W plan apochromat 20 1.0 water immersion objective

We obtained the depth profiles (Fig. 2, B and C) of fluorescence intensity of acceptor dye emission $(580\pm 10\mathrm{nm})$ on the adaxial and abaxial leaf sides of maize leaves with a laser scanning confocal microscope (Zeiss LSM880). An intact maize leaf infiltrated with AquaDust was mounted on a glass slide using a double-sided tape, placed on Zeiss LSM880 Confocal Upright Microscope, and imaged using W Plan-Apochromat 20×/1.0 water immersion objective and a $561\mathrm{nm}$ excitation laser line. These depth profiles were obtained by constructing orthogonal sections and plotting intensity as a function of depth in these orthogonal sections using ZEN software and MATLAB. See Supplementary Methods S4 for details on sample preparation for cryogenic scanning electron micrographs in Fig. 2A.

In the experiments of laser-induced thrombosis, and visualization of fibrin net, a fixed-stage microscope Zeiss Axio Examiner Z1 (Carl Zeiss Microscopy GmbH, Germany), a confocal scanner unit (CSU-X1, Yokogawa Electric Corporation, Japan), and a W Plan-Apochromat 20 × /1.0 water immersion objective (Carl Zeiss Microscopy GmbH) were used. SlideBook 6.0 (Intelligent Imaging Innovations, Inc., United States)¹⁹ was used to analyze the recordings and images.

In the experiments involving laser-induced thrombosis and visualization of the fibrin net, we used specific equipment. The setup included a fixed-stage microscope, the Zeiss Axio Examiner Z1 (Carl Zeiss Microscopy GmbH, Oberkochen, Germany). Additionally, we utilized a confocal scanner unit (CSU-X1, Yokogawa Electric Corporation, Tokyo, Japan). The microscope was equipped with a W Plan-Apochromat 20×/1.0 water immersion objective (Carl Zeiss Microscopy GmbH, Oberkochen, Germany). For visualization purposes, we employed a confocal microscopy system to observe the thrombi formed in the flow chamber and the fibrin net in the clots. SlideBook 6.0 (Intelligent Imaging Innovations, Inc., Denver, CO, USA) was used to analyze the images from confocal microscopy.