A pre-treatment consisting of hydrated heating at 121 °C in 10% citrate buffer for 20 min preceded the endogenous peroxidase blocking (DAKO, Glostrup, Denmark) for 5 min and incubation with different primary antibodies: polyclonal IL-1α (1:100, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA), polyclonal IL-1RN (1:100, overnight 4 °C; Sigma, St. Louis, MO, USA), monoclonal 8H12 (1:20, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA), or polyclonal IFNGR1 (1:1200, overnight 4 °C; ThermoFisher Scientific, Waltham, MA, USA) were used.
Polyclonal ifngr1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Polyclonal IFNGR1 is a laboratory reagent used for research purposes. It is a protein that binds to the interferon gamma receptor 1 (IFNGR1) and is used to study the function and expression of this receptor. The product is intended for research use only and its specific applications should be determined by the user.
Automatically generated - may contain errors
2 protocols using polyclonal ifngr1
1
Immunohistochemical Analysis of Inflammatory Markers
2
Immunohistochemical staining of cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A pre-treatment consisting of hydrated heating at 121 °C in citrate buffer 10% for 20 min preceded the endogenous peroxidase blocking (DAKO, Glostrup, Denmark) for 5 min and incubation overnight 4 °C with different primary antibodies: polyclonal IL-1α (1:100; ThermoFisher Scientific, Waltham, MA, USA), polyclonal IL-1RN (1:100, Sigma, St. Louis, MO, USA), monoclonal 8H12 (1:40; ThermoFisher Scientific, Waltham, MA, USA) or polyclonal IFNGR1 (1:200, ThermoFisher Scientific, Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!