Tris 2 aminoethyl amine tren

Waxy potato starch (>95% amylopectin, molecular weight in the range 10⁷–10⁹ Da and roughly 5% of α (1–6) branching points) was kindly donated by Avebe (Veendam, The Netherlands) and dried under vacuum at 60 °C for 48 h before use. Lithium chloride was purchased from Sigma-Aldrich and dried under vacuum at 80 °C for 24 h before use. Anhydrous N,N-dimethylacetamide (DMAc) was purchased from Sigma-Aldrich in Sure/Seal™ (Steinheim, Germany). 2-bromopropionyl bromide (BpB), formaldehyde solution (37%), and formic acid (>95%) were purchased from Sigma-Aldrich and used as received. Tris(2-aminoethyl)amine (Tren) was purchased from Tokyo Chemical Industry Co., Ltd. (TCI, Tokyo, Japan) and used as received. Tris[2-(dimethylamino)ethyl]amine (Me₆Tren) was synthesized following the procedures reported [33 ]. N-IsopropylAcrylamide (NIPAM, stabilized with 4-Methoxyphenol (MEHQ)) was purchased from TCI and recrystallized from acetone to remove the inhibitor. Acrylamide (AM) was purchased from Sigma-Aldrich and used as received. Copper powder (<75 μm) was purchased from Sigma-Aldrich and stored under an N₂ atmosphere.

Waxy potato starch (>95% amylopectin) was kindly donated by Avebe (Veendam, The Netherlands) and dried under vacuum at 60 °C for 48 h before use. Lithium chloride was purchased from Sigma–Aldrich (Steinheim, Germany) and dried under vacuum at 80 °C for 24 h before use. Anhydrous N,N-dimethylacetamide (DMAc) was purchased from Sigma–Aldrich in Sure/Seal™ (Steinheim, Germany). 2-bromopropionic acid (BpA), 2-bromopropionyl bromide (BpB), formaldehyde solution (37%), and formic acid (>95%) were purchased from Sigma–Aldrich (Steinheim, Germany) and used as received. Tris(2-aminoethyl)amine (TREN) was purchased from Tokyo Chemical Industry Co., LTD (TCI, Tokyo, Japan) and used as received. Tris[2-(dimethylamino)ethyl]amine (Me₆TREN) was synthesized following the procedures reported [28 ] and [2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SBMA) (97%) was purchased from Merck (Darmstadt, Germany) and used as received. Copper(I) bromide (from Sigma-Aldrich) was stirred in sulfuric acid (from Sigma–Aldrich) solution for 2 h, then filtered, washed with ethanol and acetone respectively three times and dried under vacuum at room temperature for 24 h, followed by storage under N₂ atmosphere.