As previously reported, the immunofluorescence (IF ) experiment procedure was carried out (Liu et al., 2018a ). The primary antibodies used in the IF experiment were as follows: mouse anti-BrdU monoclonal antibody (1:200, G3G4, DSHB, IA), rabbit anti-PCNA antibody (1: 100, bs-0754R, Bioss, Beijing, China). The secondary antibodies used in the IF experiment were as follows: goat anti-mouse IgG (H + L)-TRITC (1:100, BS11502, Bioworld Technology, Inc., Minneapolis, MO), goat anti-rabbit antibody (1:50, HA1016, HuaBio, Hangzhou, China). After that, the IX70 fluorescence microscope (Olympus, Tokyo, Japan) was used to observe the fluorescence image.
Rabbit anti pcna antibody
Manufactured by Bioss Antibodies
The Rabbit anti-PCNA antibody is a primary antibody that specifically recognizes the Proliferating Cell Nuclear Antigen (PCNA) protein. PCNA is a nuclear protein that functions as a cofactor for DNA polymerase delta and plays a crucial role in cellular DNA replication and repair processes.
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2 protocols using rabbit anti pcna antibody
1
Immunofluorescence Staining for BrdU and PCNA
2
Protein Expression Analysis in Boar Testes
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Protein (20 μg) from the preweaning, cryptorchid, and contrascrotal boar testes were separated using SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were blocked with blocking buffer (5% non-fat dried milk) for 30 min at room temperature, and then incubated overnight at 4 °C in blocking buffer containing polyclonal rabbit anti-PCNA antibody (1:300, Bioss, bs-2007R), polyclonal rabbit anti-LC3 antibody (1:200, Bioss, bs-8878R), and polyclonal rabbit anti-GAPDH antibody (1:2, 500, Abcam, ab9485), respectively. The next day, membranes were incubated in polymerized HRP-conjugated goat anti-rabbit secondary antibody (1:12,000) (CWBIO, Beijing, China) at 37 °C for 1 h and then washed three times with TBST. Specific protein bands were visualized and quantified using the ECL method and ImageJ software (National Institutes of Health, USA). Protein levels were corrected for GAPDH expression levels. All experiments were performed in triplicate.
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