Eia 1781

Manufactured by DRG International

Sourced in Germany

The EIA-1781 is a laboratory equipment designed for performing enzyme-linked immunosorbent assays (ELISA). It is a compact and versatile instrument that can be used in a variety of research and diagnostic applications. The EIA-1781 is capable of automating the ELISA process, including sample pipetting, plate washing, and absorbance measurement. The instrument is equipped with a microplate reader that can accurately detect and quantify target analytes in biological samples.

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Lab products found in correlation

Ab100695, by Abcam (1 mentions) Ezrmcp2 21k, by Merck Group (1 mentions)

5 protocols using eia 1781

Thyroid Hormone Measurement Protocol

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TSH concentration was measured as previously described (Pohlenz et al. 1999) (link). Serum total T 4 (TT 4 ) and free T 3 (FT 3 ) concentrations were measured using commercial ELISA kits according to the manufacturer's instructions (DRG Instruments GmbH, Marburg, Germany; FT 3 : EIA-2385; TT 4 : EIA-1781) with a detection limit of 0.5 µg/dL for TT 4 and 0.05 pg/mL for FT 3 .

Latteyer S., Christoph S., Theurer S., Hönes G.S., Schmid K.W., Führer D, & Moeller L.C. (2019). Thyroxine promotes lung cancer growth in an orthotopic mouse model. Endocrine-related cancer, 26(6).

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Serum Biomarker Analysis in Preclinical Study

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TT4 serum concentration (n = 6–8) was measured using an ELISA kit according to the manufacturer’s instructions (EIA 1781, DRG Instruments GmbH, Marburg, Germany) with an intra-assay coefficient of variation of 3.1–4.3% and an inter-assay coefficient of variation of 2.4–4.5%. Serum enzyme activities (n = 4) of alanine transaminase (ALT) and aspartate transaminase (AST) and total cholesterol concentrations (n = 4–8) were analyzed by LABOKLIN GmbH & Co. KG (LABOKLIN, Bad Kissingen, Germany).

Kube I., Kowalczyk M., Hofmann U., Ghallab A., Hengstler J.G., Führer D, & Zwanziger D. (2022). Hepatobiliary Thyroid Hormone Deficiency Impacts Bile Acid Hydrophilicity and Aquaporins in Cholestatic C57BL/6J Mice. International Journal of Molecular Sciences, 23(20), 12355.

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Measuring Serum Thyroid Hormones

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Serum concentrations of total T 3 (tT3) and total T 4 (tT4) were determined with commercially available ELISA Kits (total Thyroxine ELISA, EIA-1781, DRG Instruments GmbH, Marburg, Germany, and total T3 ELISA, DNOV053, NovaTec Immundiagnostica GmbH, Dietzenbach, Germany) and experiments were performed in accordance with manufacturer's instructions.

Gachkar S., Nock S., Geissler C., Oelkrug R., Johann K., Resch J., Rahman A., Arner A., Kirchner H, & Mittag J. (2019). Aortic effects of thyroid hormone in male mice. Journal of molecular endocrinology, 62(3).

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Serum T4 and T3 Quantification

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Serum concentrations were measured using commercially available ELISA kits for total T4 (EIA-1781; DRG Instruments GmbH, Germany) and total T3 (DNOV053; NovaTec Immundiagnostica GmbH, Germany).

Dore R., Watson L., Hollidge S., Krause C., Sentis S.C., Oelkrug R., Geißler C., Johann K., Pedaran M., Lyons G., Lopez-Alcantara N., Resch J., Sayk F., Iwen K.A., Franke A., Boysen T.J., Dalley J.W., Lorenz K., Moran C., Rennie K.L., Arner A., Kirchner H., Chatterjee K, & Mittag J. (2023). Resistance to thyroid hormone induced tachycardia in RTHα syndrome. Nature Communications, 14, 3312.

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Serum Biomarker Quantification Protocol

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Serum IGF-1 (ab100695, Abcam, Germany), C-peptide (C-peptide 2, EZRMCP2-21K, Millipore, Merck Darmstadt, Germany), and total T4 (EIA 1781, DRG Instruments GmbH, Marburg, Germany) were determined by commercial ELISA kits.

Oelkrug R., Herrmann B., Geissler C., Harder L., Koch C., Lehnert H., Oster H., Kirchner H, & Mittag J. (2017). Dwarfism and insulin resistance in male offspring caused by α1-adrenergic antagonism during pregnancy. Molecular Metabolism, 6(10), 1126-1136.

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