To probe cellulose microfibrils in cell walls, the primary roots of wild-type and mutant seedlings were subjected to AFM as described previously (Zhang et al. 2019 (link)). The root tips were cut and treated in a peracetic acid solution (11%, v/v) at 85 °C for 3 h. After extensive rinsing, the exposed cell walls of detached root cortex cells were imaged in the air by using a multimode scanning probe microscope (MM-SPM; Bruker) with an advanced NanoScope V Controller (Veeco). All obtained images were scanned in 1-μm scale at 512 × 512 pixels using a ScanAsyst-Air probe (Bruker). The raw images were flattened to remove tilt or bow and then exported in the TIFF format using Nanoscope Analysis (version 1.8; Bruker).
Nanoscope analysis version 1.8
Manufactured by Bruker
Sourced in United States
Nanoscope Analysis (version 1.8) is a software application developed by Bruker for the analysis and processing of data from atomic force microscopy (AFM) instruments. The core function of the software is to provide tools for visualizing, analyzing, and interpreting AFM data.
Automatically generated - may contain errors
2 protocols using nanoscope analysis version 1.8
1
Probing Plant Cell Wall Microfibrils
2
Nanomechanical Characterization of HUVEC Monolayer
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A dimension icon atomic force microscope (AFM) (Bruker, BioScope Catalyst, Billerica, MA, USA) operated in PeakForce Quantitative Nanomechanics (QNM) mode, was employed to collect the topographical and modulus images of the HUVEC monolayer as previously described. 23, 24 Briefly, the QNM-Live Cell (PFQNM-LC) probes (Bruker AFM Probes, Camarillo, CA, USA) were used with a spring constant of 0.07 N/m, tip length of 17 μm, and tip radius of 65 nm. The scanning size was 100 × 100 μm 2 with a resolution of 256 × 256 pixels, and the entire surface of a HUVEC cell was scanned point-by-point to obtain the individual elastic modulus values, at an indentation depth of 400-500 nm. All experiments were performed with ≥3 independent repeats at 25°C. A HUVEC cell was divided into two regions of the cell body, and cell periphery, in the height image. Average Young's modulus is calculated as the mean of the values at each point in the region. Data were analyzed using Nanoscope Analysis Version 1.8 (Bruker, Santa Barbara, CA, USA).
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