Caldesmon

Manufactured by Santa Cruz Biotechnology

Caldesmon is a protein that regulates the interaction between actin and myosin in smooth muscle cells. It plays a role in the contractile function of these cells. Caldesmon is often used in research applications to study the mechanisms of smooth muscle contraction.

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Lab products found in correlation

Notch3, by Cell Signaling Technology (1 mentions) Notch2, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Nacalai Tesque (1 mentions) Peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Calponin, by Merck Group (1 mentions) Notch1, by Abcam (1 mentions) Smoothelin, by Abcam (1 mentions) Calponin, by Santa Cruz Biotechnology (1 mentions) 0.2 μm pvdf membrane, by Bio-Rad (1 mentions) Odyssey, by LI COR (1 mentions) Tris hcl gel, by Bio-Rad (1 mentions) Nonidet p 40, by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using caldesmon

Western Blot Analysis of Smooth Muscle Markers

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MCs were lysed in lysis solution (1% SDS, 4 M urea, 1 mM EDTA, 150 mM NaCl, 50 mM Tris, protease inhibitor cocktail (nacalai tesque), pH 8.0) and ultrasonic treatment applied. For western blotting the following antibodies were used: SMMHC2 and GAPDH from abcam, Notch1, Notch2, Notch3 and HES1 from Cell Signaling Technology, Calponin from Millipore, Caldesmon from Santa Cruz Biotechnology, peroxidase-conjugated secondary antibodies from Jackson ImmunoResearch.

Yamamoto Y., Kojima K., Taura D., Sone M., Washida K., Egawa N., Kondo T., Minakawa E.N., Tsukita K., Enami T., Tomimoto H., Mizuno T., Kalaria R.N., Inagaki N., Takahashi R., Harada-Shiba M., Ihara M, & Inoue H. (2020). Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease. Molecular Brain, 13, 38.

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Protein Expression Analysis of Smooth Muscle Cells

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Tissues were lysed with RIPA buffer (100mM Tris (pH 7.4) (Sigma Aldrich), 4M Urea (Sigma Aldrich), 5mM EDTA (Sigma Aldrich), 0.5% SDS (Sigma Aldrich), 0.5% Nonidet P-40 (Sigma Aldrich), and protease inhibitor cocktail (Complete mini, Roche)) 36h after seeding. 15μg of total protein for each sample was run through a 4–15% Tris-HCL gel (Bio-Rad, Hercules, CA) at 120V for 2h. Then the samples were transferred to 0.2μm PVDF membrane (Bio-Rad). The following primary antibodies were incubated overnight at 4°C: smoothelin (Abcam), Calponin (Santa Cruz Biotechnology, Dallas, TX), Caldesmon (Santa Cruz Biotechnology), and β-actin (Santa Cruz Biotechnology). Protein bands were visualized after 1h incubation in LICOR secondary antibodies on the LICOR Odyssey (LICOR, Lincoln, NE). Image Studio Lite Version 3.1 was used to quantify protein amounts via densitometry. Each protein band value was normalized to its respective β-actin loading control and then normalized to the 10kPa substrate construct.

Steucke K.E., Tracy P.V., Hald E.S., Hall J.L, & Alford P.W. (2015). Vascular smooth muscle cell functional contractility depends on extracellular mechanical properties. Journal of biomechanics, 48(12), 3044-3051.

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