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Elisa max kit

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ELISA MAX kits are a set of reagents designed to perform enzyme-linked immunosorbent assays (ELISAs) for the detection and quantification of specific proteins or other analytes in biological samples. The kits include the necessary components to conduct ELISA experiments, such as capture and detection antibodies, standards, and buffers. These kits provide a standardized and convenient solution for ELISA-based analyses.

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Lab products found in correlation

Duoset elisa kit, by R&D Systems (3 mentions) Cobas taqman, by Roche (2 mentions) Spark 10m, by Tecan (2 mentions) Synergy h1, by Agilent Technologies (2 mentions) Microplate reader, by Agilent Technologies (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Mouse tgfβ1 duoset elisa kit, by R&D Systems (1 mentions) Infinite 200 pro, by Tecan (1 mentions) Prism 5, by GraphPad (1 mentions) Aldesleukin, by Thermo Fisher Scientific (1 mentions) Mouse il 1β elisa kit, by Thermo Fisher Scientific (1 mentions) Facscallibur flow cytometer, by BD (1 mentions) Prism 7, by GraphPad (1 mentions) Bead based multiplex immunoassay, by Eve Technologies (1 mentions) 24 well plate, by Corning (1 mentions) Spectramax m2, by Molecular Devices (1 mentions) Avidin hrp, by BioLegend (1 mentions) Tmb substrate, by Merck Group (1 mentions) Legendplex system, by BioLegend (1 mentions) Nunc maxisorp 96 well elisa plate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ELISAs (15 citations) ELISA MAX Deluxe Set kits (14 citations) ELISA MAX™ Deluxe Set human IFN-γ kit (8 citations) ELISA MAXTM Deluxe kit (6 citations) ELISA MAX Standard Set kits (5 citations) Mouse-IFNγ ELISA MAX Kit (4 citations) LEGEND MAXTM ELISA kit (3 citations) Mouse IL-6 ELISA Max™ Set Deluxe Kits (3 citations) ELISA MAX deluxe set mouse kits (3 citations) LEGEND MAX™ Human IL-2 ELISA Kit (3 citations)

48 protocols using elisa max kit

1

Quantifying Cerebral IL-6 and Microglia-Neuron TNF-α

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Whole brain lysates were equally loaded onto a 96-well plate and cerebral IL-6 concentration was determined using ELISA Max Biolegend kit per manufacturer’s instructions. Media from microglia-neuron trans-well experiments were equally loaded onto a 96-well plate and culture TNF-α concentration was determined using ELISA Max Biolegend kit per manufacturer’s instructions (Biolegend, San Diego, CA, USA).
LeBlanc RH I.I.I., Chen R., Selim M.H, & Hanafy K.A. (2016). Heme oxygenase-1-mediated neuroprotection in subarachnoid hemorrhage via intracerebroventricular deferoxamine. Journal of Neuroinflammation, 13(1), 244.
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2

Cytokine Secretion Assay Protocol

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Human IFN-γ and TNF-α cytokine secretion was determined 24 hours after cell activation (with anti-CD3/anti-CD28 Abs), unless otherwise stated. ELISA MAX kits (BioLegend) were used according to manufacturer’s instructions. For mouse cytokine secretion assays, supernatants were harvested 24 hours after stimulation (with anti-CD3/anti-CD28 Abs, or IYSTVASSL) or 21–24 hours after restimulation with SIINFEKL. ELISA MAX kit (BioLegend) was used for mouse TNF-α analysis, according to manufacturer’s instructions. The mouse IFN-γ ELISA used coating antibody (BD Biosciences Rat anti-Mouse IFN-γ, Clone R4-6A2, 551216; used at 3 µg/mL), standards (eBioscience rmIFN-γ, 14-8311-63; top standard at 8 ng/mL), detection antibody (BioLegend biotin-Rat anti-Mouse IFN-γ, Clone XMG1.2, 505804; used at 0.5 µg/mL), and streptavidin (BioLegend, 405210; used 1:1000) following BioLegend’s ELISA MAX mouse TNF-α protocol.
Estrada L.D., Ağaç D, & Farrar J.D. (2016). Sympathetic neural signaling via the β2-adrenergic receptor suppresses T-cell receptor-mediated human and mouse CD8+ T-cell effector function. European journal of immunology, 46(8), 1948-1958.
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3

Mouse Blood Collection and Cytokine Analysis

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After sacrificing the mice, as much blood as possible was collected from the heart. Blood was allowed to clot for 30 mins at room temperature after which it was centrifuged at 1000 g for 10 mins at 4°C. Supernatant was collected and stored in −80°C until further use. ELISA for mouse IL-2 and IFN-γ was then performed using ELISA Max kit (Biolegend) according to manufacturer’s instructions.
Gupta S.S., Sharp R., Hofferek C., Kuai L., Dorn GW I.I., Wang J, & Chen M. (2019). NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells. Cell reports, 29(7), 1862-1877.e7.
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4

Evaluating CTCL Inhibition by NK Cells

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Target CTCL cells of 1.5 × 105 were incubated with biotinylated mAb14 at 10 µg/mL, biotin Isotype mouse IgG1ĸ (BioLegends, San Diego, CA, USA), or no antibody for 1 h at room temperature. A total of 5 × 104 cells of primary NK or NK92-MI (effector) cells were then co-cultured with these target cells (effector/target ratio, 1:3) in 96-well U-bottom plates for 20 h in an incubator. The concentration of secreted IFNγ was analyzed in cell supernatant using an ELISA MAX kit (BioLegend) and read at 650 nm with a microplate reader (BioTek, Winooski, VT, USA). Since the IFN-γ level in the co-culture of pNK cells with the CTCL cell line is attributed by to the two kind of cells, with no option to discriminate between them, we compared it to the sum of IFN-γ secretion according to each cell alone.
Knaneh J., Hodak E., Fedida-Metula S., Edri A., Eren R., Yoffe Y., Amitay-Laish I., Prag Naveh H., Lubin I., Porgador A, & Moyal L. (2023). mAb14, a Monoclonal Antibody against Cell Surface PCNA: A Potential Tool for Sezary Syndrome Diagnosis and Targeted Immunotherapy. Cancers, 15(17), 4421.
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5

Quantitative HCV RNA and Chemokine Analysis

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Serum HCV RNA was quantitated using the Cobas TaqMan real-time PCR (Roche Molecular Diagnostics, Branchburg NJ) with a lower limit of detection of 10 IU/ml and a lower limit of quantification of 25 IU/ml. Serum CXCL10 and CXCL11 were quantitated using the ELISA MAX kit (Biolegend, San Diego, CA) and the Quantikine ELISA kit (R&D Systems, Minneapolis, MN), respectively.
Serti E., Chepa-Lotrea X., Kim Y.J., Keane M., Fryzek N., Liang T.J., Ghany M, & Rehermann B. (2015). Successful Interferon-Free Therapy of Chronic Hepatitis C Virus Infection Normalizes Natural Killer Cell Function. Gastroenterology, 149(1), 190-200.e2.
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6

Mouse Blood Collection and Cytokine Analysis

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After sacrificing the mice, as much blood as possible was collected from the heart. Blood was allowed to clot for 30 mins at room temperature after which it was centrifuged at 1000 g for 10 mins at 4°C. Supernatant was collected and stored in −80°C until further use. ELISA for mouse IL-2 and IFN-γ was then performed using ELISA Max kit (Biolegend) according to manufacturer’s instructions.
Gupta S.S., Sharp R., Hofferek C., Kuai L., Dorn GW I.I., Wang J, & Chen M. (2019). NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells. Cell reports, 29(7), 1862-1877.e7.
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7

Quantification of Cytokine Levels

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IL-10, IL-17a, IL-21 and TGFβ1 were measured using the ELISA MAX™ kit (BioLegend), Ready-SET-Go! ELISA kits (eBioscience) and mouse TGFβ-1 DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), respectively, according to the protocols provided by the manufacturers and the results were analyzed on a microplate spectrophotometer (Perkin Elmer).
Pearson J.A., Peng J., Huang J., Yu X., Tai N., Hu Y., Sha S., Flavell R.A., Zhao H., Wong F.S, & Wen L. (2023). NLRP6 deficiency expands a novel CD103+ B cell population that confers immune tolerance in NOD mice. Frontiers in Immunology, 14, 1147925.
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8

T Cell-Tumor Co-culture Cytokine Assay

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5 × 104 T cells and 5 × 104 tumor
cells were co-cultured in 250 μl media without IL-2 in round bottom 96
well plates for 24 hours. Culture supernatants were collected and analyzed by
ELISA. IL-2 and IFNγ were detected with the ELISA MAX kit (Biolegend) and
TNF-alpha was detected with the Quantikine kit (R&D Systems). Bead-based
multiplex cytokine detection assays were performed at the Human Immune
Monitoring Center (Stanford University) using the Luminex platform.
Non-transduced T cells, which were CD3/28 activated but not retrovirally
transduced or gene-edited, were included as a negative control.
Freitas K.A., Belk J.A., Sotillo E., Quinn P.J., Ramello M.C., Malipatlolla M., Daniel B., Sandor K., Klysz D., Bjelajac J., Xu P., Burdsall K.A., Tieu V., Duong V.T., Donovan M.G., Weber E.W., Chang H.Y., Majzner R.G., Espinosa J.M., Satpathy A.T, & Mackall C.L. (2022). Enhanced T Cell Effector Activity by Targeting Mediator Kinase Module. Science (New York, N.Y.), 378(6620), eabn5647.
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9

Murine γδTCR-transduced E6.1 cells

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E6.1 cells were transduced with murine γδTCRs and 5x104 cells were co-cultured with 2x105 MODE-K expressing EV or l1l6 at 37°C for 24 hours. As control, E6.1 cells were stimulated with 1 μg/ml of α–CD3ε (OKT3) or IgG2a soluble antibodies. After 24h, supernatants were collected and secreted IL-2 was measured using the Elisa Max Kit (Biolegend) following the manufacturer’s instructions. 450nm absorbance was measured on an Infinite 200 PRO (Tecan) plate reader.
Melandri D., Zlatareva I., Chaleil R.A., Dart R.J., Chancellor A., Nussbaumer O., Polyakova O., Roberts N.A., Wesch D., Kabelitz D., Irving P.M., John S., Mansour S., Bates P.A., Vantourout P, & Hayday A.C. (2018). The γδ T cell receptor combines innate with adaptive immunity by utilizing spatially distinct regions for agonist-selection and antigen responsiveness. Nature immunology, 19(12), 1352-1365.
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10

Quantification of HCV RNA and CXCL10

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Serum HCV RNA levels were quantitated using the Cobas TaqMan real-time PCR assay (Roche Diagnostics, Palo Alto, CA), with a lower limit of detection of 10 IU/ml and lower limit of quantification of 25–43 IU/ml (Fig. 1). Serum CXCL10 was quantitated using the ELISA MAX kit (Biolegend, San Diego, CA) following the manufacturer’s protocol.
Serti E., Park H., Keane M., O’Keefe A.C., Rivera E., Liang T.J., Ghany M, & Rehermann B. (2016). Rapid Decrease in Hepatitis C Viremia by Direct Acting Antivirals Improves the Natural Killer Cell Response to IFNα. Gut, 66(4), 724-735.
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