Seven-week-old male C57BL/6 mice were obtained from Daehan Biolink, Co. Ltd. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specific pathogen-free conditions and a 12:12-h light-dark cycle (lights on at 08:00 and off at 20:00). The mice had ad libitum access to standard irradiated chow (Purina Mills, Seoul, Korea). The Animal Care and Use Committee of the Seoul Women’s University approved all experimental procedures involving animals.
Standard irradiated chow
Manufactured by Purina
Standard irradiated chow is a laboratory animal diet that has been exposed to ionizing radiation for the purpose of sterilization. This process eliminates microorganisms that could potentially contaminate the chow. The core function of standard irradiated chow is to provide a nutritionally-balanced diet for laboratory animals in a sterile format.
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Lab products found in correlation
7 protocols using standard irradiated chow
1
Specific Pathogen-Free C57BL/6 Mouse Housing
2
Animal Housing and Handling Protocol
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Seven-week-old C57BL/6 male mice were obtained from Daehan Biolink, Inc. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specific pathogen-free conditions with a 12:12-h (light-dark) cycle. All of the mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). All of the experimental procedures used in this study were approved by the Institutional Animal Care and Use Committee at Ewha Womans University, based on the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
3
Treadmill Running Protocols for Mice
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Seven-week-old male C57BL/6 mice were obtained from Daehan Biolink Co., Ltd. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specific pathogen-free conditions with a 12:12-h light-dark cycle (lights on at 08:00 and off at 20:00). Mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). All experimental procedures involving animals were approved by the Animal Care and Use Committee of Ewha Women’s University.
In this study, the exercise intensity protocol (high- and moderate-intensity) was modified according to a previous study [28 (link)], in which the intensity was set according to the VO-2max degree (moderate, > 50–60%; high, 75–85%). All mice were acclimated to treadmill running (Myung Jin Instruments Co., Seoul, Korea) by pre-exercise (Pre-Ex) at 5–8 m/min for 20 min/day for 5 days (each group N = 12). For the exercise regimen, the treadmill running speed was initially 10 m/min for 5 min and then increased as follows: moderate intensity 8–10 m/min for 20 min, high intensity 19–25 m/min for 20 min, and 5–8 m/min for 5 min. Finally, the speed was increased to 1 m/min weekly (moderate) and 2 m/min weekly (high).
In this study, the exercise intensity protocol (high- and moderate-intensity) was modified according to a previous study [28 (link)], in which the intensity was set according to the VO-2max degree (moderate, > 50–60%; high, 75–85%). All mice were acclimated to treadmill running (Myung Jin Instruments Co., Seoul, Korea) by pre-exercise (Pre-Ex) at 5–8 m/min for 20 min/day for 5 days (each group N = 12). For the exercise regimen, the treadmill running speed was initially 10 m/min for 5 min and then increased as follows: moderate intensity 8–10 m/min for 20 min, high intensity 19–25 m/min for 20 min, and 5–8 m/min for 5 min. Finally, the speed was increased to 1 m/min weekly (moderate) and 2 m/min weekly (high).
4
C57BL/6J Mice Housing and Care
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Seven weeks old male C57BL/6J mice were obtained from Daehan Biolink, Inc. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specified pathogen-free conditions and light-dark cycles of 12/12 hours (lights on at 0600 and off at 1800). All mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). All animal procedures were approved by the Animal Care and Use Committee of Dankook University, (Cheonan-si, Korea).
5
Murine Housing and Husbandry
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Seven week-old C57BL/6 male mice were obtained from Daehan Biolink, Inc (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specific pathogen-free conditions with a 12:12 h light-dark cycle (lights on at 6:00 and off at 18:00). Mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). Dankook University Animal Care and Use Committee granted approval for all experimental procedures involving animals.
6
C57BL/6J Mice Housing and Care
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7-week-old male C57BL/6J mice were obtained from Daehan Biolink, Inc. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specified pathogen-free conditions and light-dark cycles of 12/12 hours (lights on at 0600 and off at 1800). All mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). All animal procedures were approved by the Animal Care and Use Committee of Ewha Women’s University, (Seoul, Korea).
7
Murine Husbandry and Care
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Male 7-week-old C57BL/6 mice were obtained from Daehan Biolink, Co., Ltd. (Eumsung, Chungbuk, Korea) and housed in clear plastic cages under specific pathogen-free conditions and a 12:12-h light-dark cycle (lights on at 08:00 and off at 20:00). The mice had free access to standard irradiated chow (Purina Mills, Seoul, Korea). The Animal Care and Use Committee of Seoul Women’s University approved all experimental procedures involving animals.
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