Anti tgfβ clone 1d11.16.8

Manufactured by BioXCell

Anti-TGFβ (clone 1D11.16.8) is a monoclonal antibody that specifically binds to transforming growth factor-beta (TGF-β). It is designed for research applications.

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Lab products found in correlation

Nctc 109, by Thermo Fisher Scientific (1 mentions) Ultra low igg fbs, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Female nod ltj mice, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Clone 1D11.16.8 (8 citations) Anti-TGFβ antibody (clone 1D11.16.8; (5 citations) Anti-TGFβ (clone 1D11, (4 citations) TGFβ (1D11), (2 citations)

2 protocols using anti tgfβ clone 1d11.16.8

Generation and Purification of Antibodies

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Purified anti-CTLA-4 (clone 9D9), mouse IgG2b isotype (clone MPC-11) and anti-TGFβ (clone 1D11.16.8) were obtained from BioXCell. Anti-B7x was generated in-house from hybridoma culture. Hybridoma cells from clone 1H3 were cultured in Wheaton CELLine 350 flask. The cell compartment media was DMEM (Corning) supplemented with 10% ultra-low IgG FBS (Thermofisher), 10% NCTC-109 (Thermofisher), 1% non-essential amino acids, and 1% penicillin-streptomycin. The medium compartment media contained DMEM (Corning) supplemented with 1% penicillin-streptomycin. Antibodies were purified from cell culture supernatant by Protein G resin pulldown (Genscript). The purity and identity of antibodies were confirmed by SDS/PAGE.

John P., Pulanco M.C., Galbo PM J.r., Wei Y., Ohaegbulam K.C., Zheng D, & Zang X. (2022). The immune checkpoint B7x expands tumor-infiltrating Tregs and promotes resistance to anti-CTLA-4 therapy. Nature Communications, 13, 2506.

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Modulating TGFβ in NOD Mice

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Female NOD/LtJ mice (The Jackson Laboratory) were maintained at the Uniformed Services University (USU) animal facility with free access to food and water. All experiments were performed under protocols approved by the USU Institutional Animal Care and Use Committee. One hundred micrograms of aCeAg was administered in 100 μl of PBS by intraperitoneal (I.P.) injection starting at 8 weeks of age and continued twice weekly for 14 weeks.
For analysis of NOD mice with depleted circulating TGFβ, in addition to receiving bi-weekly aCeAg injections, mice received 100 μg anti–TGFβ (clone 1D11.16.8; Bio X Cell, catalog no. BE0087) or 100 μg mouse IgG1 isotype (clone MOPC-21; Bio X Cell, catalog no. BE0083) via I.P. injection 3x per week through studies end.

Jackson-Thompson B.M., Torrero M., Mitre B.K., Long J., Packiam M, & Mitre E. (2020). Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice. Journal of Translational Autoimmunity, 3, 100065.

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