Human liver cancer cells (HCC-LM3, Huh7, Hep-G2, Hep-G2.215, SK-Hep1 and 7721) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. The human liver cell line HL-7702 (L02) was purchased from Wuhan Hengyisai Biotechnology Co., Ltd. Short tandem repeat (STR) profiling was used to identify all cells. All cells were cultured in an incubator at 37°C and 5% carbon dioxide using DMEM with 10% FBS.
Hepg2
Manufactured by Shanghai Cell Bank
Sourced in China
HepG2 is a well-characterized human liver cancer cell line. It is commonly used in various in vitro studies, including hepatocyte-related research and toxicology assessments.
Automatically generated - may contain errors
Lab products found in correlation
Hep3b, by Shanghai Cell Bank (31 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (29 mentions)
Smmc 7721, by Shanghai Cell Bank (26 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (18 mentions)
Hcclm3, by Shanghai Cell Bank (17 mentions)
Sk hep 1, by Shanghai Cell Bank (16 mentions)
Bel 7402, by Shanghai Cell Bank (15 mentions)
Mhcc 97h, by Shanghai Cell Bank (12 mentions)
Plc prf 5, by Shanghai Cell Bank (10 mentions)
Hepg2, by Thermo Fisher Scientific (10 mentions)
Hl 7702, by Shanghai Cell Bank (8 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (8 mentions)
Mhcc97l, by Shanghai Cell Bank (7 mentions)
Bel 7404, by Shanghai Cell Bank (7 mentions)
Qgy7701, by Shanghai Cell Bank (5 mentions)
Smmc 7721, by Thermo Fisher Scientific (4 mentions)
Hep3b, by Thermo Fisher Scientific (4 mentions)
Bel 7402, by Thermo Fisher Scientific (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (4 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
88 protocols using hepg2
1
Characterization of Liver Cancer Cell Lines
2
Hepatocellular Carcinoma Cell Line Maintenance
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HHCC, H-97, HepG2, HuH-7, Li-7, SMMC-7721, HepG2.2.15 and HL-7702 cell lines were purchased from the Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). Cells were maintained in Dulbecco’s Modified Eagle medium (DMEM) (Hyclone, UT, USA) with 10% fetal bovine serum (FBS, Beyotime, Nantong, China), 100 μg/ml streptomycin and 100 IU/ml penicillin (Invitrogen, USA) at 37°C, 5% CO2. For transfections, cells at the confluence of 50–80% were infected with 1 × 106 recombinant lentivirus-transducing units and 6 μg/mL Polybrene (Sigma, Shanghai, China). Stably transfected cells were selected via treatment with 2μg/mL puromycin for 2 weeks. Stably transfected cells were picked via flow cytometry for subsequent assays. Plasmid, lentivirus, miRNA inhibitor and miRNA mimics used in this study were purchased from GenePharma Co., Ltd. (Shanghai, China), pHBV1.3 copy was purchased from Miaolingbio (Wuhan, China). Lipofectamine 3000 (Invitrogen, CA, USA) was utilized for transfection.
3
Multifunctional Nanoparticle-Based Cancer Therapy
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All reagents and solvents were purchased from commercial suppliers and used without further purification. HAuCl4 (98%) and 3-mercaptopropionic acid (MPA; 98%) were purchased from Sigma-Aldrich, while polyethylene glycol (PEG 2000) (N/A), hyaluronic acid (HA; 403, 97%), adipic dihydrazide (ADH; 98%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; 99%), and N-hydroxy succinimide (NHS; 98%) were purchased from J&K Scientific, while doxorubicin (Dox; 97%) was from Aladdin. Human normal hepatocytes (QSG-7701), human hepatocellular liver carcinoma cells (HepG2), cervical cancer cells (HeLa), and colon cancer cells (HCT-116) were purchased from the Chinese Academy of Sciences Shanghai Cell Bank.
4
Culturing Huh7 and HepG2 Liver Cells
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The human liver cell lines Huh7 and HepG2 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. They were resuspended in DMEM high glucose (containing 10% FBS and double antibodies). The cells were cultured at 37 °C and 5% CO2 until the confluence was approximately 90%.
5
Cultivation of Hepatocellular Carcinoma Cell Lines
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Human hepatocellular carcinoma cell lines including HepG2, SMMC-7721, Huh7 and Hep3B, and human liver cells HL7702 were purchased from Cell Bank of Shanghai Institute of Biochemistry & Cell Biology, Chinese Academy of Sciences. Cells were cultured in RPMI-1640 medium (Invitrogen, USA) and supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin at 37°C in 5% CO2.
6
Cell Culture of Hepatic Cell Lines
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The HepG2, 7721, Huh7 and LO2 cell lines were purchased from the Shanghai Cell Bank (Chinese Academy of Sciences, Shanghai, China). The basic cell culture medium was high-glucose DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS). The cells were cultured in an incubator with 5% CO2 at 37℃.
7
Fluorescent Mice and Cell Line Protocols
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HepG2 was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, 4T1 was purchased from the University of Pittsburgh Medical Center, and DEX was purchased from Anhui Fengyuan Pharmaceutical Co., Ltd. Fluorescent nude mice and BALB/c mice were provided by the Experimental Animal Center of Soochow University. Individual ventilated caging (IVC) mice were independently supplied with air. The feeding system was purchased from Soochow Suhang Equipment Co., Ltd.; DMEM medium and RPMI-1640 medium were purchased from HyClone, Logan, UT, USA; the fluorescence microscope was purchased from Zeiss, Oberkochen, Germany; fluorescent lights and lenses were purchased from Nightsea, Lexington, MA, USA; the frozen slicer was purchased from Leica, Buffalo Grove, IL, USA; and the flow cytometer was purchased from Beckman CytoFLEX, Krefeld, Germany.
8
Culturing Human Hepatocellular Carcinoma Cells
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The normal hepatocyte LO2 cells, as well as the commercially available HCC cell lines Huh-7, HepG2, HCC-LM3, SMMC-7721 and MHCC-97L, were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Home-made patient-derived HCC cell lines PDC-26#, PDC-14#, PDC-12#, PDC-9#, PDC-23#, PDC-10# and PDC-11# were established as described above. All of these cells were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% (v/v) FBS and 1% penicillin-streptomycin at 37 °C in a humidified incubator under 5% CO2 condition.
9
Establishment and Maintenance of Cancer Cell Lines
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The human breast cancer cell lines MDA-MB-231, MCF-7 and T-47D, human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, human nasopharyngeal carcinoma cell lines HNE1 and CNE-2Z, human gastric cancer cell line SGC7901, human endometrial cancer cell line ISK, and human non-small cell lung cancer cell line A549 were bought from Shanghai Cell Bank (Shanghai, China). The human colon carcinoma SW480 was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were obtained, frozen and cultured in our laboratory. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) or Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco), supplemented with 10 % fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were grown in an atmosphere containing 5%CO2 at 37 °C.
10
Culturing and Transfecting HCC Cell Lines
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The HCC cell lines, HepG2, Hep3B, Huh-7 and SMMC-7221, were purchased from Shanghai Cell Bank (Shanghai, China) and were cultured in media according to the manufacturer’s instructions. HepG2 cells stably expressing hSulf-1 were selected with 600 μg/ml G418 (Invitrogen Life Technologies) and the transfection results were detected by western blotting. Cells were maintained at 37°C in an atmosphere of humidified air with 5% CO2.
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