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Acrylamide and bisacrylamide

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Acrylamide and bisacrylamide are organic compounds commonly used as polymerizing agents in electrophoresis techniques. Acrylamide forms linear polymers, while bisacrylamide forms cross-linked polymers. These compounds are essential components in the preparation of polyacrylamide gels, which are used to separate and analyze biomolecules such as proteins and nucleic acids based on their size and charge.

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Lab products found in correlation

Ecolite, by MP Biomedicals (1 mentions) Digitonin, by Serva Electrophoresis (1 mentions) Coomassie blue g 250, by Serva Electrophoresis (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) α 32p atp, by Cytiva (1 mentions) Protran, by Cytiva (1 mentions)

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Acrylamide (6 citations)

3 protocols using acrylamide and bisacrylamide

1

Antibody Characterization and Reagent Sources

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[α-32P]ATP was purchased from Izotop (Budapest, Hungary), [3H]cAMP from ARC (St. Louis, MO, USA), and scintillation cocktail EcoLite from MP Biomedicals (Santa Ana, CA, USA). Acrylamide and bisacrylamide were from SERVA (Heidelberg, Germany), and aluminum oxide 90 (neutral, activity I) was from Merck (Darmstadt, Germany). All other chemicals were from Sigma (St. Louis, MO, USA) and they were of the highest purity available. AC5 antibody was from Abcam (Cambridge, UK) and Gsα and actin antibodies were from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Preparation and characterization of Giα(1,2) antibody were described previously [17 (link)].
Hejnova L., Hahnova K., Kockova R., Svatunkova J., Sedmera D, & Novotny J. (2014). Adenylyl Cyclase Signaling in the Developing Chick Heart: The Deranging Effect of Antiarrhythmic Drugs. BioMed Research International, 2014, 463123.
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2

Yeast Mitochondrial Protein Analysis Protocol

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Imidazole, 6-aminohexanoic acid, digitonin (purity >50%) were obtained from Fluka. digitonin was used directly without recrystallization. Acrylamide and bisacrylamide (the commercial twice crystallized products), Coomassie blue G-250 (Serva Blue G) and digitonin used for experiments in Figs. 2 and 3 were purchased from Serva. All other chemicals were from Sigma. Yeast strains used in this study were wild type (WT) W303–1A (Mat a, leu2, trp1, ura3, his3, ade2) and the cox26 null mutant, Δcox26 (W303–1A, leu2, trp1, ura3, ade2, COX26::KAN). The Cox26 gene deletion yeast strain, Δcox26 was constructed in the W303–1A genetic background, as follows: Introducing the kanamycin resistance gene (KANr) into the COX26 (YDR119w-a) gene locus of wild type cells, resulted in the complete deletion of the open reading frame. The KANr gene was amplified from the plasmid pFA6a-KANMX6 [32 (link)].
Strains were grown in the following media: YPGal (yeast extract and bactopeptone containing 2% galactose), YPD (YP + 3% glucose), YPL (YP + 2% lactate), YPEG (YP + 2% ethanol, 3% glycerol), and YPGalLac (YP + 2% galactose and 0.5% lactate) at temperatures of 30 °C and 37 °C.
Strecker V., Kadeer Z., Heidler J., Cruciat C.M., Angerer H., Giese H., Pfeiffer K., Stuart R.A, & Wittig I. (2016). Supercomplex-associated Cox26 protein binds to cytochrome c oxidase. Biochimica et biophysica acta, 1863(7 Pt A), 1643-1652.
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3

Reagents and Materials Used

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TRIzol Reagent was from Invitrogen (Carlsbad, CA, USA), [α-32 P]ATP, [ 3 H]cAMP and [ 3 H]CGP 12177 were purchased from Amersham Biosciences (Buckinghamshire, UK) and scintillation cocktail CytoScint from ICN Biomedicals (Irvine, CA, USA). Acrylamide and bis-acrylamide were from SERVA (Heidelberg, Germany), aluminum oxide 90 (neutral, activity I) was from Merck (Darmstadt, Germany) and Protran nitrocellulose transfer membranes were from Schleicher & Schuell BioScience (Dassel, Germany). All other chemicals were from Sigma (St. Louis, MI, USA) and they were of the highest purity available.
Hahnova K., Kasparova D., Zurmanova J., Neckar J., Kolar F, & Novotny J. (2016). β-Adrenergic signaling in rat heart is similarly affected by continuous and intermittent normobaric hypoxia. General physiology and biophysics, 35(2).
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