Blood morphology was performed by using a hematology analyzer (Siemens Healthineers, Erlangen, Germany). Concentration of C-reactive protein (CRP) was estimated using the dry chemistry immunological method on a VITROS 250 analyzer (Ortho Clinical Diagnostics, Johnson and Johnson, Raritan, NJ, USA). Direct potentiometric measurement of Na+, K+ and Cl− in serum with liquid ion-exchange electrode was performed.
Vitros 250
Manufactured by Ortho Clinical Diagnostics
Sourced in United States
The Vitros 250 is an automated chemistry analyzer designed for in-vitro diagnostic testing. It is capable of performing a range of clinical chemistry and immunoassay tests on a variety of sample types, including serum, plasma, and urine.
Automatically generated - may contain errors
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Advia 120 hematology system, by Siemens (1 mentions)
Superscript 3, by Thermo Fisher Scientific (1 mentions)
Lightcycler 480, by Roche (1 mentions)
26 protocols using vitros 250
1
Comprehensive Blood Analysis Protocol
2
Hypocalcemia Induction via EGTA Infusion
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Hypocalcemia was induced by an intravenous infusion of EGTA (ethylene-bis(oxyethylenenitrilo)tetraacetic acid; Sigma, USA), 40 mM, at a rate of 3.0 ml/h for 30 minutes (from time 60 to 90 minutes) through a catheter inserted into the femoral vein. Plasma Ca2+ was measured by a Calcium Selective Electrode (ABL505, Radiometer, Copenhagen, Denmark). Plasma phosphate was analyzed by Vitros 250 (Ortho-Clinical Diagnostics, Raritan, NJ, USA).
3
Biochemical and Viral Marker Assays
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HBeAg and qualitative HBsAg were performed by an automated EIA method (Axsym, Abbott Diagnostics, Rungis, France). Biochemical parameters were determined by Vitros 250 instrument (Ortho Clinical Diagnostics, Issy-les-Moulineaux, France). Platelets were determined by Cell-Dyn 3700 (Abbott).
4
MERS-CoV Infection in AGMs
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Blood samples and throat swab specimens were collected at indicated days after aerosol exposure to MERS-CoV (Figure 1 ). Puritan 6-inch 25–800–1PD sterile swabs (https://www.puritanmedproducts.com ) were used for collection and were placed in 1-mL virus growth medium and frozen until further processing. Blood samples were collected from the femoral vein of AGMs anesthetized with 3 mg/kg intramuscular ketamine. Samples collected 7 days before exposure served as a reference baseline for each animal. Blood chemistry values were analyzed with VITROS 250 chemistry analyzers (Ortho Clinical Diagnostics, https://www.orthoclinicaldiagnostics.com ).
5
Aortic Calcium Content Analysis
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Plasma creatinine, urea, phosphate and total Ca were analyzed by Vitros 250 (Ortho-Clinical Diagnostics, Raritan, USA). Plasma Ca2+ was measured by ABL800 (Radiometer, Copenhagen, Denmark). Plasma intact FGF23 (iFGF23) was measured by a human FGF23 ELISA (Kainos Laboratories, Tokyo, Japan), with an intra-assay coefficient of variation of 2.5% and inter-assay coefficient of variation of 5% in our lab [49 (link)]. Plasma PTH was measured by a rat bioactive intact PTH ELISA assay (Immutopics, San Clemente, USA) with an intra-assay coefficient of variation of 4% and intra-assay variation of 9% in our lab [50 (link)]. Aorta Ca-content was determined by the o-cresolphthalein method and normalized to the dry weight. A small section of the proximal thoracic aorta was lyophilized for 24 hours to determine the dry weight. After lyophilisation the aorta section was decalcified in 1M HCl for 24 hours and the Ca-content of the supernatant was determined using a commercial assay (Sigma-Aldrich, St. Louis, USA).
6
Automated Hematology and Clinical Chemistry Analysis
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Complete blood counts (CBC) and blood differentials were determined using an Advia automated hematology instrument (Bayer Corporation, Tarrytown, NY, USA) (Whitnall et al. 2002 (link)). Complete clinical chemistry panels (19 analytes) were developed on the blood sera of mice using a J & J Vitros 250 instrument (Ortho-Clinical Diagnostics, Holliston, MA, USA) (Whitnall et al. 2002 (link)).
7
Vitros 250 Analyte Quantification
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Analyte concentrations were determined with a Vitros 250 (Ortho-Clinical Diagnostics, Markham, ON) with dry-slide matter.
8
Comprehensive Assessment of Vitamin D Status in Refugee Populations
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The following were recorded at the visits:
Clinical parameters: height, weight, clinical signs of rickets (bone deformities, widened epiphyses, craniotabes and rachitic rosary);
Biochemical parameters: serum 25(OH)D (DiaSorin Liaison, DiaSorin (PTY) LTD, NSW, Australia), calcium and alkaline phosphatase (ALP) (Abbott Architect, Abbott Diagnostics Division, NSW, Australia or on Vitros 250, Ortho Clinical Diagnostics Australia, VIC, Australia);
A sun exposure questionnaire was applied using the recall method (duration and timing of sun exposure four days before the visit, factors affecting sun exposure including weather, season, clothing, hat and sun screen). This information was transformed into a validated score system “sun exposure score (SES)” based on a method by Specker et al. [28 (link)]; with higher scores equivalent to greater sun exposure;
Nutrition was assessed using a three-day food diary. This information was analysed using software of the German Society for Nutrition (DGE-PC Professional Version 2.8.0.26, 2007) to assess average dietary calcium and vitamin D intake across the three major ethnic groups: Asian, Middle-Eastern and African;
Data on country of origin and country of refuge (last country of transit) were included.
9
Blood Morphology and CRP Evaluation
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Blood morphology was performed using hematology analyzer (Siemens Healthineers, Germany). C-reactive protein (CRP) was estimated using the dry chemistry immunological method on a VITROS 250 analyzer (Ortho Clinical Diagnostics, Johnson and Johnson, USA).
10
CRP Measurement Using Dry Chemistry
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C-reactive protein (CRP), an acute phase protein, was measured with the use of the dry chemistry immunological method and a VITROS 250 analyzer (Ortho Clinical Diagnostics, Johnson and Johnson, USA). Based on the hospital laboratory reference range, CRP concentration above 5 mg/L was considered abnormal.
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