity 98%) from Phytoplan (Heidelberg, Germany); caffeine (purity 98%), used as an internal standard, was from Sigma-Aldrich (Steinheim, Germany).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
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3

Quantification of Pyrrolizidine Alkaloids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical standards were obtained from different suppliers as follows: echimidine (purity 97%), echimidine N-oxide (purity 97%), heliotrine (purity 91%), heliotrine N-oxide (purity 91%), lycopsamine (purity 80%) and lycopsamine N-oxide (purity 80%) from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany); senecionine (purity 99%), senecionine N-oxide (purity 99%), seneciphylline (purity 94%) and seneciphylline N-oxide (purity 94%) from Carl Roth & Co. KG (Karlsruhe, Germany); indicine-N-oxide (purity 99%), intermedine (purity 99%), jacobine (purity 98%), jacobine N-oxide (purity 98%), retrorsine (purity 90%), retrorsine N-oxide (purity 96.0%) and senkirkine (purity 98%) from Phytoplan (Heidelberg, Germany); caffeine (purity 98%), used as an internal standard, was from Sigma-Aldrich (Steinheim, Germany).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
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