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Mitoxpress assay

Manufactured by Agilent Technologies
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The MitoXpress assay is a fluorescence-based measurement tool designed to assess mitochondrial respiration and oxygen consumption in live cells. It provides a simple and robust method for monitoring real-time changes in oxygen levels within cellular samples.

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Lab products found in correlation

Ph xtra assay, by Agilent Technologies (2 mentions) Cytation 1 cell imaging multi mode reader, by Agilent Technologies (1 mentions) Cytation 1 imaging multimode reader, by Agilent Technologies (1 mentions)

Spelling variants of this product

MitoXpress Xtra Oxygen Consumption Assay (3 citations) MitoXpress® Xtra assay (2 citations)

2 protocols using mitoxpress assay

1

Evaluating mitochondrial function in activated PBMCs

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The extracellular acidification rate (ECAR) and OCR for mitochondrial oxidation were evaluated using the pH-Xtra assay (Agilent Technologies) and mitoXpress assay (Agilent Technologies), respectively. The manufacturer’s recommended procedures were followed. Human PBMCs, activated by plate-bound anti-CD3 and anti-CD28 antibodies for at least 3 d, were plated at 600,000 cells per well using fresh medium containing 4 mM glucose or 4 mM inosine. For ECAR, cells were incubated in the absence of CO2 for 2 h prior to the assay. Respiration buffer was customized by supplementing glucose or inosine. Both assays were read during 180 min at 37 °C in a Cytation 1 Cell Imaging Multi-Mode Reader (Biotek Instruments).
Wang T., Gnanaprakasam J.N., Chen X., Kang S., Xu X., Sun H., Liu L., Rodgers H., Miller E., Cassel T.A., Sun Q., Vicente-Muñoz S., Warmoes M.O., Lin P., Piedra-Quintero Z.L., Guerau-de-Arellano M., Cassady K.A., Zheng S.G., Yang J., Lane A.N., Song X., Fan T.W, & Wang R. (2020). Inosine is an alternative carbon source for CD8+-T-cell function under glucose restriction. Nature Metabolism, 2(7), 635-647.
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2

Mitochondrial Oxidation in Activated PBMCs

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The extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) for mitochondrial oxidation were evaluated using the pHXtra assay (Agilent Technologies) and mitoXpress assay (Agilent Technologies), respectively. Manufacturer’s recommended procedures were followed. Human PBMC cells, activated by plate-bound anti-CD3 and anti-CD28 antibodies for at least three days, were plated at 600,000 cells/well using fresh media containing 4 mM glucose or 4 mM Inosine. For ECAR, cells were incubated in the absence of CO2 for 2 hours prior to the assay. Respiration buffer was customized by supplementing glucose or inosine. Both assays were read during 180 minutes at 37 °C in a Cytation 1 imaging multi-mode reader (Biotek instruments).
Wang T., Gnanaprakasam J.N., Chen X., Kang S., Xu X., Sun H., Liu L., Rodgers H., Miller E., Cassel T.A., Sun Q., Vicente-Muñoz S., Warmoes M.O., Lin P., Piedra-Quintero Z.L., Guerau-de-Arellano M., Cassady K.A., Zheng S.G., Yang J., Lane A.N., Song X., Fan T.W, & Wang R. (2020). Inosine is an alternative carbon source for CD8+-T-cell function under glucose restriction. Nature Metabolism, 2(7), 635-647.
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