Anti glut4 g4048

Manufactured by Merck Group

Anti-GLUT4 (G4048) is a laboratory reagent used for the detection and quantification of the GLUT4 glucose transporter protein in various biological samples. It is a specific antibody that binds to the GLUT4 protein, enabling its identification and measurement through various analytical techniques.

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2 protocols using anti glut4 g4048

Adipogenesis Regulation by Insulin and Signaling

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Insulin, 3-isobutyl-1-methylxanthine (IBMX), dexamethasone (Dex), and Oil Red O were obtained from Sigma (St. Louis, MO, USA). 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose] (2-NBDG) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Anti-Akt (#9272; 1:500), anti-phospho-Akt (p-Akt; Thr308; #9275; 1:500), and anti-C/EBPα (#2295; 1:1000) polyclonal antibodies were from Cell Signaling (Danvers, MA, USA). Anti-GLUT4 (G4048; 1:1000) polyclonal and β-actin (AC-15; A1978; 1:3000) monoclonal antibodies were from Sigma. Anti-PPARγ (H-100; 1:1000), anti-fatty acid synthase (FAS; H-300; 1:1000), anti-IRS-1 (C-20; 1:500), and anti-phospho-IRS-1 (Tyr-632; 1:500) polyclonal antibodies and anti-mouse, anti-rabbit, and anti-goat IgG, horseradish peroxidase-linked secondary antibodies (1:1000) were from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Matsumoto C., Koike A., Tanaka R, & Fujimori K. (2019). A Limonoid, 7-Deacetoxy-7-Oxogedunin (CG-1) from Andiroba (Carapa guianensis, Meliaceae) Lowers the Accumulation of Intracellular Lipids in Adipocytes via Suppression of IRS-1/Akt-Mediated Glucose Uptake and a Decrease in GLUT4 Expression. Molecules, 24(9), 1668.

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Comprehensive Glutathione Pathway Analysis

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All chemicals, unless specified, were purchased from Sigma. Antibodies against glutathione [D8] (ab19534), GCLC (ab53179), GCLM [EPR6667] (ab126704), glutathione synthetase [EPR6562] (ab124811), glutathione reductase (ab16801), xCT or Slc7a11 (ab37185), PPARα (ab24509), PPARγ (ab19481), PGC1α (ab54481), Vitamin D receptor (ab3508), and β-actin HRP (ab49900) were purchased from Abcam. The anti-CTH (WH0001491M) and anti-GLUT4 (G4048) were purchased from Sigma Aldrich. Goat anti- mouse HRP (170–6516) was purchased from Biorad and the goat anti-rabbit HRP (12–348) from Millipore. Pierce^™ protein A/G agarose was purchased from Thermo Scientific.

Parsanathan R, & Jain S.K. (2018). Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilization in C2C12 mouse myotubes. Free radical research, 52(2), 288-303.

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