The largest database of trusted experimental protocols

Nanoscope analysis version 1.8

Manufactured by Bruker
Sourced in United States

Nanoscope Analysis (version 1.8) is a software application developed by Bruker for the analysis and processing of data from atomic force microscopy (AFM) instruments. The core function of the software is to provide tools for visualizing, analyzing, and interpreting AFM data.

Automatically generated - may contain errors

2 protocols using nanoscope analysis version 1.8

1

Probing Plant Cell Wall Microfibrils

Check if the same lab product or an alternative is used in the 5 most similar protocols
To probe cellulose microfibrils in cell walls, the primary roots of wild-type and mutant seedlings were subjected to AFM as described previously (Zhang et al. 2019 (link)). The root tips were cut and treated in a peracetic acid solution (11%, v/v) at 85 °C for 3 h. After extensive rinsing, the exposed cell walls of detached root cortex cells were imaged in the air by using a multimode scanning probe microscope (MM-SPM; Bruker) with an advanced NanoScope V Controller (Veeco). All obtained images were scanned in 1-μm scale at 512 × 512 pixels using a ScanAsyst-Air probe (Bruker). The raw images were flattened to remove tilt or bow and then exported in the TIFF format using Nanoscope Analysis (version 1.8; Bruker).
+ Open protocol
+ Expand
2

Nanomechanical Characterization of HUVEC Monolayer

Check if the same lab product or an alternative is used in the 5 most similar protocols
A dimension icon atomic force microscope (AFM) (Bruker, BioScope Catalyst, Billerica, MA, USA) operated in PeakForce Quantitative Nanomechanics (QNM) mode, was employed to collect the topographical and modulus images of the HUVEC monolayer as previously described. 23, 24 Briefly, the QNM-Live Cell (PFQNM-LC) probes (Bruker AFM Probes, Camarillo, CA, USA) were used with a spring constant of 0.07 N/m, tip length of 17 μm, and tip radius of 65 nm. The scanning size was 100 × 100 μm 2 with a resolution of 256 × 256 pixels, and the entire surface of a HUVEC cell was scanned point-by-point to obtain the individual elastic modulus values, at an indentation depth of 400-500 nm. All experiments were performed with ≥3 independent repeats at 25°C. A HUVEC cell was divided into two regions of the cell body, and cell periphery, in the height image. Average Young's modulus is calculated as the mean of the values at each point in the region. Data were analyzed using Nanoscope Analysis Version 1.8 (Bruker, Santa Barbara, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!