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B6 cg tg tek cre 1ywa j

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The B6.Cg-Tg(Tek-cre)1Ywa/J is a transgenic mouse strain. It expresses Cre recombinase under the control of the Tek (Tie2) promoter.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (4 mentions) B6 cg gt rosa 26sortm14 cag tdtomato hze j tdtomato stock no, by Jackson ImmunoResearch (2 mentions) B6.129s7 ldlrtm1her j, by Jackson ImmunoResearch (1 mentions) Floxed trpa1, by Jackson ImmunoResearch (1 mentions) Fvb 129s6 b6 gt rosa 26sortm1 luc kael j, by Jackson ImmunoResearch (1 mentions) B6.129s7 asltm1brle j, by Jackson ImmunoResearch (1 mentions) B6.129p2 nos2tm1lau j, by Jackson ImmunoResearch (1 mentions)

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Tek-Cre (13 citations) Tie2-cre+/0(B6.Cg-Tg (Tek-cre)1Ywa/J) mice (2 citations)

13 protocols using b6 cg tg tek cre 1ywa j

1

Generation of Endothelial-Specific GSK3 Knockout Mice

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Cre-Lox technology was used to generate mice in which GSK3α or GSK3β is specifically ablated in ECs. LDLR−/− mice (B6.129S7-Ldlrtm1Her/J, Jackson Laboratory, Bar Harbor, ME) were crossed with mice carrying a loxP-flanked GSK3α (GSK3αfl/fl) gene or GSK3β floxed (GSK3βfl/fl) gene [37 (link),38 (link)] to create GSK3αfl/fl LDLR−/− or GSK3βfl/fl LDLR−/− mice. These mice were then crossed with a mouse that expressed Cre recombinase under the control of the endothelial specific Tie2 promoter (B6.Cg-Tg (Tek-Cre)1Ywa/J, Jackson Laboratories, Bar Harbor, ME) to create Tie2Cre GSK3αfl/fl LDLR−/− or Tie2Cre GSK3βfl/fl LDLR−/− mice. All mouse strains existed in a C57Bl/6 genetic background. Validation and characterization of these mice showed that the ablation of GSK3α and GSK3β occurred in ECs, as well as myeloid lineage cells (Supplementary Figure S1). Therefore, these mice were an endothelial/macrophage GSK3α or GSK3β knockout mouse model. To replenish GSK3α or GSK3β in the myeloid lineage, bone marrow from LDLR−/− mice was transplanted into GSK3αfl/fl LDLR−/−, GSK3βfl/fl LDLR−/−, Tie2Cre GSK3αfl/fl LDLR−/−, Tie2Cre GSK3βfl/fl LDLR−/−, or LDLR−/− (control) mice (see below). These mice were used as endothelial-selective GSK3α or GSK3β knockout mouse models.
Mastrogiacomo L, & Werstuck G.H. (2022). Investigating the Role of Endothelial Glycogen Synthase Kinase3α/β in Atherogenesis in Low Density Lipoprotein Receptor Knockout Mice. International Journal of Molecular Sciences, 23(23), 14780.
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2

Endothelial-Specific TRPA1 Knockout Mice

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eTRPA1−/− mice were created by crossing mice homozygous for loxP-flanked TRPA1 S5/S6 transmembrane domain construct (“floxed TRPA1”; The Jackson Laboratory, 008650 B.129-TRPA1tm2KyKw>/J) and mice hemizygous for Cre recombinase under the control of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer [The Jackson Laboratory, B6.Cg-Tg(Tek-cre)1Ywa/J]. Tek imparts consistent Cre expression exclusively in the endothelium during development and adulthood. F1 progeny positive for Cre were mated with homozygous floxed TRPA1 mice. F2 progeny were genotyped by PCR with genomic DNA obtained from ear or tail biopsies, and those positive for Cre and homozygous for floxed TRPA1 were crossed with homozygous floxed TRPA1 mice. eTRPA1−/− mice are viable and fertile. Control mice were homozygous for floxed TRPA1 but negative for Cre.
Sullivan M.N., Gonzales A.L., Pires P.W., Bruhl A., Leo M.D., Li W., Oulidi A., Boop F.A., Feng Y., Jaggar J.H., Welsh D.G, & Earley S. (2015). Localized TRPA1 channel Ca2+ signals stimulated by reactive oxygen species promote cerebral artery dilation. Science signaling, 8(358), ra2.
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3

Generation and Characterization of Acvr1R206H Mice

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Generation of Acvr1tnR206H mice was previously described (6 (link)), and standard breeding schemes were used to produce mice that carry the Tie2-Cre driver (17 (link)) [B6.Cg-Tg(Tek-cre)1Ywa/J; The Jackson Laboratory, 008863] and the reporters, R26NG (23 (link)) [derived from R26NZG; FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J; The Jackson Laboratory, 012429] or R26luc (22 (link)) [FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J; The Jackson Laboratory, 005125]. Adult mice between 8 and 16 weeks of age were used for all experiments. Only female mice were used in flow cytometry analysis of R206H-FAPs. Male and female mice were used interchangeably in all other studies. Mice were genotyped by PCR and reporter fluorescence, as previously described (6 (link), 12 (link)). All comparisons were made to littermate controls that lacked either the FOP allele or the Cre driver. These mice were phenotypically normal and are referred to as wild type throughout for simplicity.
Lees-Shepard J.B., Stoessel S.J., Chandler J.T., Bouchard K., Bento P., Apuzzo L.N., Devarakonda P.M., Hunter J.W, & Goldhamer D.J. (2022). An anti-ACVR1 antibody exacerbates heterotopic ossification by fibro-adipogenic progenitors in fibrodysplasia ossificans progressiva mice. The Journal of Clinical Investigation, 132(12), e153795.
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4

Lineage Tracing of Endothelial Cells

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C57BL6/J (WT), mT/mG reporter (Gt (ROSA)26Sortm14(CAG-tdTomato)Hze/J), and Tie2-cre (B6.Cg-Tg[Tek-cre]1Ywa/J) mice were purchased from Jackson Laboratories (JAX). Apelin (Apln)-creER mice were generated by Dr. Bin Zhou [18 (link), 22 (link), 23 (link)]. To generate lineage-tracing mice that specifically express endogenous fluorescent proteins in ECs, we crossed mT/mG mice with Tie2-cre or Apln-creER.
Jamaiyar A., Juguilon C., Wan W., Richardson D., Chinchilla S., Gadd J., Enrick M., Wang T., McCabe C., Wang Y., Kolz C., Clark A., Thodeti S., Ohanyan V., Dong F., Zhou B., Chilian W, & Yin L. (2022). The Essential Role for Endothelial Cell Sprouting in Coronary Collateral Growth. Journal of molecular and cellular cardiology, 165, 158-171.
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5

Endothelial-Specific DNA-PKcs Knockout Mice

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Homozygous DNA-PKcsf/f mice were generated as previously described 30 . Tie2Cre transgenic mice (B6.Cg-Tg(Tek-cre)1Ywa/J, stock no: 008863) were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). DNA-PKcsf/f mice were bred with Tie2Cre mice to generate EC-specific DNA-PKcs-knockout (DNA-PKcsf/f/Tie2Cre) mice. DNA-PKcsf/f mice were used as the control group for DNA-PKcsf/f/Tie2Cre mice. All mouse strains had a C57BL/6J background and were housed under specific pathogen-free conditions with a 12 h/12 h light/dark cycle under controlled temperature and humidity. Food and water were provided ad libitum.
Zou R., Shi W., Chang X., Zhang M., Tan S., Li R., Zhou H., Li Y., Wang G., Lv W, & Fan X. (2024). The DNA-dependent protein kinase catalytic subunit exacerbates endotoxemia-induced myocardial microvascular injury by disrupting the MOTS-c/JNK pathway and inducing profilin-mediated lamellipodia degradation. Theranostics, 14(4), 1561-1582.
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6

Transgenic Mouse Strains for Vascular Research

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C57BL/6J (wild-type; stock no. 000664), B6.Cg-Tg(Tek-cre)1Ywa/J (TIE2-cre; stock no. 008863), B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (tdTomato; stock no. 007914) mice were purchased from Jackson Laboratories (Bar Harbor, ME). B6.Cg-Tg(Tek-cre)1Ywa/J and B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J were crossed together to generate constitutive Tek-cre; tdTomato (TIE2-tdTomato) mice in our facilities. All mice were acclimated for 1 week prior to experiments and group housed (no more than 5 per cage) in pathogen-free conditions under a 14:10 hour light:dark cycle. Moreover, mice were housed at 20°C to 22°C with 30–70% humidity and fed ad libitum (Lab Diet 5001). To limit pathogen transmission, water was acidified to a pH of 2.5 to 3.0 with HCl for survival studies. All experiments were carried out using gender-matched littermate controls where appropriate. All mice in this study were used at 9–14 weeks of age. Both males and females were used for experiments.
Vercellino J., Małachowska B., Kulkarni S., Bell B.I., Shajahan S., Shinoda K., Eichenbaum G., Verma A.K., Ghosh S.P., Yang W.L., Frenette P.S, & Guha C. (2024). Thrombopoietin mimetic stimulates bone marrow vascular and stromal niches to mitigate acute radiation syndrome. Research Square.
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7

Generation of Cav1 Knockout Mice

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NR-specific Cav1 KO mice (Chx10-Cre; Cav1flox/flox) were generated using Cre-Lox technology.25 (link) Animals carrying Chx10-promoter-driven Cre recombinase (stock no. 005105; The Jackson Laboratory, Bar Harbor, ME, USA) were bred with animals carrying the Cav1 gene flanked by Lox P sites located upstream and downstream of exon 2.26 (link) Endothelial-specific Cav1 knock-out mice (Tie2-Cre; Cav1flox/flox) were generated similarly, using endothelial cell–specific recombination (B6.Cg-Tg (Tek-cre) 1Ywa/J; stock no. 008863; The Jackson Laboratory).27 (link) Cre-negative littermates were used as controls. Mice were screened for rd1 and rd8 mutations before this study. All procedures were approved by the University of Oklahoma Health Sciences Center Institutional Animal Care and Use Committee and comply with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Visual Research.
Gurley J.M., Gmyrek G.B., McClellan M.E., Hargis E.A., Hauck S.M., Dozmorov M.G., Wren J.D., Carr D.J, & Elliott M.H. (2020). Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina. Investigative Ophthalmology & Visual Science, 61(12), 19.
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8

Endothelial-Specific DLC1 Knockout Mice

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To produce endothelial-specific DLC1 knockout mouse, mice carrying the DLC1loxp/loxp allele established in our laboratory were crossbred with B6.Cg-Tg(Tek-cre1Ywa/J) from Jackson laboratory to generate DLC1-Tek mice. These mice were bred and maintained under specific pathogen-free conditions at the animal facility of University of California, Davis All the mice related manipulations were performed with protocols approved by the animal ethics committee at the University of California, Davis.
Shih Y.P., Yuan S.Y, & Lo S.H. (2017). Down-regulation of DLC1 in endothelial cells compromises the angiogenesis process. Cancer letters, 398, 46-51.
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9

Transgenic Mice for Lineage Tracing

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C57BL/6J (wild-type; stock no. 000664), B6.Cg-Tg (Tek-cre)1Ywa/J (TIE2-cre; stock no. 008863), B6.Cg-Gt (ROSA)26Sortm14 (CAG−tdTomato)Hze/J (tdTomato; stock no. 007914) mice were purchased from Jackson Laboratories (Bar Harbor, ME). B6.Cg-Tg (Tek-cre)1Ywa/J and B6.Cg-Gt (ROSA)26Sortm14 (CAG−tdTomato)Hze/J were crossed together to generate constitutive Tek-cre; tdTomato (TIE2-tdTomato) mice in our facilities. All mice were acclimated for 1 week prior to experiments and group housed (no more than 5 per cage) in pathogen-free conditions under a 14:10 h light:dark cycle. Moreover, mice were housed at 20 °C to 22 °C with 30–70% humidity and fed ad libitum (Lab Diet 5001). To limit pathogen transmission, water was acidified to a pH of 2.5 to 3.0 with HCl for survival studies. All experiments were carried out using gender-matched littermate controls where appropriate. All mice in this study were used at 9–14 weeks of age. Both males and females were used for experiments.
Vercellino J., Małachowska B., Kulkarni S., Bell B.I., Shajahan S., Shinoda K., Eichenbaum G., Verma A.K., Ghosh S.P., Yang W.L., Frenette P.S, & Guha C. (2024). Thrombopoietin mimetic stimulates bone marrow vascular and stromal niches to mitigate acute radiation syndrome. Stem Cell Research & Therapy, 15, 123.
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10

Selective Endothelial BBSome Disruption in Mice

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To selectively disrupt the BBSome in endothelial cells, we crossed Bbs1fl/fl female mice with Tie2Cre male mice, in which Cre recombinase is driven by a pan-endothelial-specific Tie2 promoter [18 (link)] (Strain: B6.Cg-Tg (Tek-cre)1Ywa/J; The Jackson Laboratory, stock no. 008863). To visualize Cre recombinase, we first crossed the Tie2Cre mice with the ROSA (tdTomato) reporter transgenic mouse line, in which a stop codon flanked by loxP sites precedes the start position of a tdTomato locus (Strain: ROSA [Stopfl/fl-tdTomato]; The Jackson Laboratory, stock no. 007914). Cre recombination removes the stop site, leading to the expression of the fluorescent tdTomato protein. All mice were maintained on the C57BL/6 J background for this study. The age at which mice were studied is indicated in the description of the results of each experiment.
Genotyping of the mice was performed using a polymerase chain reaction (PCR) assay, as described previously [4 (link)]. Animals were housed at the University of Iowa vivarium in a 23 °C temperature-controlled environment with a 12-h:12-h light–dark cycle (lights on: 6 AM–6 PM), with ad libitum access to tap water and either standard chow or a high-fat/high-sucrose (HFHS) diet (Research Diets Inc, D12331). Animal procedures were approved by the University of Iowa Institutional Animal Care and Use Committee.
Jiang J., Reho J.J., Bhattarai S., Cherascu I., Hedberg-Buenz A., Meyer K.J., Tayyari F., Rauckhorst A.J., Guo D.F., Morgan D.A., Taylor E.B., Anderson M.G., Drack A.V, & Rahmouni K. (2021). Endothelial BBSome is essential for vascular, metabolic, and retinal functions. Molecular Metabolism, 53, 101308.
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