The generation of ROS by P8 in HeLa cells was monitored using the Reactive Oxygen Species Assay Kit (CA1410, Solarbio).[30 (link)
] HeLa cells were seeded into 96‐well plate and cultured at 37 °C, 5% CO2 atmosphere. After reaching 80% cell density, the cells were treated with P8 (5.0 µm ) for 6 h. The reactive oxygen species assay was performed following the provided instructions of the commercial assay kit. To induce intracellular ROS production, the cells were exposed to white light (3 mW·cm−2) for 15 min. Fluorescence images were collected using Olympus IX73 Research Inverted Microscope. The cells were excited using the blue light channel (460–490 nm), and emission images were collected using the green light channel (500–530 nm).
] HeLa cells were seeded into 96‐well plate and cultured at 37 °C, 5% CO2 atmosphere. After reaching 80% cell density, the cells were treated with P8 (5.0 µ