Telaprevir (TPV) and staurosporine (STS) were obtained from AdooQ Biosciences and asunaprevir (ASV) was obtained by custom synthesis (Acme Bioscience). ASV is also commercially available as a research reagent. AP20187 (AP) was purchased from ApexBio. Stocks of each compound were made at 1000× of the final concentration in dimethysulfoxide (DMSO, Santa Cruz Biotechnology) and stored at −20 °C. For experiments with transfected plasmids, ASV and TPV were applied to cells directly prior to transfection, or along with transfection reagents, unless otherwise indicated. Cobalt(II) chloride hexahydrate (CoCl2, Sigma Aldrich) was dissolved in water to make a 400-mM (500×) stock. In applicable experiments, cells were incubated in 800 μM CoCl2 for the final 7 h before harvest, in order to simulate hypoxia.
Cobalt 2 chloride hexahydrate cocl2
Manufactured by Merck Group
Sourced in Germany
Cobalt(II) chloride hexahydrate (CoCl2•6H2O) is an inorganic compound that consists of cobalt (II) ions and chloride ions, with six water molecules attached. It is a crystalline solid that is typically blue in color. The core function of this compound is to serve as a source of cobalt ions for various chemical and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Santa Cruz Biotechnology (2 mentions)
Telaprevir tpv, by Adooq (2 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
Gsk2578215a, by Bio-Techne (1 mentions)
L glutamic acid monosodium salt hydrate, by Merck Group (1 mentions)
2 methoxyestradiol, by Merck Group (1 mentions)
Lrrk2 in 1, by Bio-Techne (1 mentions)
4 protocols using cobalt 2 chloride hexahydrate cocl2
1
Hypoxia Mimicry in Compound Treatments
2
Hypoxia Mimicry in Compound Treatments
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Telaprevir (TPV) and staurosporine (STS) were obtained from AdooQ Biosciences and asunaprevir (ASV) was obtained by custom synthesis (Acme Bioscience). ASV is also commercially available as a research reagent. AP20187 (AP) was purchased from ApexBio. Stocks of each compound were made at 1000× of the final concentration in dimethysulfoxide (DMSO, Santa Cruz Biotechnology) and stored at −20 °C. For experiments with transfected plasmids, ASV and TPV were applied to cells directly prior to transfection, or along with transfection reagents, unless otherwise indicated. Cobalt(II) chloride hexahydrate (CoCl2, Sigma Aldrich) was dissolved in water to make a 400-mM (500×) stock. In applicable experiments, cells were incubated in 800 μM CoCl2 for the final 7 h before harvest, in order to simulate hypoxia.
3
Hypoxia Induction in MSC Culture
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Hypoxia in MSC culture was induced by Cobalt (II) Chloride hexahydrate (CoCl2, Sigma–Aldrich, Darmstadt, Germany). Stock solution of 25 mM CoCl2 was prepared in sterile distilled water. MSCs were seeded at a density of 5 × 103 cells per well in 96-well culture plates for MTT proliferation assay and 105 cells per well on 6-well culture plate for analyses. After reaching ~80% confluency, culture media were replaced by adding new media containing 100 μM of CoCl2 and cells were incubated for 24 h. The cultured MSCs at passages 3–5 were used in all experiments.
4
Kinase Inhibitor Screening Protocol
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Cobalt(II) chloride hexahydrate (CoCl2), L-Glutamic acid monosodium salt hydrate, hydrogen peroxide (H2O2), and 2-Methoxyestradiol (2ME2) were purchased from Sigma-Aldrich. GSK2578215A (#4629) and LRRK2-IN-1 (#4273) were obtained from Tocris Bioscience. G1023, MLi-2, and PF-06447475 were synthesized by Dr. Hwan Geun Choi at Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF, Daegu, South Korea). Information about antibodies used in this study is provided in Supplementary Table 2 .
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