Factor four vf 1 capillary column

The analyses of the EOs were performed by a gas chromatograph with a flame ionization detector (FID) and coupled to a mass spectrometer (MS) Perkin Elmer Clarus 500 model (Waltham, MA, USA). The oven GC was equipped with a Varian Factor Four VF-1 capillary column and helium was used as carrier gas at a flow rate of 1 mL/min. The operative conditions followed [47 (link)] with some modifications.
Mass spectra were taken at 70 eV (EI) with a mass scan range of 40–500 m/z. The identification of constituents was obtained by matching their mass spectra with those stored in the Wiley and NIST 02 mass spectra libraries database. Furthermore, the Linear Retention Indices (LRIs), with reference to the series of C8–C30 aliphatic hydrocarbons, were calculated and compared with available retention data found in the literature. Relative amounts were expressed as percentages obtained by peak area normalization from GC-FID chromatograms without the use of an internal standard or correction factors. All analyses were performed three times.

To describe the volatile chemical profile of the two Eos, a Clarus 500 model Perkin Elmer (Waltham, MA, USA) gas chromatograph coupled with a mass spectrometer and equipped with a flame detector ionization (FID), was used. The separation of compounds was performed by a Varian Factor Four VF-1 capillary column [100 (link),101 (link)]. The oven temperature program started from 60 °C up to 220 °C for 20 min at a rate of 6 °C min⁻¹. Helium was the carrier gas at flow rate of 1.0 mL min⁻¹ in constant mode. For MS, the mass spectra were obtained in the electron impact mode (EI), at 70 eV in full-scan mode in the range 35–450 m/z. The compounds were identified by the matching their mass spectra with databases Wiley 2.2 (Wiley, NY, USA) and Nist 02 (Gaithersburg, MD, USA) and by comparing their linear retention indices (LRIs), relative to C₈–C₂₅ n-alkanes analyzed under the same conditions, with those available in the literature. The relative average percentages were calculated with respect to the total area of the chromatogram by normalizing the peak area without the use of an internal standard and any factor correction. All analyses were performed in triplicate.

To describe the volatile chemical profile of the two Eos, a Clarus 500 model Perkin Elmer (Waltham, MA, USA) gas chromatograph coupled with a mass spectrometer and equipped with a flame detector ionization (FID), was used. The separation of compounds was performed by a Varian Factor Four VF-1 capillary column [100 (link),101 (link)]. The oven temperature program started from 60 °C up to 220 °C for 20 min at a rate of 6 °C min⁻¹. Helium was the carrier gas at flow rate of 1.0 mL min⁻¹ in constant mode. For MS, the mass spectra were obtained in the electron impact mode (EI), at 70 eV in full-scan mode in the range 35–450 m/z. The compounds were identified by the matching their mass spectra with databases Wiley 2.2 (Wiley, NY, USA) and Nist 02 (Gaithersburg, MD, USA) and by comparing their linear retention indices (LRIs), relative to C₈–C₂₅ n-alkanes analyzed under the same conditions, with those available in the literature. The relative average percentages were calculated with respect to the total area of the chromatogram by normalizing the peak area without the use of an internal standard and any factor correction. All analyses were performed in triplicate.