Emla cream 5% (lidocaine 25 mg/g, prilocaine 25 mg/g; Astrazeneca Korea, Seoul, Republic of Korea) was used as an anesthetic. A 30 G × 1/2 needle manufactured by the International Hongchim Association connected to an 80 mm folder was used for Jae-Seng Acupuncture treatment.
Prilocaine
Manufactured by AstraZeneca
Sourced in Sweden, United Kingdom
Prilocaine is a local anesthetic drug used in various medical and dental procedures. It works by temporarily blocking the transmission of pain signals from the affected area. Prilocaine is available in different formulations and concentrations for various clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using prilocaine
1
Topical Anesthetic for Acupuncture Procedure
2
Anesthesia and Dexmedetomidine in Pediatric Surgery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
No premedication was administered. A peripheral venous catheter was inserted approximately 2 h prior to surgery (while in the unit). EMLA cream (lidocaine 2.5% and prilocaine 2.5%, Astra Zeneca Inc., Sweden) was used to ease venous cannulation. HR, noninvasive arterial blood pressure, blood oxygen saturation (SaO2), electrocardiogram (ECG), and bispectral index (BIS) were monitored. After preoxygenation via face mask, anesthesia was induced with propofol 2–3 mg/kg and inhalation of 6 vol% sevoflurane. After the pupils were fixed, the laryngeal mask airway (LMA) was inserted and the ilioinguinal/iliohypogastric nerve block was performed by ultrasound guidance to relieve postoperative pain. Anesthesia was maintained with 2-3 vol% sevoflurane to maintained BIS from 40 to 60 and retaining spontaneous respiration. After the vital signs were stable, study groups received a rapid bolus injection of different doses of DEX (Jiangsu singch pharmaceutical co., LTD) at a rate of less than 5 s, while patients in the control group received saline in an equal volume. When the surgery was completed, every one of the patients was moved to the post-anesthesia care unit (PACU), and the children naturally regained consciousness.
3
Transient Focal Cerebral Ischemia Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transient focal cerebral ischaemia was induced by middle cerebral artery occlusion (MCAo) for 30 mins, based on our previously published protocol using the intraluminal filament model.9 (link)
Briefly, a midline incision was made on the ventral surface of the neck and the left common carotid artery isolated and ligated. Topical anaesthetic (EMLA, 5% prilocaine and lidocaine, AstraZeneca, UK) was applied to skin incision sites prior to incision. The internal carotid artery and the pterygopalatine artery were temporarily ligated. A 6-0 monofilament (Doccol, Sharon, MA, USA) was introduced into the internal carotid artery via an incision in the common carotid artery. The filament was advanced approximately 10 mm into the common carotid with the filament making its way distal to the carotid bifurcation, beyond the origin of the middle cerebral artery. A 10 mm mark was made on the filament to visualise the required length to be inserted beyond the carotid bifurcation. At 3 h from start of occlusion, animals were treated under isoflurane anaesthesia with vehicle (serum-free Mesenpro media), 9.1 × 104 IL-1α conditioned MSCs or 9.1 × 104 non-conditioned MSCs by intra-arterial infusion with a syringe driver (20 µl cell suspension, 0.5 µl/sec), via the filament incision site. Animals were recovered and returned to normal housing prior to behavioural testing.
Briefly, a midline incision was made on the ventral surface of the neck and the left common carotid artery isolated and ligated. Topical anaesthetic (EMLA, 5% prilocaine and lidocaine, AstraZeneca, UK) was applied to skin incision sites prior to incision. The internal carotid artery and the pterygopalatine artery were temporarily ligated. A 6-0 monofilament (Doccol, Sharon, MA, USA) was introduced into the internal carotid artery via an incision in the common carotid artery. The filament was advanced approximately 10 mm into the common carotid with the filament making its way distal to the carotid bifurcation, beyond the origin of the middle cerebral artery. A 10 mm mark was made on the filament to visualise the required length to be inserted beyond the carotid bifurcation. At 3 h from start of occlusion, animals were treated under isoflurane anaesthesia with vehicle (serum-free Mesenpro media), 9.1 × 104 IL-1α conditioned MSCs or 9.1 × 104 non-conditioned MSCs by intra-arterial infusion with a syringe driver (20 µl cell suspension, 0.5 µl/sec), via the filament incision site. Animals were recovered and returned to normal housing prior to behavioural testing.
4
Standardized Sevoflurane Anesthesia and Laparotomy in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sevoflurane exposure was performed according to a previously described protocol.
30 (link) Briefly, sevoflurane exposure and/or laparotomy was started at ZT0. Mice were exposed to 2% sevoflurane in 22% oxygen for 2 h in an anesthesia chamber; the concentration was monitored with a gas outlet. The heart rate, blood‐oxygen saturation, and rectal temperature were monitored. The mice breathed spontaneously, and sevoflurane was well tolerated, with all monitored variables in the physiological range.
Laparotomy was aseptically performed with a method previously used in mice.
31 (link) Mice were anesthetized for 2 h with 2% sevoflurane and intracutaneously injected with 0.2% ropivacaine along the planned incision line. A 2‐cm vertical incision was made in the middle of the abdomen, the gastrointestinal tract was exteriorized and vigorously rubbed for 30 s, and the organs (liver, spleen, kidneys, and bowel) were gently probed with cotton for 30 min. The intestines were then placed back into the peritoneal cavity, and the skin was sutured with surgical staples. EMLA cream (2.5% lidocaine and 2.5% prilocaine, AstraZeneca, Sweden) was applied to the incision wound at the end of surgery and then every 8 h for 2 days for surgical pain relief. Body temperature was maintained with a heating pad during anesthesia/surgery.
30 (link) Briefly, sevoflurane exposure and/or laparotomy was started at ZT0. Mice were exposed to 2% sevoflurane in 22% oxygen for 2 h in an anesthesia chamber; the concentration was monitored with a gas outlet. The heart rate, blood‐oxygen saturation, and rectal temperature were monitored. The mice breathed spontaneously, and sevoflurane was well tolerated, with all monitored variables in the physiological range.
Laparotomy was aseptically performed with a method previously used in mice.
31 (link) Mice were anesthetized for 2 h with 2% sevoflurane and intracutaneously injected with 0.2% ropivacaine along the planned incision line. A 2‐cm vertical incision was made in the middle of the abdomen, the gastrointestinal tract was exteriorized and vigorously rubbed for 30 s, and the organs (liver, spleen, kidneys, and bowel) were gently probed with cotton for 30 min. The intestines were then placed back into the peritoneal cavity, and the skin was sutured with surgical staples. EMLA cream (2.5% lidocaine and 2.5% prilocaine, AstraZeneca, Sweden) was applied to the incision wound at the end of surgery and then every 8 h for 2 days for surgical pain relief. Body temperature was maintained with a heating pad during anesthesia/surgery.
5
Standardized Sudomotor Wrinkling Test with EMLA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used a modified method of SSW-EMLA test described by Ng et al.[21 (link)] First, both hands of the patient were washed using soap and water and dried. The examiner observed the initial appearance of the fingers and photographed them before EMLA 5% cream (lidocaine 2.5% and prilocaine 2.5%; AstraZeneca; Cambridge, United Kingdom) application. A thick covering of EMLA 5% cream was applied to the distal segment of the 2nd, 3rd, and 4th fingers of both right and left hands. Each finger was wrapped in a food grade plastic wrap and sealed using skin bandage. After the application of EMLA cream, the patients were asked to wait for 30 minutes and to keep the hands dry and clean. The patients were not allowed to drink coffee, tea, or smoke cigarettes during the waiting period. If the EMLA application was compromised, the patient waited for an extra hour and the whole procedure was repeated.
When 30 minutes were up, the examiner opened the bandages and examined the skin for wrinkling. The finger-tip appearances were compared with a special scale (Fig.1 ) and scored. Wrinkling grades for digits 2, 3, and 4 were counted and average obtained. A difference of ≥3 points per hand (i.e., ≥1 point per digit) was taken as a cut-off or a different score. The SSW-EMLA test was considered abnormal if the total wrinkling score of each hand was <9.
When 30 minutes were up, the examiner opened the bandages and examined the skin for wrinkling. The finger-tip appearances were compared with a special scale (Fig.
6
Genioglossus EMG Electrode Placement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genioglossus EMG was recorded with fine-wire hook electrodes, inserted percutaneously using ultrasound to guide placement. To minimise any pain with wire insertion a topical anaesthetic (Emla cream 5% lignocaine and prilocaine, AstraZeneca, Australia) was applied under the chin 30 minutes prior to the ultrasound.
Participants lay supine, with the head positioned so that the mandible was perpendicular to the bed.
Submental anatomy including the mandible, mylohyoid, geniohyoid and genioglossus was visualised using an ultrasound device (iU22, Philips, Best, Netherlands) at 7 MHz transducer frequency.
The EMG electrodes were fashioned from single stranded stainless steel coated in Teflon (127 micron diameter wire, #791500 A-M Systems Inc, WA, USA). The wire was threaded onto a sterile hypodermic needle, the recording tip of the wire stripped bare of Teflon for 1.
Participants lay supine, with the head positioned so that the mandible was perpendicular to the bed.
Submental anatomy including the mandible, mylohyoid, geniohyoid and genioglossus was visualised using an ultrasound device (iU22, Philips, Best, Netherlands) at 7 MHz transducer frequency.
The EMG electrodes were fashioned from single stranded stainless steel coated in Teflon (127 micron diameter wire, #791500 A-M Systems Inc, WA, USA). The wire was threaded onto a sterile hypodermic needle, the recording tip of the wire stripped bare of Teflon for 1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!