Xcelligence rtca mp system

Manufactured by Agilent Technologies

Sourced in United States

The XCELLigence RTCA MP System is a real-time cell analysis platform developed by Agilent Technologies. It enables continuous monitoring and quantification of cell proliferation, cytotoxicity, and cell migration in a label-free manner.

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Lab products found in correlation

E plate 96, by Agilent Technologies (2 mentions) 16 well e plate, by Agilent Technologies (2 mentions) Prism 7, by GraphPad (2 mentions) Rtca software 2, by Agilent Technologies (1 mentions)

5 protocols using xcelligence rtca mp system

Real-Time Cell Proliferation Assay of H-1PV Infection

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Cell proliferation was monitored in real time using the RTCA-MP xCELLigence system (ACEA Biosciences Inc., San Diego, CA, USA) including 6 × 96-well electronic microtiter plate modules. Cells were seeded on a 96-well E-Plate at a density of 4000–16000 cells/well (according to cell doubling rate). Cells were infected 24–72 h later with increasing amounts of wild-type H-1PV, ranging from MOI 0.05 pfu/cell to 50 pfu/cell (0, 0.05, 0.25, 0.5, 1, 5, 10 and 50). Growth of untreated and H-1PV-infected cells was monitored in real time for 5–7 days every 30 min and expressed as normalised cell index (CI), a parameter proportional to the number of attached cells per well and, therefore, strictly correlated with cell proliferation rate. The experiment was carried out at least three times for every condition. Six leukaemia cancer cell lines that grow in suspension were excluded from the screening, because of incompatibility with the xCELLigence system, which monitors the growth of only adherent-growing cell lines.

Kulkarni A., Ferreira T., Bretscher C., Grewenig A., El-Andaloussi N., Bonifati S., Marttila T., Palissot V., Hossain J.A., Azuaje F., Miletic H., Ystaas L.A., Golebiewska A., Niclou S.P., Roeth R., Niesler B., Weiss A., Brino L, & Marchini A. (2021). Oncolytic H-1 parvovirus binds to sialic acid on laminins for cell attachment and entry. Nature Communications, 12, 3834.

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Real-Time Monitoring of Cell Proliferation

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Experiments conducted on the RTCA-MP xCELLigence system (ACEA Biosciences, San Diego, CA) were performed in accordance with the instructions of the supplier. Complete growth medium (100 µL) was added into each well of the E-plate 96 (ACEA Biosciences), followed by a brief background impedance measurement on the RTCA-MP station. MCF10A and MCF10A CDH1 -/-cells were seeded at 4×10 3 cells per well in 100 µL complete growth medium, and, after 30 min equilibration at room temperature, the E-plate was placed in the RTCA-MP station. The RTCA-MP station was housed in a humidified cell culture incubator at 37 °C and 5% CO 2 . Cell proliferation, as determined by electrical impedance, was recorded at 15-min intervals. After overnight incubation, the assay was paused, 10 µL medium was removed from each well, and cells were treated with 10 µL drug or 0.1% DMSO for controls. The assay was then resumed, taking impedance measurements every 15 min for a further 48 h. All xCELLigence experiments were performed in duplicate.

Single A., Beetham H., Telford B.J., Guilford P, & Chen A. (2015). A Comparison of Real-Time and Endpoint Cell Viability Assays for Improved Synthetic Lethal Drug Validation. Journal of biomolecular screening, 20(10).

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Real-Time Monitoring of Cell Proliferation

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Cell proliferation was monitored by the xCELLigence RTCA MP System (ACEA Biosciences, San Diego, CA, USA) using 16-well E-Plates (ACEA Biosciences). The cells were seeded in triplicate at 5 × 10³ cells/well in the plates. For the RTCA experiments, the cells were treated with puerarin after reaching steady growth (24 h). Impedance was measured every 15 min over 96 h and represented as the cell index by the RTCA-integrated software of the xCELLigence System. The cell index was normalized to 1 at the time point of drug administration. From this data, real-time cell growth curves were generated with GraphPad Prism 7 (GraphPad Software, La Jolla, CA, USA).

Zhu H., Xiao Y., Guo H., Guo Y., Huang Y., Shan Y., Bai Y., Lin X, & Lu H. (2021). The isoflavone puerarin exerts anti-tumor activity in pancreatic ductal adenocarcinoma by suppressing mTOR-mediated glucose metabolism. Aging (Albany NY), 13(23), 25089-25105.

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Monorhamnolipid Cytotoxicity Assessment

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H1299 cells were plated at a density of 2000 cells per well in a volume of 120 μL per well in an E-Plate 96 (Acea Biosciences, San Diego, CA). All plates were analyzed in an xCELLigence RTCA MP system (Acea BioSciences), while being maintained in a cell culture incubator at 37 °C and 5% CO₂. After plating, cells were cultured for 24 h, with measurements taken at 15 min intervals to measure Cell Index, a measure of electrical impedance across well electrodes resulting from cell adherence. Cells were then administered serial dilutions (0.002–1984 μM) of respective monorhamnolipid in a volume of 30 μL per well, with 8 replicate wells per dose. Plates were returned to the xCELLigence and the cytotoxicity of the monorhamnolipid was evaluated over 72 h with measurements taken at 15 min intervals. Inhibitory concentration 50, IC₅₀, values were determined using the DRC (area-under-curve in a time period vs concentration) function of the RTCA Software 2.0 (Acea Biosciences). Analysis was performed based on the mean value of the replicate wells. The timeframe chosen for analysis in each experiment was between the first sweep taken following addition of compounds and the last sweep of each assay. All compounds were tested with 3 biological replicates, unless otherwise indicated.

Hogan D.E., Tian F., Malm S.W., Olivares C., Pacheco R.P., Simonich M.T., Hunjan A.S., Tanguay R.L., Klimecki W.T., Polt R., Pemberton J.E., Curry J.E, & Maier R.M. (2018). Biodegradability and toxicity of monorhamnolipid biosurfactant diastereomers. Journal of hazardous materials, 364, 600-607.

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Real-time Cell Proliferation Monitoring

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Cell proliferation was monitored by the xCELLigence RTCA MP System (ACEA Biosciences, San Diego, CA, USA) using 16-well E-Plates (ACEA Biosciences). The cells were seeded in triplicate at 5 × 10 3 cells/well in the plates. For the RTCA experiments, the cells were treated with puerarin after reaching steady growth (24 h). Impedance was measured every 15 min over 96 h and represented as the cell index by the RTCAintegrated software of the xCELLigence System. The cell index was normalized to 1 at the time point of drug administration. From this data, real-time cell growth curves were generated with GraphPad Prism 7 (GraphPad Software, La Jolla, CA, USA).

Zhu H., Lü H., Xiao Y., Guo H., Guo Y., Huang Y., Liu X., Shan Y., Bai Y., & Lin X. (2021). The Isoflavone Puerarin Exerts Anti-Tumor Activitiy in Pancreatic Ductal Adenocarcinoma by Suppressing Akt/mTOR Activity.

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