Log In Sign up
The largest database of trusted experimental protocols

Lipofectamine 2000

Manufactured by Bio-Rad
Visit Supplier

Lipofectamine 2000 is a cationic lipid-based transfection reagent used for the delivery of genetic material, such as DNA or RNA, into mammalian cells. It facilitates the uptake of nucleic acids by forming lipid-DNA or lipid-RNA complexes that can be efficiently internalized by cells.

Automatically generated - may contain errors

Lab products found in correlation

Bep neon transfection system, by Thermo Fisher Scientific (2 mentions) Gene gun pds 1000 he system, by Bio-Rad (2 mentions)

2 protocols using lipofectamine 2000

1

Optimized Cellular Transfection via Nanochannels

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CNP, a single layer of MEFs, MSCs, DCs, or HEK- 293T cells (~200,000 cells) was spread on a 1 cm x 1 cm 3D CNP silicon chip surface for overnight cell incubation. Plasmids pre-loaded in PBS buffer were injected into individual cells via nanochannels (~500 nm diameter and 5 μm spacing) using a 200 V electric field for 5 pulses at 10 ms/pulse with a 0.1 second interval. Various electroporation conditions were tested for best choice. BEP (Neon Transfection System, Thermo Fisher Scientific), Gene Gun (PDS-1000/He™ System, Bio-Rad) and Lipofectamine 2000 transfections were conducted according to manufacturers’ instructions. Ascl1/Brn2/Myt1l plasmids at a weight ratio of 2/1/1 and a concentration of 100 ng/mL in PBS buffer, according to the protocol in literature,40 were pre-mixed for transfection. Cell transfection of PTEN, miR-128, CD47, CDX-CD47, CREKA-CD47 and CD63-GFP plasmids followed the same procedure.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
+ Open protocol
+ Expand
2

Optimized Cellular Transfection via Nanochannels

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CNP, a single layer of MEFs, MSCs, DCs, or HEK- 293T cells (~200,000 cells) was spread on a 1 cm x 1 cm 3D CNP silicon chip surface for overnight cell incubation. Plasmids pre-loaded in PBS buffer were injected into individual cells via nanochannels (~500 nm diameter and 5 μm spacing) using a 200 V electric field for 5 pulses at 10 ms/pulse with a 0.1 second interval. Various electroporation conditions were tested for best choice. BEP (Neon Transfection System, Thermo Fisher Scientific), Gene Gun (PDS-1000/He™ System, Bio-Rad) and Lipofectamine 2000 transfections were conducted according to manufacturers’ instructions. Ascl1/Brn2/Myt1l plasmids at a weight ratio of 2/1/1 and a concentration of 100 ng/mL in PBS buffer, according to the protocol in literature,40 were pre-mixed for transfection. Cell transfection of PTEN, miR-128, CD47, CDX-CD47, CREKA-CD47 and CD63-GFP plasmids followed the same procedure.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!