All mice were fed a defined AIN-93M Mature Rodent diet (Research Diets) for a minimum of seven days to minimize feed-related autofluorescence in the gastrointestinal system [49 (link)]. B6 or B6FVB TRAMP (+) animals were injected either intraperitoneally with 1 × 106 CRC2631 pRSTmCherry or VNP20009 pRSTmCherry, or injected intravenously (tail vein injection) with 1 × 107 or 2.5 × 107 CRC2631iRFP720-cat. Fluorescent imaging was performed using a Xenogen IVIS 200 in vivo fluorescence system (Perkin-Elmer) and analyzed using Living Image software (v4.7.3). iRFP720 expression spectral unmixing was performed as previously described to detect CRC2631iRFP720-cat associated iRFP720 in vivo [32 (link)]. Images containing mCherry RFP (Supplementary Figure 3) were spectrally unmixed to distinguish the CRC2631 or VNP20009-associated signal in vivo using mCherry spectral unmixing settings in the Living Image software.
Xenogen ivis 200 in vivo fluorescence system
Manufactured by PerkinElmer
The Xenogen IVIS 200 is an in vivo fluorescence imaging system. It is designed to detect and quantify fluorescent signals from small laboratory animals.
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Lab products found in correlation
2 protocols using xenogen ivis 200 in vivo fluorescence system
1
In Vivo Fluorescent Imaging of Tumor Cells
2
Fluorescent Imaging of Colorectal Cancer
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All mice were fed a defined AIN-93M Mature Rodent diet (Research Diets) for a minimum of seven days to minimize feed-related autofluorescence in the gastrointestinal system [49] (link). B6 or B6FVB TRAMP(+) animals were injected either intraperitoneally with 1x106 CRC2631 pRSTmCherry or VNP20009 pRSTmCherry, or injected intravenously (tail vein injection) with 1x107 or 2.5x107 CRC2631iRFP720-cat. Fluorescent imaging was performed using a Xenogen IVIS 200 in vivo fluorescence system (Perkin-Elmer) and analyzed using Living Image software (v4.7.3). iRFP720 expression spectral unmixing was performed as previously described to detect CRC2631iRFP720-cat associated iRFP720 in vivo [32] (link). Images containing mCherry RFP (Supplemental Figure 3) were spectrally unmixed to distinguish the CRC2631 or VNP20009associated signal in vivo using mCherry spectral unmixing settings in the Living Image software.
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