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Deltagene

Manufactured by Standard BioTools
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The DeltaGene is a laboratory instrument designed for targeted gene expression analysis. It utilizes advanced digital PCR technology to precisely quantify and profile gene expression levels in biological samples. The DeltaGene provides accurate and reliable data to support research in areas such as molecular biology, genomics, and translational medicine.

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Lab products found in correlation

Preamp master mix, by Thermo Fisher Scientific (2 mentions) High capacity rt kit, by Thermo Fisher Scientific (2 mentions) Exonuclease 1, by New England Biolabs (2 mentions) Biomark hd, by Standard BioTools (2 mentions) Real time pcr analysis software, by Standard BioTools (2 mentions)

Close spelling variants of this product

DELTAgene assays (12 citations) DELTAgene Assay primers (3 citations)

2 protocols using deltagene

1

Quantifying Interferon-Stimulated Genes in PBMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from human PBMCs were converted to cDNA (High Capacity RT kit, Life Technologies) and preamplified by limited PCR (PreAmp Master Mix, Life Technologies) with a pool of 96 primers (DeltaGene, Fluidigm). We looked at 38 ISGs in this pool of 96 primers53 (link)-56 (link). Preamplified cDNAs were treated with ExonucleaseI (New England Biolabs) and diluted to remove unused primers and dNTPs and loaded onto a 96.96 Dynamic Array IFC for real-time PCR analysis on a BioMarkHD (Fluidigm). Analysis was performed, using Fluidigm’s Real Time PCR Analysis Software to determine Ct values, using linear (derivative) baseline correction and auto-detected, assay-specific threshold determination. Ct values were standardized to 3 housekeeping genes (DOCK2, EEF1A1, and FAM105B) that showed high correlation across samples to correct for sample input differences57 . Four samples from same donor and same draw were run on both Fluidigm assays. The mean of the Ct values were used for analysis. Human sample size was based on availability of IFIH1NR/NR and IFIH1R/R subjects.
Gorman J.A., Hundhausen C., Errett J.S., Stone A.E., Allenspach E.J., Ge Y., Arkatkar T., Clough C., Dai X., Khim S., Pestal K., Liggitt D., Cerosaletti K., Stetson D.B., James R.G., Oukka M., Concannon P., Gale M J.r., Buckner J.H, & Rawlings D.J. (2017). The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity. Nature immunology, 18(7), 744-752.
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2

Quantifying Interferon-Stimulated Genes in PBMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from human PBMCs were converted to cDNA (High Capacity RT kit, Life Technologies) and preamplified by limited PCR (PreAmp Master Mix, Life Technologies) with a pool of 96 primers (DeltaGene, Fluidigm). We looked at 38 ISGs in this pool of 96 primers53 (link)-56 (link). Preamplified cDNAs were treated with ExonucleaseI (New England Biolabs) and diluted to remove unused primers and dNTPs and loaded onto a 96.96 Dynamic Array IFC for real-time PCR analysis on a BioMarkHD (Fluidigm). Analysis was performed, using Fluidigm’s Real Time PCR Analysis Software to determine Ct values, using linear (derivative) baseline correction and auto-detected, assay-specific threshold determination. Ct values were standardized to 3 housekeeping genes (DOCK2, EEF1A1, and FAM105B) that showed high correlation across samples to correct for sample input differences57 . Four samples from same donor and same draw were run on both Fluidigm assays. The mean of the Ct values were used for analysis. Human sample size was based on availability of IFIH1NR/NR and IFIH1R/R subjects.
Gorman J.A., Hundhausen C., Errett J.S., Stone A.E., Allenspach E.J., Ge Y., Arkatkar T., Clough C., Dai X., Khim S., Pestal K., Liggitt D., Cerosaletti K., Stetson D.B., James R.G., Oukka M., Concannon P., Gale M J.r., Buckner J.H, & Rawlings D.J. (2017). The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity. Nature immunology, 18(7), 744-752.
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