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Hrp conjugated rabbit and mouse secondary antibodies

Manufactured by GE Healthcare
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HRP conjugated rabbit and mouse secondary antibodies are laboratory reagents used in immunoassays and other immunochemical techniques. They bind to the constant regions of primary antibodies raised in rabbit or mouse, and are conjugated with the enzyme Horseradish Peroxidase (HRP). This allows for the detection and amplification of the target antigen signal in the assay.

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Lab products found in correlation

Ha hrp, by Roche (1 mentions) Igg1 pe, by R&D Systems (1 mentions) P gsk3β, by Cell Signaling Technology (1 mentions) Actin antibody, by Santa Cruz Biotechnology (1 mentions) Igg1 fitc, by BD (1 mentions) Taci pe, by R&D Systems (1 mentions) Anti traf6, by Abcam (1 mentions) P mtor s2481, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Salubrinal, by Santa Cruz Biotechnology (1 mentions) Recombinant human tnf, by R&D Systems (1 mentions) P mk2, by Cell Signaling Technology (1 mentions) Vegf165, by R&D Systems (1 mentions) Phosphatase inhibitor cocktails 1 and 2, by Merck Group (1 mentions) P iκb, by Cell Signaling Technology (1 mentions) Eif2α, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Recombinant human bmp 2, by Merck Group (1 mentions) Mouse igg, by R&D Systems (1 mentions) Sb202190, by Merck Group (1 mentions)

2 protocols using hrp conjugated rabbit and mouse secondary antibodies

1

Western Blot Antibody Reagents

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HRP conjugated rabbit and mouse secondary antibodies were purchased from GE Healthcare (Waukesha, WI), while goat-HRP and actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against Akt, pAktS473, pAktT308, GSK3β, pGSK3β, MTOR and pMTORS2481, and pMTORS2488 were purchased from Cell Signaling (Beverly, MA). HA-HRP was purchased from Roche (Indianapolis, IN), while anti-TRAF6 and BAFF-R FITC antibodies were purchased from Abcam (Cambridge, UK). IgG1 FITC was purchased from Becton Dickinson (Franklin Lakes, NJ), while IgG1 PE and TACI PE were purchased from R&D Systems (Minneapolis, MN).
Secreto F., Manske M., Price-Troska T., Ziesmer S., Hodge L.S., Ansell S.M., Cerhan J.R, & Novak A.J. (2014). BAFF-R Specific Activation of TRAF6 and the PI3K-Pathway in Lymphoma B Cells. Leukemia & lymphoma, 55(8), 1884-1892.
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2

Optimizing Angiogenic Signaling Pathways

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Recombinant human BMP-2, anti–α-tubulin antibody, anti–β-actin antibody, collagenase IA-S, protease inhibitor cocktail, and phosphatase inhibitor cocktails I and II were purchased from Sigma-Aldrich. Recombinant human TNF, GM-CSF, VEGF165, PDGF-BB, and mouse IgG were purchased from R&D Systems. BMPR2 and Mac-3 antibodies were purchased from BD, and S-6, L13a, p38, p-p38, MK2, p-MK2, I-κB, p–I-κB, eIF2α, and p-eIF2α antibodies were purchased from Cell Signaling Technology. GADD34, protein phosphatase-1, GM-CSFRα, HuR, and TIA-1 antibodies (4H1) and salubrinal were obtained from Santa Cruz Biotechnology, Inc., and G3BP antibody was obtained from BD. SB202190 was purchased from EMD Millipore. GM-CSF and TNF antibodies for immunohistochemistry were obtained from Abcam, and CD31, CD34, CD68, and α-SM actin antibodies and normal rabbit Ig were obtained from Dako. HRP-conjugated rabbit and mouse secondary antibodies and ECL and ECL Plus kits were ordered from GE Healthcare. Allophycocyanin (APC)-human CD31 and PE–GM-CSFRα antibodies were purchased from eBioscience. Recombinant murine GM-CSF was obtained from PeproTech.
Sawada H., Saito T., Nickel N.P., Alastalo T.P., Glotzbach J.P., Chan R., Haghighat L., Fuchs G., Januszyk M., Cao A., Lai Y.J., Perez V.D., Kim Y.M., Wang L., Chen P.I., Spiekerkoetter E., Mitani Y., Gurtner G.C., Sarnow P, & Rabinovitch M. (2014). Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension. The Journal of Experimental Medicine, 211(2), 263-280.
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