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Anti rabbit phosphor irs1

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-rabbit phosphor IRS1 is a laboratory reagent used to detect and quantify phosphorylated insulin receptor substrate 1 (IRS1) in biological samples. It is a specific antibody that binds to the phosphorylated form of IRS1, allowing researchers to study insulin signaling and related cellular processes.

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2 protocols using anti rabbit phosphor irs1

1

Comprehensive Immunoblotting Protocol for Metabolic Signaling

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Primary and secondary antibodies against anti-rabbit AMPK, anti-rabbit phosphor-AMPK, anti-rabbit ACC, anti-rabbit phosphor ACC, anti-rabbit phosphor-hormone sensitive lipase (HSL), anti-rabbit FAS, anti-mouse glucose transporter 4 (GLUT4), anti-rabbit LKB1, anti-rabbit phosphor-LKB1, anti-rabbit CaMKK, anti-rabbit phosphor-CaMKK, anti-rabbit protein kinase A (PKA), anti-rabbit phosphor PKA, anti-rabbit Sirtuin 1 (Sirt1), anti-rabbit PPARγ, anti-rabbit C/EBPα, anti-rabbit C/EBPβ, anti-rabbit insulin receptor substrate 1 (IRS1), anti-rabbit phosphor IRS1 (Ser1101), anti-rabbit phosphor Erk, anti-rabbit uncoupling protein 1 (UCP1), anti-rabbit mammalian target of rapamycin (mTOR), anti-rabbit phosphor-mTOR, anti-rabbit phosphor ribosomal protein S6 kinase (S6K), anti-rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-rabbit IgG, and anti-mouse IgG were purchased from Cell Signaling Technology (Commonwealth of Massachusetts, USA). Anti-rabbit phosphor C/EBPβ was purchased from Abcam (Cambridge, UK); HSL was purchased from Sigma (State of Missouri, U.S.A.); and anti-rabbit IRβ, anti-rabbit phosphor IRβ, and anti-rabbit phosphor IRS1 (Tyr465) were purchased from Santa Cruz Biotechnology (Texas, USA).
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2

Comprehensive Immunoblotting Analysis of Insulin Signaling Pathway

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Western blotting was performed with the following commercially available antibodies: antirabbit Akt, anti-rabbit phosphor-Akt (Ser473) anti-rabbit phosphor-Akt (Thr308), anti-rabbit FoxO1, anti-rabbit phosphor-FoxO1, anti-rabbit phosphor-IRS1 (Ser1101), anti-rabbit phosphormammalian target of rapamycin (mTOR) (Ser2448), anti-rabbit phosphor-S6 kinase (S6K) (Ser240/244), anti-rabbit phosphor-janus activating kinase 2 (JAK2) (Ser1007/1008), anti-rabbit AMPK, anti-rabbit phosphor-AMPK, anti-rabbit phosphor-CaMKK, anti-rabbit phosphor-LKB1, antirabbit phosphor-protein kinase A (PKA), anti-rabbit ACC, anti-rabbit phosphor-ACC, anti-rabbit HSL, anti-rabbit phosphor-HSL, anti-rabbit CCAAT/enhancer-binding protein (C/EBP), anti-rabbit C/EBP, anti-rabbit peroxisome proliferator-activated receptor  (PPAR), anti-rabbit IgG and antimouse IgG from Cell Signaling Technology (Beverly, MA, USA); anti-rabbit POMC, anti-goat agoutirelated protein (Agrp), anti-rabbit phospho-IRS1 (Tyr465), anti-rabbit IRS1 and ant-goat IgG from Santa Cruz Biotechnology; and anti-mouse -Actin from Sigma (St. Louis, MO, USA).
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