Caspase 3 antibody

Manufactured by Beyotime

Sourced in China

The Caspase-3 Antibody is a laboratory reagent used for the detection and analysis of the Caspase-3 protein, a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and quantify the Caspase-3 protein in biological samples.

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Lab products found in correlation

Bcl 2 antibody, by Beyotime (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Resveratrol, by Merck Group (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Atp assay kit, by Beyotime (1 mentions) Jc 1 probe, by Beyotime (1 mentions) Mptp assay kit, by Genmed Scientifics (1 mentions)

2 protocols using caspase 3 antibody

Apoptosis and Cell Cycle Analysis

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In this study, we used the following materials: Annexin V-FITC/PI Apoptosis Detection Kit (product lot No. FXP018-100) and Cell Cycle Analysis Kit (product No. FXP021-100) were purchased from Beijing 4A Biotech Co., Ltd. (Beijing, China). Caspase-3 Antibody (product No. AB030), active Caspase-3 Antibody (product No. AC033), active caspase-8 antibody (product No. AC056), active caspase-9 antibody (product No. AC062), Bcl-2 antibody (product No. AB112), and Bax antibody (product No. AB026) were purchased from Beyotime Institute of Biotechnology (Jiangsu, China).

Fu B., Xi S., Wang Y., Zhai X., Wang Y., Gong Y., Xu Y., Yang J., Qiu Y., Wang J., Lu D, & Huang S. (2019). The Protective Effects of Ciji-Hua’ai-Baosheng II Formula on Chemotherapy-Treated H22 Hepatocellular Carcinoma Mouse Model by Promoting Tumor Apoptosis. Frontiers in Pharmacology, 9, 1539.

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Resveratrol Cytotoxic Effects in Vitro

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Resveratrol (purity 99%; Sigma), RPMI-1640 medium, and fetal bovine serum (FBS) were obtained from Invitrogen (Shanghai, China).
MTT Cell Proliferation and Cytotoxicity Assay Kit (catalog No. C0009), Caspase-3 antibody (catalog No. AC033), Bcl-2 antibody (catalog No. AB112), secondary antibody [HRP-labeled goat anti-rabbit IgG (H+L)] (catalog No. A0208), JC-1 probe (catalog No. C2006), ATP assay kit (catalog No. S0026), and BCA protein assay kit (catalog No. P0012) were purchased from Beyotime Institute of Biotechnology (Haimen, China) and MPTP assay kit (catalog No. GMS10095.1) from Genmed Scientifics Inc. (USA) Fluorescein diacetate (FDA) was obtained from Keeasy Economic & Trade Co., Ltd. All other reagents were of analytical grade and were obtained from commercial sources.

Li J.P., Ma Q, & Chen C.M. (2016). Mitochondrial dysfunction in resveratrol-induced apoptosis in QGY-7701 cells. Genetics and molecular research : GMR, 15(1).

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