Zeatin

Tomato transformations followed the protocol by Sun et al. (2006) (link). In brief, after 3 days of co-cultivation, tomato cotyledon segments were placed on a callus-induction medium [MS medium containing 0.3% Gelrite (Wako, Tokyo, Japan), 1.5 mg/l zeatin, 100 mg/l kanamycin, and 375 mg/l Augmentin (GlaxoSmithKline, London, UK)] for 4 weeks. Calli that formed segments were cultured on shoot-induction medium [MS medium containing 0.3% Gelrite (Wako, Tokyo, Japan), 1.0 mg/l zeatin, 100 mg/l kanamycin, and 375 mg/l Augmentin (GlaxoSmithKline, London, UK)] for 4 weeks. The shoots were then placed on rooting medium, which consisted of half-strength MS medium, 0.3% Gelrite (Wako, Tokyo, Japan), 100 mg/L kanamycin, and 375 mg/l Augmentin, for 2 weeks. Tissues were each subcultured for 10–14 days.