Ova b16

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

The OVA-B16 is a laboratory instrument designed for the culturing and maintenance of cell lines. It provides a controlled environment for the growth and proliferation of various cell types, including those derived from ovarian and melanoma tissues. The core function of the OVA-B16 is to maintain optimal conditions for cell culture, such as temperature, humidity, and gas composition, to support the growth and viability of the cells under study.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Mcf 7, by National Collection of Authenticated Cell Cultures (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Polyethylene glycol 400 peg400, by Sinopharm (1 mentions) L kynurenine sulfate salt, by Merck Group (1 mentions) 1 methyl l tryptophan, by Merck Group (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Hek293t, by National Collection of Authenticated Cell Cultures (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Sgc 7901, by National Collection of Authenticated Cell Cultures (1 mentions) Thp 1, by National Collection of Authenticated Cell Cultures (1 mentions) Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)

5 protocols using ova b16

In Vitro Cultivation of Tumor Cell Lines

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Mouse tumor cell lines B16 (melanoma), OVA-B16 (melanoma), C26 (colon cancer) and 4 T1 (breast cancer) were purchased from the China Center for Type Culture Collection (Beijing, China) and cultured in RPMI 1640 (Thermo Fisher, MA, USA) with 10% fetal bovine serum (FBS) (Gibco, MA, USA), with the exception of 4 T1 cells, which were grown in DMEM medium (Gibco, MA, USA) with 10% FBS.
Carboxyamidotriazole was synthesized by the Institute of Materia Medica, Chinese Academy of Medical Sciences (Beijing, China). Polyethylene glycol 400 (PEG400) was obtained from Sinopharm Chemical Reagent Beijing (Beijing, China). 1-Methyl-L-tryptophan, 3′, 4′-dimethoxyflavone and L-kynurenine sulfate salt were purchased from Sigma-Aldrich (Saint Louis, USA).

Shi J., Chen C., Ju R., Wang Q., Li J., Guo L., Ye C, & Zhang D. (2019). Carboxyamidotriazole combined with IDO1-Kyn-AhR pathway inhibitors profoundly enhances cancer immunotherapy. Journal for Immunotherapy of Cancer, 7, 246.

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Murine and Human Tumor Cell Lines

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Murine tumor cell lines B16, OVA-B16, MC38, 4T1 and H22 and human tumor cell lines A375, Hela and HEK-293T were purchased from the China Center for Type Culture Collection and cultured in RPMI 1640 or DMEM (Gibco) supplemented with 10% FBS (Gibco). All cells were cultured at 37 °C in 5% CO₂ and routinely tested for mycoplasma.

Ma J., Tang L., Tan Y., Xiao J., Wei K., Zhang X., Ma Y., Tong S., Chen J., Zhou N., Yang L., Lei Z., Li Y., Lv J., Liu J., Zhang H., Tang K., Zhang Y, & Huang B. (2024). Lithium carbonate revitalizes tumor-reactive CD8+ T cells by shunting lactic acid into mitochondria. Nature Immunology, 25(3), 552-561.

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Murine Cell Line Maintenance

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Female SCIDbeige or NSG mice, 6 to 8 weeks old, were purchased from the Center of Medical Experimental Animals of the Chinese Academy of Medical Science (Beijing, China). OTI transgenic mice were gifted by H. Zhang (Sun Yatsen University). Pmel1 transgenic mice were presented by Y. Wan (Third Military Medical University). These animals were maintained in the Animal Facilities of Chinese Academy of Medical Science under pathogenfree conditions. THP1 (acute monocytic leukemia cell line), SGC7901 (gastric adenocarci noma cell line), and MCF7 (breast cancer cell line) and mouse tumor cell lines B16 and OVAB16 (melanoma) were purchased from the China Center for Type Culture Collection (Beijing, China). Human tumor cell lines Raji (Burkitt's lymphoma cell line) and Nalm6 (acute lymphocytic leukemia cell line) were gifted by M. Wang (Chinese Academy of Medical Sciences and Peking Union Medical College). These cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific) with 10% fetal bovine serum (FBS) (Gibco, USA), 2 mM lglutamine (Gibco, USA), and 1% penicillinstreptomycin in a hu midified atmosphere containing 5% CO 2 at 37°C.

Liu Y., Fang Y., Chen X., Wang Z., Liang X., Zhang T., Liu M., Zhou N., Lv J., Tang K., Xie J., Gao Y., Cheng F., Zhou Y., Zhang Z., Hu Y., Zhang X., Gao Q., Zhang Y, & Huang B. (2020). Gasdermin E-mediated target cell pyroptosis by CAR T cells triggers cytokine release syndrome. Science immunology, 5(43).

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Cell Culture of Melanoma, Kidney, and Breast Cancer

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OVA-B16 (mouse melanoma), 293T (human embryonic kidney cell line), and MCF-7 (human breast cancer cell line) cells were purchased from the China Center for Type Culture Collection (Beijing, China) and cultured in RPMI 1640 medium (Thermo Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Scientific, USA).

Liu Y., Zhang T., Zhou Y., Li J., Liang X., Zhou N., Lv J., Xie J., Cheng F., Fang Y., Gao Y., Wang N, & Huang B. (2018). Visualization of perforin/gasdermin/complement-formed pores in real cell membranes using atomic force microscopy. Cellular and Molecular Immunology, 16(6), 611-620.

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Authenticated Cell Lines for Tumor Models

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Mouse tumor cell lines B16, OVA-B16 (melanoma), MC38 (colon cancer), and 4T1 (breast cancer) and human tumor cell lines A375 (melanoma), HepG2 (hepatocellular cancer), and MCF7 (breast cancer) were purchased from China Center for Type Culture Collection (Beijing, China) and cultured in RPMI-1640 (Thermo Scientific) with 10% fetal bovine serum (FBS; Gibco). Cells were tested for Mycoplasma detection, interspecies cross contamination and authenticated by isoenzyme and short-tandem repeat analyses in Cell Resource Centre of Peking Union Medical College before the study. Cell lines used in the experiments were within 20 passages.

Liu Y., Zhang T., Zhang H., Li J., Zhou N., Fiskesund R., Chen J., Lv J., Ma J., Zhang H., Tang K., Cheng F., Zhou Y., Zhang X., Wang N, & Huang B. (2021). Cell Softness Prevents Cytolytic T-cell Killing of Tumor-Repopulating Cells. Cancer research, 81(2).

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