Cd57 fitc clone tb03

Manufactured by Miltenyi Biotec

CD57-FITC (clone TB03) is a fluorochrome-conjugated monoclonal antibody used for the identification and enumeration of CD57+ cells in flow cytometric analysis. CD57 is a carbohydrate antigen expressed on a subset of T cells and natural killer cells.

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Lab products found in correlation

Cd56 apc, by Miltenyi Biotec (1 mentions) Cd107a pe cy5, by Thermo Fisher Scientific (1 mentions) Cd2 pe cy7, by Sony (1 mentions) Cd56 brilliant violet 421, by Sony (1 mentions) Hla dr pe, by Sony (1 mentions) Cd56 pe, by Agilent Technologies (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd56 pe cy7 clone ncam16.2, by BD (1 mentions) Cd3 percp clone sk7, by BD (1 mentions)

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CD57-FITC (2 citations)

2 protocols using cd57 fitc clone tb03

Multiparameter Flow Cytometry for NK Cells

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For surface and intracellular staining of NK cells the following mouse anti-human fluorescent-labeled Abs were used: CD2-PE-Cy7 (clone TS1/8), CD56-BrilliantViolet421 (clone HCD56), CD56-AF488 (clone 5.1H11), CD56-PE-Cy7 (clone 5.1H11), CD57-PE (clone TB01), HLA-DR-FITC (clone LN3), HLA-DR-PE (clone L243), IFN-gamma-FITC (clone 4S.B3), KI-67-PE (clone Ki-67), NKp46-FITC (clone 9E2) (Sony Biotechnology, USA), CD16-PE (Sorbent, Russia), CD16-APC (clone REA423), CD56-APC (clone REA196), CD57-FITC (clone TB03), CD57-APC (clone TB03), CD57-PE-Vio770 (clone TB03) (Miltenyi Biotec, Germany), CD44-F (clone J.173, Immunotech, USA), CD56-PE (clone C5.9, Dako, USA), CD107a-PE-Cy5 (clone H4A3), NKp30-PE (clone P30-15) (eBioscience, USA), CD161-AlexaFluor647 (clone HP-3G10), Granzyme B-Alexa Fluor 647 (clone GB11), KIR2DL2/DL3-PE (clone DX27) (Biolegend, USA), FcεRIγ-FITC (polyclonal, Milli-Mark, Merck, Germany), NKG2A-PE (clone 131411), NKG2C-AlexaFluor 488 (clone 108724), NKG2C-PE (clone 134591) (R&D Systems, USA). Supernatant of hybridoma producing Abs to human KIR2DL1 was kindly provided by prof. Miguel Lopez-Botet (Universitat Pompeu Fabra, Barcelona, Spain). Sheep anti-mouse IgG-FITC and PE (Sigma-Aldrich, USA) were used as second Abs for anti-KIR2DL1. IgG1-PE (clone IS5-21F5, Miltenyi Biotec, Germany) was used as isotype control for NKG2C-PE Ab staining.

Kobyzeva P.A., Streltsova M.A., Erokhina S.A., Kanevskiy L.M., Telford W.G., Sapozhnikov A.M, & Kovalenko E.I. (2020). CD56dimCD57−NKG2C+ NK cells retaining proliferative potential are possible precursors of CD57+NKG2C+ memory-like NK cells. Journal of leukocyte biology, 108(4), 1379-1395.

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CD57+ Cell Depletion and Flow Analysis

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Depletion of CD57 + cells was performed per manufacturer's protocol using human anti-CD57 MicroBeads (Miltenyi Biotec). The efficacy of the depletion was assessed by flow cytometry using CD3 PerCP (clone SK7; BD Biosciences), CD56 PE-Cy7 (clone NCAM16.2; BD Biosciences) and CD57 FITC (clone TB03; Miltenyi Biotec) antibodies. Cells were acquired on an LSR Fortessa flow cytometer (BD Biosciences) and data were analyzed using FlowJo software (FlowJo LLC).

Kristensen A.B., Wragg K.M., Vanderven H.A., Lee W.S., Silvers J., Kent H.E., Grant M.D., Kelleher A.D., Juno J.A., Kent S.J, & Parsons M.S. (2022). Phenotypic and functional characteristics of highly differentiated CD57+NKG2C+ NK cells in HIV-1-infected individuals. Clinical and experimental immunology, 210(2).

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