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Goat antipecam 1 antibody sc 1506

Manufactured by Santa Cruz Biotechnology

Goat antiPECAM-1 antibody (sc-1506) is a primary antibody produced in goat that recognizes the PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) protein. PECAM-1 is a cell adhesion molecule expressed on the surface of platelets, monocytes, neutrophils, and some types of T cells. This antibody can be used for the detection of PECAM-1 in various applications.

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2 protocols using goat antipecam 1 antibody sc 1506

1

Immunohistochemical Analysis of Protocadherins

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Wild-type C57BL/6J and homozygous Pcdha9 mutant embryos were obtained at E14.5. The embryos were fixed in 4% paraformaldehyde and processed for cryoembedding. Frozen sections were collected for immunostaining. Cryosections of mouse embryonic heart tissue were immunostained with rabbit anti-alpha-protocadherin antibody (190003, Synaptic Systems, 1:500), goat antiPECAM-1 antibody (sc-1506, Santa Cruz Biotechnology, 1:500), and mouse anti-alpha smooth muscle actin antibody (1A4) (ab7817, Abcam, 1:500). Human adult aortic tissues were stained with the alpha-protocadherin antibody and also imaged for elastin autofluorescence. Secondary antibodies included goat anti-rabbit IgG (Invitrogen, #A21429, 1:1,000) and goat anti-mouse IgM (Invitrogen, #A21042, 1:1,000). DAPI was used to label nuclei (D1306, Invitrogen). Sections were imaged with confocal microscopy and quantitatively analyzed with ImageJ.40 (link)
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2

Immunostaining of Pcdha9 Mutant Embryos

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Wild-type C57BL/6J and homozygous Pcdha9 mutant embryos
were obtained at E14.5. The embryos were fixed in 4% paraformaldehyde and
processed for cryoembedding. Frozen sections were collected for immunostaining.
Cryosections of mouse embryonic heart tissue were immunostained with rabbit
anti-alpha-protocadherin antibody (190003, Synaptic Systems, 1:500), goat
antiPECAM-1 antibody (sc-1506, Santa Cruz Biotechnology, 1:500), and mouse
anti-alpha smooth muscle actin antibody (1A4) (ab7817, Abcam, 1:500). Human
adult aortic tissues were stained with the alpha-protocadherin antibody and also
imaged for elastin autofluorescence. Secondary antibodies included goat
anti-rabbit IgG (Invitrogen, #A21429, 1:1,000) and goat anti-mouse IgM
(Invitrogen, #A21042, 1:1,000). DAPI was used to label nuclei (D1306,
Invitrogen). Sections were imaged with confocal microscopy and quantitatively
analyzed with ImageJ.40 (link)
+ Open protocol
+ Expand

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