P2 hAMSCs and P1 BMSCs were seeded in 12‐well plates at a density of 2 × 104 with LG‐DMEM culture medium and incubated at 37°C for 24 h. hAMSCs were treated as mentioned in Section 4.7 , and BMSCs were isolated from mice treated as described in Section 4.3 . High sugar medium (HG‐DMEM), containing 50 mg/L vitamin C (Solarbio), 100 nmol/L dexamethasones (Sigma), and 10% FBS, was used to prepare osteogenic induction medium with 10 mmol/L β‐glycerophosphate (Solarbio) and cartilage induction medium with 10 ng/L transforming growth factor‐β3 (Peprotech). They were used to incubate hAMSCs and BMSCs at room temperature. Toluidine blue staining was used to observe chondrogenesis after 5 days. Osteogenic differentiation was preliminarily observed using alkaline phosphatase and alizarin red staining after 7 and 21 days, respectively. The hAMSCs were cultured in adipogenic induction medium (Procell Life Science & Technology Co., Ltd) for 26 days, and oil red O staining was used to observe the formation of lipid droplets.
Dexamethasones
Manufactured by Merck Group
Sourced in United States
Dexamethasones are synthetic glucocorticoid medications used in a variety of medical applications. They act as anti-inflammatory and immunosuppressant agents. Dexamethasones are commonly used in the treatment of conditions such as rheumatic disorders, allergic reactions, and certain types of cancer.
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Lab products found in correlation
2 protocols using dexamethasones
1
Comparative Analysis of Stem Cell Differentiation
2
Osteogenic Differentiation of BMSCs
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For osteogenic differentiation, BMSCs were incubated in the α-MEM medium, and then the medium was replaced with osteogenic inducing medium (OIM). OIM were made from a culture medium with 0.05 mM vitamin C (Sigma, USA), 10 mM β-glycerophosphate (Sigma, USA) and 1 × 108 M dexamethasones (Sigma, USA). Medium were exchanged every 2 days.
The BMSCs were cultured in 12-well plates with cement-extracts at 1 × 105 cells per well in α-MEM and 10% FBS medium in the cell incubator. After the cell density reached 70–80% approximately, the α-MEM medium was switched to OIM and BMSCs were induced to differentiate for 7/14 days. After that, differentiation of BMSCs was tested with alkaline phosphatase (ALP) staining, ALP activity and alizarin red S (ARS) staining.
ALP activity was checked using an ALP assay kit (Beyotime, China) after 7/14 days of culture. The ALP activity results were standardized with total cell protein and the media containing CPC cement extracts were used as a control. After 7/14 days of culture, cell monolayers were washed twice with PBS and were processed with 10% neutrophilic formalin for 15 min. After washing twice with PBS, the samples were stained according to the protocol of the ALP and ARS assay kit and observed with a light microscope (Olympus, IX71, Japan).
The BMSCs were cultured in 12-well plates with cement-extracts at 1 × 105 cells per well in α-MEM and 10% FBS medium in the cell incubator. After the cell density reached 70–80% approximately, the α-MEM medium was switched to OIM and BMSCs were induced to differentiate for 7/14 days. After that, differentiation of BMSCs was tested with alkaline phosphatase (ALP) staining, ALP activity and alizarin red S (ARS) staining.
ALP activity was checked using an ALP assay kit (Beyotime, China) after 7/14 days of culture. The ALP activity results were standardized with total cell protein and the media containing CPC cement extracts were used as a control. After 7/14 days of culture, cell monolayers were washed twice with PBS and were processed with 10% neutrophilic formalin for 15 min. After washing twice with PBS, the samples were stained according to the protocol of the ALP and ARS assay kit and observed with a light microscope (Olympus, IX71, Japan).
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