For the detection of PRRSV proteins, monoclonal antibody (mAb) 13E2 directed against PRRSV N, and mAb VII2D directed against amino acids 73–84 of PRRSV GP3 were kindly provided by Hans Nauwynck (University Ghent, Belgium). A polyclonal rabbit serum against PRRSV GP4 was obtained from Luis Enjuanes (Centro Nacional de Biotecnología, Madrid, Spain). This serum was generated at BioGenes (Germany) with purified recombinant GP4 from PRRSV Olot/91 expressed with the baculovirus system. The mAb 11E10C7 directed against PRRSV M, and the mAb 3AH9 against PRRSV GP5 were a gift from INGENASA (Madrid, Spain). The rabbit anti-Myc antiserum C3956, the mouse anti-Flag M2 antibody and the anti-α-tubulin mAb were purchased from Sigma-Aldrich. The mouse anti-HA antibody 12CA5 was from Roche. The secondary antibodies goat anti-mouse IgG Alexa 546 and goat anti-rabbit IgG Alexa 546 were from Molecular Probes. The anti-swine IgG antibody conjugated with rhodamine was purchased from Rockland. The polyclonal rabbit anti-mouse IgG coupled with horseradish peroxidase was from DAKO.
Mouse anti ha antibody 12ca5
Manufactured by Roche
The Mouse anti-HA antibody 12CA5 is a monoclonal antibody specifically designed to recognize the hemagglutinin (HA) epitope tag. It is a widely used tool in molecular biology and biochemistry research for the detection and immunopurification of HA-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg alexa 546, by Thermo Fisher Scientific (1 mentions)
Anti α tubulin mab, by Merck Group (1 mentions)
Goat anti mouse igg alexa546, by Thermo Fisher Scientific (1 mentions)
Mouse anti flag m2 antibody, by Merck Group (1 mentions)
Mouse anti v5 antibody, by Thermo Fisher Scientific (1 mentions)
Mg132, by Merck Group (1 mentions)
2 protocols using mouse anti ha antibody 12ca5
1
Detecting PRRSV Proteins: Monoclonal Antibody Assay
2
Ubiquitination analysis of HORMAD1 by FBXO family proteins
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FLAG-FBXO47, MYC-FLAG-FBXW8, MYC-FLAG-FBXW17, MYC-FLAG-FBXO28, V5-HORMAD1 and HA-ubiquitin expression plasmids were co-transfected into HEK293T cells (Supplementary Table S3 ). After 24 h, 10 μm MG132 (M8699, Sigma) was added and incubated for 4 h to inhibit proteasome. Transfected cells were lysed with the RIPA buffer supplemented with 1 mM PMSF. Cell lysates were incubated with mouse anti-V5 antibody (R960-25, Invitrogen) at 4°C overnight and then incubated with Dynabeads G at 4°C for 3 h. The precipitants were washed three times with the RIPA buffer and followed by western blot analysis with mouse anti-HA antibody (12CA5, Roche) and rabbit anti-HORMAD1 antibody (13917-1-AP, Proteintech).
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