FOXP3‐eFluor 450 (eBioscience, Cat.# 48‐5773‐82, clone: FJK‐16s, 1:333), CD95 (Fas)‐Brilliant Violet 605 (BioLegend, Cat.# 152612, clone: SA367H8, 1:80), CD185 (CXCR5)‐Brilliant Violet 711 (BioLegend, Cat.# 145529, clone: L138D7, 1:40), CD4‐PerCP/Cyanine5.5 (BioLegend, Cat.# 100434, clone: GK1.5, 1:80), CD279 (PD‐1)‐PE (BioLegend, Cat.# 135206, clone: 29F.1A12, 1:20), CD25‐PE/Cy5 (BioLegend, Cat.# 102010, clone: PC61, 1:80), GL7‐eFluor 660 (eBioscience, Cat.# 50‐5902‐82, clone: GL7, 1:80), CD19‐APC/Cyanine7 (BioLegend, Cat.# 115530, clone: 6D5, 1:20), and LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher Scientific, Cat.# L34967, 1:7500).
Cd279 pd 1 pe
Manufactured by BioLegend
Sourced in Denmark
CD279 (PD-1)-PE is a lab equipment product produced by BioLegend. It is a fluorescently labeled antibody that binds to the PD-1 (Programmed Cell Death 1) receptor, which is expressed on the surface of activated T cells, B cells, and myeloid cells. The PE (Phycoerythrin) fluorescent dye is conjugated to the anti-PD-1 antibody, allowing for the detection and analysis of PD-1-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable yellow dead cell stain, by Thermo Fisher Scientific (2 mentions)
Foxp3 efluor450, by Thermo Fisher Scientific (1 mentions)
Cd25 pe cy5, by BioLegend (1 mentions)
Gl7 efluor 660, by Thermo Fisher Scientific (1 mentions)
Cd4 percp cyanine5, by BioLegend (1 mentions)
Brilliant violet 711, by BioLegend (1 mentions)
Cd4 pacific blue, by BioLegend (1 mentions)
Cd3 apc cyanine7, by BioLegend (1 mentions)
Cd45 percp, by BioLegend (1 mentions)
Cd8a fitc, by BioLegend (1 mentions)
Cd274 pd l1 apc, by BioLegend (1 mentions)
Cd4 percp, by BioLegend (1 mentions)
Cd27 apc, by Thermo Fisher Scientific (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
True nuclear transcription factor buffer set, by BioLegend (1 mentions)
Annexin 5 fitc, by BioLegend (1 mentions)
Cd3 apc fire750, by BioLegend (1 mentions)
Cd4 apc, by BioLegend (1 mentions)
Fv10i doc, by Olympus (1 mentions)
Facscanto 2, by BD (1 mentions)
6 protocols using cd279 pd 1 pe
1
Multiparametric Flow Cytometry Analysis of T-Cell Subsets
2
Comprehensive Immune Profiling by Flow Cytometry
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CD4‐Pacific Blue (BioLegend, Cat.# 100531, clone: RM4‐5, 1:50), CD8a‐FITC (BioLegend, Cat.# 100706, clone: 53‐6.7, 1:50), CD45‐PerCP (BioLegend, Cat.# 103130, clone: 30‐F11, 1:80), CD279 (PD‐1)‐PE (BioLegend, Cat.# 135206, clone: 29F.1A12, 1:20), CD3‐APC/Cyanine7 (BioLegend, Cat.# 100222, clone: 17A2, 1:80), and LIVE/DEAD Fixable Yellow Dead Cell Stain (Thermo Fisher Scientific, Cat.# L34967, 1:7500).
3
Analyzing PD-1 and PD-L1 Expression in RA
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PD-1 expression on CD4 T cells and PD-L1 expression on CD1c mDCs of RA patients were analysed by flow cytometry using a FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA). Ex vivo or cultured mDCs were stained with CD1c phycoerythrin (CD1c-PE; BD Biosciences), CD19 peridinin chlorophyll (CD19-PerCP; BioLegend) and CD274 (PD-L1)-APC (BioLegend). mDCs were gated as CD1c-positive and CD19-negative. Ex vivo CD4 T cells were stained with CD45RO fluorescein isothiocyanate (CD45RO-FITC; Dako, Glostrup, Denmark), CD27-APC (Invitrogen), CD279 (PD-1)-PE and CD4-PerCP (BioLegend) using isotype antibodies or autofluorescence as controls. All samples were analysed using FlowJo software (TreeStar, Ashland, OR, USA). To compare mean fluorescence intensity (MFI) values, the autofluorescence intensity was subtracted from the MFI of the stains to reveal true expression values.
4
Quantification of DNA Damage in T Cells
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The following monoclonal antibodies were purchased from BioLegend: anti-γH2AX-Alexa 488, Annexin V-FITC, CD3-APC/Fire750, CD4-APC, CD8-APC/Fire750, and CD279 (PD-1)-PE.
Immuno uorescence detection of γH2AX nuclear foci CD3+ T cells cultured in 24-well plates were collected in centrifuge tubes and washed twice with PBS. T cells were xed and permeabilized using a True-Nuclear™ Transcription Factor Buffer Set (BioLegend, USA). T cells were then incubated for 30 min with PBS containing 1% BSA and an anti-γH 2 AX antibody conjugated to Alexa 488 at RT. T cells were washed thrice with PBS and counterstained with DAPI. The immunostained cells were subjected to ow cytometry analysis and confocal laser scanning microscopy (FLUOVIEW FV10i-DOC, OLYMPUS, Japan). BD FACS Canto II (BD Biosciences, USA) was used to measure the forward scatter, side scatter, and expression levels of γH2AX nuclear foci. Data were analyzed using the Flowing Software (Turku Bioscience, Finland).
Immuno uorescence detection of γH2AX nuclear foci CD3+ T cells cultured in 24-well plates were collected in centrifuge tubes and washed twice with PBS. T cells were xed and permeabilized using a True-Nuclear™ Transcription Factor Buffer Set (BioLegend, USA). T cells were then incubated for 30 min with PBS containing 1% BSA and an anti-γH 2 AX antibody conjugated to Alexa 488 at RT. T cells were washed thrice with PBS and counterstained with DAPI. The immunostained cells were subjected to ow cytometry analysis and confocal laser scanning microscopy (FLUOVIEW FV10i-DOC, OLYMPUS, Japan). BD FACS Canto II (BD Biosciences, USA) was used to measure the forward scatter, side scatter, and expression levels of γH2AX nuclear foci. Data were analyzed using the Flowing Software (Turku Bioscience, Finland).
5
Characterization of Glioblastoma Tumor Immune Landscape
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SB28 GBM-bearing mouse was euthanized following IACUC guidelines, and the brain tumor was dissected. Tumor tissues were dissociated to generate single-cell suspensions using a Brain Tumor Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. Cell numbers were counted and incubated with CD45 MicroBeads (Miltenyi Biotec) for 15 min at 4°C. Live CD45+ cell fractions were enriched by running cell suspensions through MACS Columns in a Magnetic MACS Separator (Miltenyi Biotec). CD45+CD11b+ cell fractions were then enriched using CD11b MicroBeads (Miltenyi Biotec). Two antibody panels were used for flow cytometry analysis: the myeloid TME composition, anti-mouse fluorescein isothiocyanate (FITC)–CD45 (1:200), allophycocyanin (APC)-Ly6C (1:100), PerCP-CD11b (1:100) (BioLegend), and phycoerythrin (PE) donkey anti-rabbit immunoglobulin G (IgG) (1:200; BioLegend) and Trem2 polyclonal antibody (1:100; Bioss); the lymphoid TME component, anti-mouse APC-CD3 (1:50), PerCP-CD8 (1:100), and PE-CD279 (PD-1) (1:20) (BioLegend). Briefly, cells were incubated with TruStain FcX-Fc blocker CD16/32 (BioLegend) for 15 min at room temperature. Then, cells were stained with different panels of antibodies or isotype controls. Samples were read on LSRFortessa (BD) and analyzed using FlowJo_v10.8.1 software.
6
RABV Strain LBNSE Characterization
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Female ICR mice were purchased from the laboratory animal center of Huazhong Agricultural University, Wuhan, China. The recombinant RABV strain LBNSE was derived from the Street Alabama Dufferin (SAD)‐L16, which is widely applied for the development of vaccine.18 LBNSE with two mutations in the G protein, N194K and R333E, was proliferated in BSR cells.18 , 19 The rabies challenge virus strain‐11 (CVS‐11) was proliferated in BSR cells, too. The BSR cells, a cloned cell line come from BHK‐21 cells, were cultured at 37°C in Dulbecco's modified Eagle's medium (DMEM) (Mediatech, USA) containing 10% fetal bovine serum (FBS, Gibco) and antibiotics (penicillin‐streptomycin solution, 100×) (Beyotime, Wuhan, China). Fluorescein isothiocyanate (FITC)‐conjugated the antibodies against the RABV‐nucleoprotein (N) were obtained from FujiRab (Melvin, PA). Samples of dLNs were stained with monoclonal antibodies for flow cytometry, including FITC‐CD4 (BioLegend), APC‐CD185 (CXCR5) (BioLegend), PE‐CD279 (PD‐1) (BioLegend), FITC‐CD45R/B220 (BioLegend), Alexa Fluor 647‐GL7 (BioLegend), and PE‐CD95 (APO‐1/Fas) (eBioscience).
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