The AMU‐ML2, SU‐DHL‐10, Raji, P3HR‐1, VAL, and Farage B‐cell lymphoma cell lines were cultured as previously described 22 . Prednisolone, cyclophosphamide, vincristine, and doxorubicin were purchased from Wako Pure Chemical Industries (Osaka, Japan).
Vincristine
Manufactured by Fujifilm
Sourced in Japan
Vincristine is a laboratory product manufactured by Fujifilm. It is a chemical compound used in various research and analytical applications. The core function of Vincristine is to serve as a standard or reagent in scientific experiments and procedures.
Automatically generated - may contain errors
Lab products found in correlation
Cyclophosphamide, by Fujifilm (2 mentions)
Etoposide, by Fujifilm (2 mentions)
Doxorubicin, by Fujifilm (1 mentions)
Prednisolone, by Fujifilm (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Sirnas, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
5 aza 2 deoxycytidine, by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
Brefeldin a, by BioLegend (1 mentions)
Pd153035, by Selleck Chemicals (1 mentions)
Lgbit, by Promega (1 mentions)
Lytic buffer, by Promega (1 mentions)
Colchicine, by Fujifilm (1 mentions)
Thapsigargin, by Fujifilm (1 mentions)
Ml240, by Merck Group (1 mentions)
96 well plate, by Corning (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Ab120003, by Abcam (1 mentions)
5 protocols using vincristine
1
Cell Culture and Compound Preparation
2
Evaluating p38 MAPK Inhibitors in Cell Culture
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We used the hydrochloride form of MX2 (Watanabe et al. 1988 , 1991 ). ADR, etoposide, vincristine, and dimethyl sulfoxide, were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Phosphate‐buffered saline without metal salt solution (PBS (−)) was purchased from Nissui (Tokyo, Japan). RPMI 1640, Hanks' balanced salt solution without Ca2+ or Mg2+ (HBSS), fetal calf serum, and gentamicin were purchased from Life Technologies, Inc. (Gaithersburg, MD). 5‐Aza‐2′‐deoxycytidine was purchased from Sigma Aldrich Japan (Tokyo, Japan). SB203580 (4‐(4‐fluorophenyl)‐2‐(4‐methylsulfinylphenyl)‐5‐(4‐pyridyl)1H‐imidazole) and SB202190 (4‐(4‐fluorophenyl)‐2‐(4‐hydroxyphenyl)‐5‐(4‐pyridyl)1H‐imidazole), which are p38 MAPK inhibitors, and SB202474 (4‐Ethyl‐2(p‐methoxyphenyl)‐5‐(4′‐pyridyl)‐IH‐imidazole), which is a negative control, were purchased from Calbiochem (Tokyo, Japan). siRNAs were obtained from Ambion (Carlsbad, CA).
3
Cytotoxicity Screening of Drug Candidates
4
Cell Viability Assay with Cytotoxic Drugs
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To assess cell viability, the sorted cells were plated at 5×103 cells per 96-well plates (Corning, Corning, NY, USA) for one day and then incubated with various concentrations of vincristine, cyclophosphamide and etoposide (Wako, Osaka, Japan) for three days. Subsequently, the degree of cell viability was investigated using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturer’s protocol.
5
Electrophysiological Profiling of Neuronal Cultures
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For hiPSC‐derived cortical neuron samples, spontaneous activities were recorded before treatment and after the cumulative addition to the culture medium of one of the following compounds: 4‐AP (10 × 10‐6 and 30 × 10‐6m ; 016‐02781, Wako), picrotoxin (1 × 10‐6 and 10 × 10‐6m ; 2800471, Nacalai tesque), and AP‐5 (25 × 10‐6m ; ab120 003, Abcam) followed by CNQX (30 × 10‐6m ; 032‐23121, Wako). All chemicals were dissolved in DMSO (0.1%), used as a vehicle.
For DRG samples, spontaneous activities were recorded before and after the cumulative addition of capsaicin (030‐11353, Wako) at 1 × 10‐9, 10 × 10‐9, and 100 × 10‐9m to the culture medium. Then the medium was replaced with a culture medium containing 10 × 10‐9m paclitaxel (161‐28164, Wako). After 24 h culture, spontaneous activities were rerecorded before and after capsaicin treatment.
For conduction velocity analysis experiments using DRG samples, spontaneous activities were recorded before, 2 h after, and 24 h after adding 3 × 10‐9m vincristine (220‐02301, Wako) to the culture medium.
In the recordings and drug administration, the cultures were kept at 37 °C under a 5% CO2 atmosphere.
For DRG samples, spontaneous activities were recorded before and after the cumulative addition of capsaicin (030‐11353, Wako) at 1 × 10‐9, 10 × 10‐9, and 100 × 10‐9
For conduction velocity analysis experiments using DRG samples, spontaneous activities were recorded before, 2 h after, and 24 h after adding 3 × 10‐9
In the recordings and drug administration, the cultures were kept at 37 °C under a 5% CO2 atmosphere.
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