Ae300s

Manufactured by Minato Medical Science

Sourced in Japan

The AE300S is a laboratory equipment product. It is designed for analytical purposes. The core function of the AE300S is to perform measurements and analyses.

Automatically generated - may contain errors

Lab products found in correlation

Accurex plus, by Polar Electro (6 mentions) Ml 9000, by Fukuda Denshi (3 mentions) Fb 300, by Fukuda Denshi (3 mentions) Ml 6500, by Fukuda Denshi (2 mentions) Acculex plus, by Polar Electro (2 mentions) Mat 2700, by Fukuda Denshi (1 mentions) Corival, by Lode Ergometry (1 mentions) Smart pulse, by Fukuda Denshi (1 mentions) Ae 310, by Minato Medical Science (1 mentions) Strengthergo 8, by Mitsubishi (1 mentions)

55 protocols using ae300s

Respiratory Gas Analysis during Fasting and Exercise

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Resting respiratory gas samples were collected on days 1 and 2 following overnight fasting (8:10–8:40). Participants sat on comfortable chair followed by sufficient rest (at least 20 min), and respiratory gas samples were measured during sitting for 10 min using an automatic gas analyzer (AE 300S; Minato Medical Science Co., Ltd., Osaka, Japan).
The values were averaged every 30 s. The data during the last 3 min were utilized for further analyses. Also, respiratory gas samples during exercise were evaluated for 27–30 min during 60 min of exercise on day 2 using the same analyzer (AE 300S; Minato Medical Science Co., Ltd., Osaka, Japan). The values were averaged every 30 s. The data were utilized for the last 3 min for further analyses. From the

\dot{V} O_{2}

and

\dot{V} {CO}_{2}

, substrate (carbohydrate and fat) oxidation during exercise was estimated using Frayn’s equation [21 (link)].

Kojima C., Ishibashi A., Ebi K, & Goto K. (2022). Exogenous glucose oxidation during endurance exercise under low energy availability. PLoS ONE, 17(10), e0276002.

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Cardiopulmonary Exercise Testing Equipment

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The breath-by-breath gas analyzers used in this study were the CPEX-1 system manufactured by Inter Reha Co. Ltd. (Tokyo, Japan) and the AE-300S manufactured by Minato Medical Science Co. Ltd. (Osaka, Japan). All analyzers were carefully calibrated before the start of this project. Cycle ergometers used in this study were the SE-8, a servomotor-controlled model by Mitsubishi Electric Engineering Co. Ltd. (Nagoya, Japan) and the Corival 400, an electromagnetically braked cycle ergometer (Lode BV, Groningen, The Netherlands). The stress systems used were the ML-4500, ML-6500, and ML-9000 manufactured by Fukuda Denshi Co. Ltd. (Tokyo, Japan). Each of these models can monitor 12-lead ECGs simultaneously while also controlling a cycle ergometer and automatic-cuff blood pressure manometer (FB-300; Fukuda Denshi Co. Ltd. or STBP-780; Nippon Colin Co. Ltd., Komaki, Japan, or Tango; SunTech Medical, Inc., Morrisville, NC, USA).

Ashikaga K., Itoh H., Maeda T., Itoh H., Ichikawa Y., Tanaka S., Ajisaka R., Koike A., Makita S., Omiya K., Kato Y., Adachi H., Nagayama M., Tajima A., Harada N, & Akashi Y.J. (2021). Ventilatory efficiency during ramp exercise in relation to age and sex in a healthy Japanese population. Journal of cardiology, 77(1).

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Symptom-Limited Cardiopulmonary Exercise Testing

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Symptom-limited CPET was performed on an electromagnetically braked upright cycle ergometer (Corival, Load, Holland) with a metabolic gas analyzer (AE-300S; Minato Medical Science, Osaka, Japan). After 4 min of rest on the cycle ergometer, exercise commenced at 20 watt for a 4 min-warm up; then, the work rate was increased by 1 watt every 6 s. During CPET, blood pressure was measured by an automatic, indirect cuff manometer (FB-300; Fukuda Denshi, Tokyo, Japan) every min. HR and electrocardiography (ECG) were monitored using an exercise electrocardiogram (ML-9000; Fukuda Denshi, Tokyo, Japan). The criteria for discontinuation of CPET were (i) if pedal rotations were delayed; (ii) if the patient reached maximum symptom-limited performance determined by a Borg score of ≥17; (iii) when 85% of age-predicated maximal HR was achieved; or (iv) if there was evidence of ST-T changes in ECG or if any cardiac event, such as arrhythmia or chest pain, occurred. Expired gases were continuously measured in all subjects on a breath-by-breath basis. The anaerobic threshold (AT) was determined by gas-exchange criteria as the point of nonlinear increase in ventilation equivalents for oxygen.

Takano N., Amiya E., Oguri G., Nakayama A., Taya M., Nakajima T., Morita H, & Komuro I. (2019). Influence of atrial fibrillation on oxygen uptake and exercise tolerance in cardiovascular patients; close association with heart rate response. International Journal of Cardiology. Heart & Vasculature, 22, 84-91.

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Respiratory Gas Analysis during Exercise

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Respiratory gases were collected at 25–30 min and 55–60 min using an automatic gas analyzer (AE300S; Minato Medical Science, Tokyo, Japan) to evaluate

\dot{V}

O₂,

\dot{V}

CO₂

\dot{V}

E, and the respiratory exchange ratio (RER). Energy expenditure was calculated as 3.9 ×

\dot{V}

O₂ + 1.1 ×

\dot{V}

CO₂^{25 (link)}.

Hayashi N., Ishibashi A, & Goto K. (2018). Effects of diet before endurance exercise on hepcidin response in young untrained females. Journal of Exercise Nutrition & Biochemistry, 22(4), 55-61.

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Graded Pedaling Test for VO2max Determination

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The graded pedaling test to determine

\dot{V}

O_2max was conducted on a bicycle ergometer (Aerobike 75XLIII, Konami Sports Life Co., Ltd, Kanagawa, Japan). The initial load was 30 W with increments of 30 W every 2 min until 90 W was reached. Subsequently, the load was further increased by 20 W until exhaustion. Respiratory gases were collected throughout the exercise with an automatic gas analyzer (AE300S; Minato Medical Science, Tokyo, Japan). The sample during exercise was analyzed to determine the

\dot{V}

O_2max, and the workload at 65% of

\dot{V}

O_2max was calculated individually.

Hayashi N., Ishibashi A, & Goto K. (2018). Effects of diet before endurance exercise on hepcidin response in young untrained females. Journal of Exercise Nutrition & Biochemistry, 22(4), 55-61.

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Crossover Study of Thermoneutral Exposure Effects

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The study was a random crossover single-blind design (with or without TNP conditions on two separate days). In session 1, the participants performed either the TNP or control sessions. In session 2, they performed either the TNP or control sessions, all the participants completed both the experiments on two separate days within one week. To eliminate the influence of execution order, the allocation of the TNP or control to sessions 1 and 2 was randomized among participants. The participants in this study refrained from doing any strenuous physical activity, including general exercise, from the day prior to the start of the experiment, and they started fasting (no water restriction) four hours before starting the experiment. Height and body weight were measured before the exercise. For each participant, the resting oxygen consumption (VO₂) was measured in both sessions using an indirect calorimeter (AE-300s, Minato Medical Science Co., Ltd., Osaka, Japan) while sitting on a cycle ergometer and maintaining a resting position for 5 min with a face mask attached. After measuring the resting VO₂, each participant performed session 1 or 2. All the measurements were carried out in a laboratory, where the temperature and humidity of the internal atmosphere were adjusted to 20 °C and 50%, respectively.

Nakagata T., Fukao K., Kobayashi H., Katamoto S, & Naito H. (2019). The Effects of Transdermal Nicotine Patches on the Cardiorespiratory and Lactate Responses During Exercise from Light to Moderate Intensity: Implications for Exercise Prescription during Smoking Cessation. Medicina, 55(7), 348.

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Maximal Oxygen Uptake Testing Under Normoxia and Hypoxia

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The

\dot{V} o_{2max}

-test was conducted twice under normoxia (Fio₂: 20.9%) and hypoxia (Fio₂: 14.5%). The test began at 50 W, and the load was increased progressively by 30-W increments every 2 min until exhaustion [80 revolutions/min (rpm)]. During the test, expired gases were collected and analyzed using an automatic gas analyzer (AE300S; Minato Medical Science Co., Ltd., Tokyo, Japan). The respiratory data were averaged every 30 s. Heart rate (HR) was measured continuously during the test using a wireless HR monitor (Accurex Plus; Polar Electro Oy, Kempele, Finland). The order of the two repeated bouts of

\dot{V} o_{2max}

-tests under normoxia and hypoxia was randomized. These tests were performed a minimum of at least 3 days before the trials commenced.

Sumi D., Yamaguchi K, & Goto K. (2021). Impact of Three Consecutive Days of Endurance Training Under Hypoxia on Muscle Damage and Inflammatory Responses. Frontiers in Sports and Active Living, 3, 663095.

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Indirect Calorimetry for Resting Energy Expenditure

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REE was measured using an indirect calorimetry device (AE-300S, Minato Medical Science, Osaka, Japan). Indirect calorimetry was performed in a hospital room after an overnight (12 h) fast. Calibrations of flow rate and standard gas were performed before every measurement, and the room temperature was controlled at 23–25 °C. Patients rested for 30 min before undergoing measurements for 15 min between 7:00 and 8:00 a.m. A pump was used to suction through the hood at a constant rate. Using the Weir equation (Equation (1)) [19 (link)], the 24 h REE was calculated from the measured oxygen consumption (VO₂) and carbon dioxide production (VCO₂).

REE = (3.94 \times {\dot{V} O}_{2} + 1.11 \times {\dot{V} CO}_{2}) \times 1440

Kawase F., Masaki Y., Ozawa H., Imanaka M., Sugiyama A., Wada H., Goto R., Kobayashi S, & Tsukahara T. (2022). Resting Energy Expenditure in Older Inpatients: A Comparison of Prediction Equations and Measurements. Nutrients, 14(24), 5210.

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Symptom-limited Exercise Stress Test

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A symptom-limited exercise stress test, using the ramp method, was conducted using an expiration gas analyzer (AE300S, Minato Medical Science Co., Ltd., Osaka, Japan) and an ergometer cycle (AEROBIKE 75XL, Combi, Tokyo, Japan) with a 12-lead electrocardiogram. Exercise began with a four-minute warm-up at 10–20 W and 50 rpm, followed by the 10–20 W ramp method after a five-minute rest on the ergometer. Heart rate, oxygen uptake (VO₂), and carbon dioxide excretion volume (VCO₂) were measured at the point of rest, warm-up, anaerobic threshold (AT), and maximum oxygen uptake (peak VO₂) using the breath-by-breath method. The AT was determined using the V-slope method. Peak VO₂ and work rate were defined as the peak values during incremental exercise [20 ].

Takao N., Kurose S., Miyauchi T., Onishi K., Tamanoi A., Tsuyuguchi R., Fujii A., Yoshiuchi S., Takahashi K., Tsutsumi H, & Kimura Y. (2021). The relationship between changes in serum myostatin and adiponectin levels in patients with obesity undergoing a weight loss program. BMC Endocrine Disorders, 21, 147.

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Symptom-limited CPET for Exercise Physiology

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Symptom-limited CPET was performed on an electromagnetically braked upright cycle ergometer (Corival, Load, Holland) with a metabolic gas analyzer (AE-300S, Minato Medical Science, Osaka, Japan). After 4 min of rest on the cycle ergometer, exercise was commenced at 20 W for a 4-min warm-up, and then the work rate was increased by 1-W every 6 s. During CPET, blood pressure was measured by an automatic indirect cuff manometer (FB-300, Fukuda Denshi, Tokyo, Japan) every min. HR and electrocardiography (ECG) were monitored using an exercise electrocardiogram (ML-9000, Fukuda Denshi, Tokyo, Japan) [15] (link). The criteria for discontinuation of CPET were (i) if pedal rotations were delayed, (ii) if the patient reached maximum symptom-limited performance determined by a Borg score of ≥ 17, (iii) when 85% of age-predicted maximal HR (APMHR) was achieved, (iv) if there was evidence of ST-T changes in ECG, or if any cardiac event such as arrhythmia or chest pain occurred. Expired gases were measured continuously in all subjects on a breath-by-breath basis. The anaerobic threshold (AT) was determined by gas exchange criteria as the point of nonlinear increase in ventilation equivalents for oxygen. The mean VO₂ and HR at warm-up (Wu; 3–4 min after exercise commenced), at AT, at the respiratory compensation point (Rc), and at the exercise peak (Pk) were all measured and recorded.

Takano N., Takano H., Fukuda T., Kikuchi H., Oguri G., Fukumura K., Iwasawa K, & Nakajima T. (2014). Relationship between chronotropic incompetence and β-blockers based on changes in chronotropic response during cardiopulmonary exercise testing. International Journal of Cardiology. Heart & Vasculature, 6, 12-18.

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