MBP was prepared from bovine brains according to [28 ]. The obtained protein was purified by reverse phase HPLC on a C4 10/250 column (Macherey-Nagel). Actin from porcine muscle, lysozyme from chicken egg, calmodulin from bovine brain, and BSA were obtained from Sigma. Recombinant histone H1.3 was obtained from E. coli, and recombinant human ubiquitin and recombinant human K48-tetraubiquitin were obtained from Boston Biochem. GA (Copaxone) is a commercially available drug from Teva; for the experiments, it was desalted into 20 mM Tris-HCl pH 7.5 using a HiTrap Desalt column (GE Healthcare Life Sciences).
Actin from porcine muscle
Manufactured by Merck Group
Sourced in United States
Actin from porcine muscle is a laboratory product offered by Merck Group. It is a protein that plays a crucial role in the contractile mechanism of muscle cells. This product is derived from the skeletal muscle of pigs and can be used for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Calmodulin, by Merck Group (1 mentions)
Recombinant human ubiquitin, by R&D Systems (1 mentions)
Lysozyme from, by Merck Group (1 mentions)
Hitrap desalt column, by GE Healthcare (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Formononetin, by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
Daidzein, by Merck Group (1 mentions)
Biochanin a, by Merck Group (1 mentions)
2 protocols using actin from porcine muscle
1
Purification and Characterization of Biomolecules
2
Porcine Muscle Actin Purification
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Actin from porcine muscle ~90% lyophilized powder containing Tris, ATP and CaCl2, daidzein ≥98%, biochanin A ≥95%, genistein ≥98% and formononetin ≥99%, were purchased from Sigma Aldrich (St. Louis, MO, USA).
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