Live cell imaging system

Manufactured by Zeiss

Sourced in Germany

The Live Cell Imaging System is a laboratory equipment designed for the observation and analysis of living cells in real-time. It enables users to capture high-quality images and video recordings of cellular processes and dynamics.

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Lab products found in correlation

Axiocam 506 color microscope, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Ab154193, by Abcam (1 mentions) Ab137485, by Abcam (1 mentions) F4680, by Merck Group (1 mentions) Alexa fluor 488 or 568 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Differential interference contrast microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Axio Observer Z1 Live-Cell imaging system Inverted fluorescence live cell imaging system Spinning disk live-cell imaging system

8 protocols using live cell imaging system

Wound-Healing Assay for Cancer Cell Migration

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A wound-healing assay was performed to examine the capability of cancer cell migration, as previously described^{40 (link)}. Briefly, GC cells were grown in 24-well plates with RPMI-1640 medium supplemented with 10% FBS up to 90% confluence. A single scratch wound was generated with a 10 μL pipette tip. After removing the suspension cells by washing with PBS, fresh RPMI-1640 medium without FBS was added. With a Live Cell Imaging System (ZEISS, Germany), moving and growing of cells across the scratched lines were monitored every hour for 24 h. The migratory ability of the cells was presented as the gap distance recovered compared with the original gap.

Hou X.L., Ji C.D., Tang J., Wang Y.X., Xiang D.F., Li H.Q., Liu W.W., Wang J.X., Yan H.Z., Wang Y., Zhang P., Cui Y.H., Wang J.M., Bian X.W, & Liu W. (2017). FPR2 promotes invasion and metastasis of gastric cancer cells and predicts the prognosis of patients. Scientific Reports, 7, 3153.

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Cell Migration Assay Protocol

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GC cells were grown in 24-well plates with RPMI-1640 medium supplemented with 10% FBS until 90% confluence. Wounds were created by scratching the cell surface with a 10 μL pipette tip. After washing with PBS, fresh RPMI-1640 medium without FBS was added. Cells moving and growing across the scratched lines were monitored every hour for 24 h using a Live Cell Imaging System (ZEISS, Germany). The migratory ability of the cells was quantified as the gap distance recovered compared with the original gap.

Liu J.J., Liu J.Y., Chen J., Wu Y.X., Yan P., Ji C.D., Wang Y.X., Xiang D.F., Zhang X., Zhang P., Cui Y.H., Wang J.M., Bian X.W, & Qian F. (2016). Scinderin promotes the invasion and metastasis of gastric cancer cells and predicts the outcome of patients. Cancer letters, 376(1), 110-117.

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Mitotic Morphology Characterization

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Cells were stained with NucSpot^Ⓡ Live Cell Nuclear Stains (#40082, BIOTIUM, Fremont, CA). Washings were with phosphate buffered saline (PBS) before plating in RPMI 1640 media supplemented with 10% FBS with antimycotics and antibiotics. Cells were maintained in the microscope chamber at 37°C and 5% CO₂. Stained cells were scored for mitotic figures that were bipolar, or those having ring-like chromosomal structures or multipolarity division using a ZEISS Axiocam 506 color microscope and the Live-Cell Imaging system (Carl Zeiss Microscopy LLC, White Plains, NY).

Chen Z., Liu X., Kawakami M., Liu X., Baker A., Bhatawadekar A., Tyutyunyk-Massey L., Narayan K, & Dmitrovsky E. (2023). CDK2 inhibition disorders centrosome stoichiometry and alters cellular outcomes in aneuploid cancer cells. Cancer Biology & Therapy, 24(1), 2279241.

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Live Imaging of Glioma Cells

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Glioma cells were transduced with the H2B‐GFP fusion protein using the lentivirus vector pCDH‐CMV‐MCS‐EF1‐Puro, and positive cells were sorted using flow cytometry. Then, the cells were plated in six‐well plates and incubated overnight. The plates were incubated in a live cell imaging system (Zeiss) with normal culture conditions. Images were captured every 5 min for 48 h and were processed and analyzed by AxioVision software (Zeiss).

Zhu Z., Wang J., Tan J., Yao Y., He Z., Xie X., Yan Z., Fu W., Liu Q., Wang Y., Luo T, & Bian X. (2021). Calcyphosine promotes the proliferation of glioma cells and serves as a potential therapeutic target. The Journal of Pathology, 255(4), 374-386.

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Hydrogel Extraction Effect on HSF Cell Morphology

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HSF cells were cultured with hydrogel extraction solutions (pH 3, 7.4 and 8.5) in a 24-well plate (10⁴ cells/well) for 24 h. After that, the medium was removed and washed with 1X PBS buffer to remove unattached cells. After the washing procedure, 2 mL 4 % paraformaldehyde in PBS was added into each well for 15 min to fixed cells, and the cells were washed with 1X PBS buffer three times. Then 1 mL 1 % BSA was added into each well to incubate at 37 °C for 30 min. BSA was removed and washed by 1X PBS buffer. Finally, diluted rhodamine phalloidin (100 μL, 1 μg/mL) was added into each well for 1 h and DAPI (100 μL, 0.5 μg/mL) was added into each well for 3 min, respectively. The cell morphology was observed by a Live Cell Imaging System (Zeiss Cell Observer).

Liu B., Li J., Zhang Z., Roland J.D, & Lee B.P. (2022). pH Responsive Antibacterial Hydrogel Utilizing Catechol–Boronate Complexation Chemistry. Chemical engineering journal (Lausanne, Switzerland : 1996), 441, 135808.

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Coculture of CNE-2 and GFP-Akata Cells

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CNE-2 or NEPC-Bmi1 cells pre-stained with CellTracker Red were planted in 35-mm confocal dish at 1 × 10⁴ cells per well in RPMI 1640 medium containing 10% FBS. After 12 h, the culture medium was removed and GFP-Akata or AK31 cells at 1 × 10⁵ cells in 1 ml culture medium were added into the dish. Cells were incubated at 37 °C with 5% CO₂. After 4 h co-culture, non-adherent GFP-Akata cells were removed, followed by live cell Imaging System (Carl Zeiss Inc) observation for 12-72 h.

Ni C., Chen Y., Zeng M., Pei R., Du Y., Tang L., Wang M., Hu Y., Zhu H., He M., Wei X., Wang S., Ning X., Wang M., Wang J., Ma L., Chen X., Sun Q., Tang H., Wang Y, & Wang X. (2015). In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures. Cell Research, 25(7), 785-800.

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Immunofluorescence Localization of CXCR7 in Tissues

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Immunofluorescence staining was used to determine the location of CXCR7. Briefly, the tissue sections were fixed with 4% paraformaldehyde for 30 min. After treatment with 0.6% Triton X‐100 for 1 hr, the sections were blocked with donkey serum. Then, the sections were incubated overnight at 4°C with primary antibodies including anti‐CXCR7 (1:200, Abcam, ab137485) and anti‐vWF (1:200, Abcam, ab154193), followed by incubating with Alexa Fluor 488‐ or 568‐conjugated secondary antibody was added (1:500, Life Technologies). Samples were then co‐stained with DAPI (Sigma‐Aldrich) for 15 min at room temperature. Cover glasses were mounted with aqueous mounting medium (Sigma‐Aldrich, F4680). The images were obtained using a Zeiss Live Cell Imaging System.

Qiu L., Zhang M., Zhang S., Tang Y., Zhang Y., Li C., Wang Y., Jiang L, & Zheng J.C. (2020). Activation of CXCR7 promotes endothelial repair and reduces the carotid atherosclerotic lesions through inhibition of pyroptosis signaling pathways. Aging Cell, 19(9), e13205.

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Corneal Epithelial Cell Apoptosis

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The cells were irradiated with 144-mJ/cm 2 UVB for 2 h and then cultured in the Live Cell Imaging System (Zeiss) at 37°C with 5% CO 2 for 0, 6, and 12 h such that the experimental groups were called "I (2h) + C (0 h)," "I (2h) + C (6 h)," and "I (2h) + C (12 h)." The morphological changes in the corneal epithelial cells after irradiation at 0, 6, and 12 h were recorded using a differential interference contrast microscope (Zeiss). Then, 100 cells were chosen randomly, and the numbers of the different kinds of apoptotic cells were counted using the ImageJ software (National Institutes of Health, MD, USA).

Du S., Li J., Chen L., Niu Z, & Gao L. (2021). Dynamic changes in and time sequence of ultraviolet B-induced apoptosis in rat corneal epithelial cells. Arquivos brasileiros de oftalmologia, 85(4).

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