Concentrations of IL-6 and IL-8 in cell-culture supernatants were measured using IL-6 and IL-8 DuoSet ELISA kits (R&D Systems Europe, Abingdon, UK) according to the manufacturer’s instructions.33 (link) The limits of detection for the IL-6 and IL-8 ELISA kits were 9.38 and 31.3 pg/mL, respectively. Levels of IL-6 and IL-6-inducible CXCL1 (KC) in BALF were measured using commercial mouse IL-6 and KC ELISA kits (R&D Systems Europe, Abingdon, UK). The limits of detection for the mouse IL-6 and KC ELISA kits were 1.8 and 2.0 pg/mL, respectively.
Il 8 duoset elisa kit
Manufactured by R&D Systems
Sourced in United Kingdom
The IL-8 DuoSet ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-8 (IL-8) levels in cell culture supernatants, serum, and plasma.
Automatically generated - may contain errors
5 protocols using il 8 duoset elisa kit
1
Quantifying IL-6 and IL-8 in Cell Cultures
2
Quantification of IL-8 Cytokine Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytokine concentrations were measured from aliquots of cell-free culture supernatants using commercially available IL-8 ELISA kits from Sanquin (Amsterdam, The Netherlands) for the cell lines (assay range = 1-240 pg/ml), and IL-8 duoset ELISA kits from R& D Systems Europe (Abingdon, UK) for NECs (assay range = 31.3-2000 pg/ml).
3
Measuring IL-6 and IL-8 in Chondrocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The levels of IL-6 and IL-8 in culture supernatants from primary and immortalized articular chondrocytes after 24-hour treatment with IL-1β were determined with the human IL-6 and IL-8 DuoSet ELISA Kit (DY206 and DY208, respectively; Bio-Techne R&D Systems, UK), following the manufacturer's instructions. Absorbance measurements were performed using a NanoQuant Infinite M200 microplate reader (Tecan Ibérica Instrumentación, Spain). Data were expressed as released picograms per ml of culture supernatant. Working range was between 9.38 to 600 pg/ml for IL-6 and 31.25 to 2,000 pg/ml for IL-8.
4
Quantification of Secreted IL-8 by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Secreted IL-8 protein levels were quantified with IL-8 DuoSet ELISA Kit (R&D Systems, Minneapolis, MS, USA) according to the manufacturer’s instructions. Supernatants were collected after 72 h of transfection, centrifuged and stored at − 20 °C until use. 3,3′,5,5′-Tetramethylbenzidine (TMB, Sigma-Aldrich) was used as visualizing reagent, substrate reaction was stopped with 1 M H2SO4. Optical density was measured with Epoch microplate spectrophotometer (BioTek, Winooski, VT, USA) at the wavelength of 450 nm.
5
Quantification of IL-6 and IL-8 Secretion
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!