Log In Sign up
The largest database of trusted experimental protocols

Nucleocounter nc 3000 cytometer

Manufactured by ChemoMetec
Visit Supplier

The NucleoCounter NC-3000 is a fully automated cell counter and viability analyzer. It utilizes fluorescence microscopy to quickly and accurately determine the total cell count and viability of a sample.

Automatically generated - may contain errors

Lab products found in correlation

Annexin 5 binding buffer, by Thermo Fisher Scientific (1 mentions) Annexin 5 cf488a conjugate, by Biotium (1 mentions) Hoechst 33342, by ChemoMetec (1 mentions) Propidium iodide, by ChemoMetec (1 mentions)

Spelling variants of this product

NucleoCounter NC-3000 (229 citations) NucleoCounter (85 citations) NC-3000 (23 citations) NucleoCounter 3000 (12 citations) NucleoCounter® NC-3000™ image cytometer (6 citations) Advanced Image Cytometer NucleoCounter NC-3000 (4 citations)

2 protocols using nucleocounter nc 3000 cytometer

1

Annexin V Apoptosis Assay in Organoids

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Annexin V assay was performed64 (link),65 (link) by first dissociating cortical organoids and monolayer neurons, which were subsequently resuspended in Annexin V binding buffer (Invitrogen) with Annexin V-CF488A conjugate (Biotium, Inc., Hayward, CA, USA) and Hoechst 33342 (Chemometec) and incubated for 15 min at 37°C. After a PBS wash, the cells were resuspended in Annexin V binding buffer (Invitrogen) supplemented by 10 μg/mL propidium iodide (Chemometec). Cells were loaded into NC-Slide A2 chambers and assessed with a Chemometec NucleoCounter NC-3000 cytometer using the preoptimized Annexin V assay.
Adams J.W., Vinokur A., de Souza J.S., Austria C., Guerra B.S., Herai R.H., Wahlin K.J, & Muotri A.R. (2024). Loss of GTF2I promotes neuronal apoptosis and synaptic reduction in human cellular models of neurodevelopment. Cell reports, 43(3), 113867.
+ Open protocol
+ Expand
2

Enumeration of HSPC Colonies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For each assay, 500–1000 CD34+ HSPCs were plated in 1 mL of methylcellulose media (Stem Cell Technologies, catalog 4435) in triplicate in 35-mm tissue culture dishes. Colonies were enumerated at 14 days after plating. Cells were pooled, washed with PBS, and counted using a NucleoCounter NC-3000 cytometer (ChemoMetec) to determine the cell count per colony.
Bhoopalan S.V., Yen J.S., Mayuranathan T., Mayberry K.D., Yao Y., Lillo Osuna M.A., Jang Y., Liyanage J.S., Blanc L., Ellis S.R., Wlodarski M.W, & Weiss M.J. (2023). An RPS19-edited model for Diamond-Blackfan anemia reveals TP53-dependent impairment of hematopoietic stem cell activity. JCI Insight, 8(1), e161810.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!