Colorectal samples were fixed in 4% paraformaldehyde. Tissues were embedded in paraffin and cut into 4-μm sections. The sections were submerged in xylene at 40ºC for 30 min for deparaffinization. Deparaffinized tissues were dehydrated in a gradient of alcohol solutions (100%, 95%, 80% and 75%) for 1 min in each. The sections were washed in distilled water for 1 min and then stained with haematoxylin and eosin (HE). After washing three times in PBS for 3 min, the deparaffinized slides were submerged in 120℃ distilled water containing citrate (1: 100) or EDTA (1: 50) for 2 min and incubated for 20 min at room temperature for antigen retrieval. Endogenous peroxidase activity was blocked with 1.0% H2O2 for 10 min. The sections were incubated with mouse anti-human CD68 (KPI, ab955, Abcam), rabbit anti-human HLA-DR (EPR3692, ab92511, Abcam) and rabbit anti-human CD163 (EPR19518, ab182422, Abcam) monoclonal antibodies at 4°C overnight, followed by incubation with a secondary antibody (Max VisionTM HRP kit-5020, MXB, China). The HRP-conjugated secondary antibody was visualized by development with diaminobenzidine (DAB; DAB-0031/1031, MXB, China). All sections were counterstained with haematoxylin.
Dab 0031 1031
Manufactured by Beijing Strong Biotechnologies
Sourced in China
The DAB-0031/1031 is a laboratory equipment product. It is designed for laboratory use and serves a core function. No further details can be provided in an unbiased and factual manner without potential extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dab 0031 1031
1
Immunohistochemical Analysis of Colorectal Samples
2
Quantifying HLA-DR Expression in HNSCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tissues were dewaxed and stained with hematoxylin and eosin (HE). HLA-DR protein expression was determined by immunostaining using the rabbit anti-human HLA-DR (1: 500 dilution; EPR3692, ab92511, Abcam). The HRP-conjugated secondary antibody was visualized by development with diaminobenzidine (DAB; DAB-0031/1031, MXB, China).
Image Pro Plus 6.0 software was used to analyze the tissue photos. Mean optical density (OD) (31) (link) was used to determine the difference of HLA-DR protein expression between HNSCC tissues and adjacent normal tissues.
Hazard ratio (HR) and 95% confidence interval (CI) obtained from univariate and multivariate Cox regression analysis (32, (link)33) (link) The accuracy of the risk score for survival prediction was evaluated by Kaplan-Meier survival curve and ROC curve (34) (link), and the acreage under the ROC curve (AUC) was calculated. Principal component analysis (PCA).
The Seurat package, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The above analysis was performed by R software (version 3.6.2). Gene set enrichment analysis (GSEA) (http://software.broadinstitute.org/gsea/index.jsp) was used for functional annotation of high-low risk groups, and a P value <0.05 was considered to be
Image Pro Plus 6.0 software was used to analyze the tissue photos. Mean optical density (OD) (31) (link) was used to determine the difference of HLA-DR protein expression between HNSCC tissues and adjacent normal tissues.
Hazard ratio (HR) and 95% confidence interval (CI) obtained from univariate and multivariate Cox regression analysis (32, (link)33) (link) The accuracy of the risk score for survival prediction was evaluated by Kaplan-Meier survival curve and ROC curve (34) (link), and the acreage under the ROC curve (AUC) was calculated. Principal component analysis (PCA).
The Seurat package, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The above analysis was performed by R software (version 3.6.2). Gene set enrichment analysis (GSEA) (http://software.broadinstitute.org/gsea/index.jsp) was used for functional annotation of high-low risk groups, and a P value <0.05 was considered to be
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!