Blood glucose levels were measured with Antisense III (Horiba Ltd., Kyoto, Japan). Plasma insulin levels were determined using a mouse insulin enzyme-linked immunosorbent assay kit (Morinaga-Seikagaku Co. Ltd., Yokohama, Japan). Insulin in the medium and its content was determined using the HTRF insulin assay kit (Cisbio Bioassays, Bagnols-sur-Cèze, France).
Antisense 3
Manufactured by Horiba
Sourced in Japan
The Antisense III is a lab equipment product by Horiba. It is designed for the detection and analysis of nucleic acid sequences. The core function of the Antisense III is to facilitate the hybridization of complementary nucleic acid strands, enabling the identification and characterization of specific genetic targets.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using antisense 3
1
Glucose and Insulin Measurement Protocols
2
Glucose and Insulin Sensitivity Assays
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Glucose-tolerance tests were performed after a 16-h fast. Blood glucose concentrations were measured by a blood glucose test meter (Antisense III; HORIBA Medical, Kyoto, Japan) at 0, 15, 30, 60, 90, and 120 min after intraperitoneal injection of glucose (1.5 mg/g body weight). insulin sensitivity was assessed using an insulin-tolerance test. After 6 h of fasting, insulin (1 U/kg body weight; Eli Lilly, Indianapolis, IN, USA) was administered intraperitoneally, and blood samples were drawn from the tail vein at 0, 30, 60, 90, and 120 min after administration. Plasma insulin levels were measured with an ELISA kit (Morinaga Institute of Biological Science, Kanagawa, Japan).
3
Measuring Urinary Adenosine Excretion
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Blood glucose levels were measured using a portable glucose meter (Antisense III; HORIBA, Ltd., Kyoto, Japan). Glycated hemoglobin (HbA1c) levels were measured using a DCA 2000 Analyzer (Siemens Medical Solutions Diagnostics, Tokyo, Japan) at the end of the experimental periods. Urine samples were collected using the metabolic cage. Urinary adenosine concentration was measured through the liquid chromatography‐tandem mass spectrometry method (Shimadzu Techno‐Research Inc., Kyoto, Japan), as described in the electronic Appendix S1 methods for details. Urinary creatinine levels were measured with a creatinine companion kit (Exocell Inc., Philadelphia, PA, USA). Urinary adenosine excretion is shown as the urinary adenosine : creatinine ratio.
4
Diabetic Kidney Disease: STZ-Induced Mouse Model
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Eight-week-old male CD-1 mice (Sankyo Laboratory Service, Tokyo, Japan) were used in all in vivo experiments. The mice were injected with STZ [200 mg/kg BW] intraperitoneally. The diagnosis of diabetes was confirmed by a blood glucose level >16 mmol/L 2 weeks after the STZ injection. At 4 weeks after the induction of diabetes, the diabetic mice were divided into two groups (TENE [30 mg/kg BW/day in drinking water] and untreated). TENE was diluted directly in the drinking water. Simultaneously, the mice received an FFA-bound BSA injection (0.3 g/30 g BW). BSA was purchased from Sigma-Aldrich (St. Louis, MO, USA). BSA was injected intraperitoneally for 11 days of 14 days, and subsequently, we observed tubular damage associated with inflammation, apoptosis and fibrosis.
All mice were sacrificed 2 weeks after the BSA injection and initiation of the TENE (Mitsubishi Tanabe Pharma, Tokyo, Japan) treatment. Before sacrificing the mice, their blood pressure was monitored via the tail cuff method using BP-98A (Softron Co., Beijing, China). The blood glucose levels were measured using a portable glucose meter (Antisense III, HORIBA, Ltd., Kyoto, Japan). All samples were collected and stored at −80 °C until use.
All mice were sacrificed 2 weeks after the BSA injection and initiation of the TENE (Mitsubishi Tanabe Pharma, Tokyo, Japan) treatment. Before sacrificing the mice, their blood pressure was monitored via the tail cuff method using BP-98A (Softron Co., Beijing, China). The blood glucose levels were measured using a portable glucose meter (Antisense III, HORIBA, Ltd., Kyoto, Japan). All samples were collected and stored at −80 °C until use.
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