We obtained human colorectal cancer cell lines either from our lab, the Chinese Academy of Sciences, or the Shanghai Institute of Biological Sciences. Dulbecco’s Modified Eagle’s Media (Gibco, Thermo Fisher Scientific, Inc.) was used to treat RKO, SW480, and SW620 cells, whereas McCoy’s 5A medium was used to treat HCT116 cells (Nanjing KeyGen Biotech Co., Ltd.). We added 10% foetal bovine serum (Gibco, Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin to the media as supplements. To continue exponential development, subculture was performed every two to three days at 37°C in a humidified environment with 5% CO2 and 95% air.
Hct116 cells
Manufactured by Keygen Biotech
Sourced in China
HCT116 cells are a well-established human colorectal carcinoma cell line. They are commonly used in cancer research and drug discovery studies.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s media, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Keygen Biotech (1 mentions)
Lovo cells, by Keygen Biotech (1 mentions)
Ht 29 cells, by Keygen Biotech (1 mentions)
Hct 8 cells, by Keygen Biotech (1 mentions)
2 protocols using hct116 cells
1
Culturing Human Colorectal Cancer Cells
2
Cell Culture Protocols for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HCT116 cells, RKO cells, SW480 cells, HCT8 cells, HT29 cells, LOVO cells, and FHC cells were obtained from the KeyGEN BioTECH (Nanjing, Jiangsu, China). Cell culture was performed in an atmosphere of 5% CO2 at 37°C.
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