Depmpo

3-aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na₂S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), S-nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-d-1-thiogalactopyranoside (IPTG) were obtained from Sigma-Aldrich. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red were obtained from Thermo Fisher Scientific. DEPMPO was obtained from Enzo. H₂O₂ was obtained from Roth. GSH and 5-aminolevulinic acid hydrochloride were obtained from Merck. Sodium disulfide (Na₂S₂), sodium tetrasulfide (Na₂S₄) and 3′,6′-di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS³⁴SSG and GSSSSG was performed as described previously⁴³ . CSSSC was synthesized as described below.

The compounds under investigation were synthesized and subsequently subjected to recrystallization using the procedure outlined in the previously described publication [17 (link)]. The chemicals H₂O₂, FeSO₄, phosphate buffer, and DMSO were obtained from Sigma-Aldrich (St. Louis, MO, USA) as reagents of analytical grade and were utilized without any modifications [21 (link),22 (link)]. The spin-trap DEPMPO was procured from Enzo Life Sciences and subsequently purified using the methodology outlined by Jackson [23 (link)].