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Erα ab32063

Manufactured by Abcam
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Sourced in United Kingdom, United States

ab32063 is an antibody that recognizes the Estrogen Receptor alpha (ERα) protein. It is a recombinant rabbit monoclonal antibody.

Automatically generated - may contain errors

Lab products found in correlation

H3k27ac ab4729, by Abcam (1 mentions) Normal rabbit igg sc 2027, by Santa Cruz Biotechnology (1 mentions) Normal mouse igg sc 2025, by Santa Cruz Biotechnology (1 mentions) H3k9me2 ab1220, by Abcam (1 mentions) H3k4me1 ab8895, by Abcam (1 mentions) H3k4me2 ab32356, by Abcam (1 mentions) H3k4me3 ab1012, by Abcam (1 mentions) Total h3 ab1791, by Abcam (1 mentions) Lsd1 ab17721, by Abcam (1 mentions) Erβ ab288, by Abcam (1 mentions) Vinculin ab18058, by Abcam (1 mentions) Gm6001, by MedChemExpress (1 mentions)

Close spelling variants of this product

Ab32063

2 protocols using erα ab32063

1

Chromatin Modification Profiling

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ERα ab32063 ((Abcam, Cambridge, UK); ERβ ab288 (Abcam); H3K4me1 ab8895 (Abcam); H3K4me2 ab32356 (Abcam); H3K4me3 ab1012 (Abcam); H3K9me2 ab1220 (Abcam); H3K9me3 ab8898 (Abcam); H3K27Ac ab4729 (Abcam); Total H3 ab1791 (Abcam); LSD1 ab17721 (Abcam); JMJD2A sc-271,210 (Santa Cruz Biotechnology); Normal rabbit IgG sc-2027 (Santa Cruz Biotechnology); Normal mouse IgG sc-2025 (Santa Cruz Biotechnology).
Romano A., Feola A., Porcellini A., Gigantino V., Di Bonito M., Di Mauro A., Caggiano R., Faraonio R, & Zuchegna C. (2020). Estrogen Induces Selective Transcription of Caveolin1 Variants in Human Breast Cancer through Estrogen Responsive Element-Dependent Mechanisms. International Journal of Molecular Sciences, 21(17), 5989.
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2

Antibody-based Analysis of Cell Signaling

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Antibodies and dilutions used were as follows: ERα (ab32063: immunohistochemistry, 1:150; immunoblotting, 1:750; immunofluorescence, 1:150) from Abcam; vinculin (ab18058: immunohistochemistry, 1:100; immunoblotting, 1:400; immunofluorescence, 1:150) from Abcam; CD68 (916104: immunohistochemistry, 1:100) from BioLegend; GAPDH (CW0101: immunoblotting, 1:1,000) from CWBIOTECH; p-MLC(3671: immunoblotting, 1:750; immunofluorescence, 1:50) from Cell Signaling Technology; MLC (10906-1-AP, immunoblotting, 1:500) from Proteintech; F-actin (40734ES75: immunofluorescence, 1:100) from YEASEN; fluorescein isothiocyanate (FITC)-conjugated anti-rabbit and Cy3-conjugated anti-mouse antibodies from ZHUANGZHIBIO.
The inhibitors are as follows: in vivo: GM6001 (MedChem Express, Monmouth Junction, NJ, USA) was subcutaneously injected every 3 days at 100 mg per kg body weight57 (link). In vitro: GM6001 (27 nm).
Gao Y., Wang Z., Hao Q., Li W., Xu Y., Zhang J., Zhang W., Wang S., Liu S., Li M., Xue X., Zhang W., Zhang C, & Zhang Y. (2017). Loss of ERα induces amoeboid-like migration of breast cancer cells by downregulating vinculin. Nature Communications, 8, 14483.
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