Human AML THP-1 cells were purchased from the Cell Bank of Chinese Academy of Sciences. Matrine was purchased from Tianyuan Biological Agent Plant. The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. RPMI-1640 medium, fetal bovine serum (FBS), dimethyl sulfoxide dissolving (DMSO), penicillin and streptomycin were purchased from Invitrogen; Thermo Fisher Scientific, Inc. The ELISA reader ELx800 was obtained from Bio-Tek Instruments, Inc. Inverted phase contrast microscope was obtained from Olympus Corporation. LY294002 was purchased from Cell Signaling Technology, Inc. and was dissolved in DMSO according to the manufacturer's instructions. Antibodies, including anti-PI3K, anti-phosphorylated (p)-PI3K, anti-mTOR, anti-p-mTOR, anti-Akt, anti-p-Akt and anti-β-actin were purchased from ABclonal Biotech Co., Ltd. The current study was approved by the Ethics Committee of Bengbu Medical College (Bengbu, China).
Anti mtor
Manufactured by ABclonal
Sourced in China, United States
Anti-mTOR is a laboratory reagent that targets the mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that regulates cell growth, proliferation, and survival. Anti-mTOR is used in research applications to study the role of mTOR in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Inverted phase contrast microscope, by Olympus (1 mentions)
Elx800 elisa reader, by Agilent Technologies (1 mentions)
Anti pi3k, by ABclonal (1 mentions)
Anti p akt, by ABclonal (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Anti akt, by ABclonal (1 mentions)
Anti β actin, by ABclonal (1 mentions)
Anti fgf3, by ABclonal (1 mentions)
Anti egfr, by ABclonal (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti beclin1, by ABclonal (1 mentions)
Anti caspase 3, by ABclonal (1 mentions)
Ipvh00010, by Merck Group (1 mentions)
Mini gel apparatus, by Bio-Rad (1 mentions)
3 protocols using anti mtor
1
Matrine Inhibits AML Cell Viability
2
Quantifying Protein Expression Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein was extracted and protein concentration was determined with the BCA Protein Assay Kit (Pierce, Rockford, IL, United States). Equivalent amounts of protein samples were uploaded and separated by SDS-PAGE and then electrotransferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corp, Atlanta, GA, US). The membranes were blocked in 5% non-fat dry milk powder at room temperature for 1 h, and then incubated overnight at 4°C with primary antibodies: anti-FGF3 (1:1000 dilution, Abclonal, Cat # A19052), anti-FGF4 (2 µg/ml, Abclonal, Cat # ab65974), anti-FGF19 (1:1000 dilution, Abcam, Cat # ab85042), anti-EGFR (1:1000 dilution, Abclonal, Cat # A11351), anti-phospho-EGFR-Y1068 pAb (1:1000 dilution, Abclonal, Cat # AP0301), anti-mTOR (1:1000 dilution, Abclonal, Cat # A11354), anti-phospho-mTOR (2448) (1:1000, Abcam, ab109268), anti-p44/42 MAPK (Erk1/2) (1:1000, CST, 4695S), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, CST, 4370S), anti-GAPDH (1:1000 dilution, CST, Cat#5174S), anti-beta-tubulin (1:1000 dilution, CST, Cat#2146). Membranes were then incubated with HRP-conjugated secondary antibodies at room temperature for 1 h. The signals of bands were detected by ECL reagents.
3
Western Blot Analysis of Autophagy and Cell Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues or harvested cells were homogenized and sonicated in RIPA (strong) protein extract buffer (Beyotime) containing a protease inhibitor and phosphatase inhibitor (Mannheim Roche, Germany). Equal amounts of protein were separated on SDS-polyacrylamide gels in a mini-gel apparatus (Bio-Rad, Hercules, CA, USA). Next, the protein bands were transferred onto polyvinylidene fluoride (Millipore, IPVH00010, Burlington, MA, USA) membranes that were blocked with 5% milk at room temperature for 1 h. The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-Beclin1 (Abclonal, A7353), anti-ATG3 (Abclonal, A5809), anti-Caspase-3 (Abclonal, A19654), anti-mTOR (Abclonal, A2445), anti-AMPK (Abclonal, A16960), anti-LC3-II&LC3-I (CST, #12741S), anti-p-AMPKα1 (CST, #2537S) or anti-β-actin-HRP (Bioworld, BS6007MH). After being washed 3 times, the membranes were incubated with goat anti-rabbit antibody (Abclonal, AS014) for 1 h at room temperature. Next, the membranes were washed again, and the immunostained protein bands were visualized by enhanced chemiluminescence (Thermo Scientific, 32106, Waltham, MA, USA). NoveVR 4. 6. 2 software (Bio-Rad) was to analyze the staining intensity of each band.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!