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Anti sox2

Manufactured by Active Motif
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Anti-SOX2 is an antibody product developed by Active Motif. It is designed to detect the expression of the SOX2 transcription factor, which plays a crucial role in the maintenance of stem cell pluripotency and the regulation of embryonic development.

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Lab products found in correlation

Anti nanog, by Active Motif (3 mentions) Vectashield mounting medium with dapi, by Vector Laboratories (2 mentions) Lsm 700 confocal microscope, by Zeiss (2 mentions) Anti ha 11, by Fortrea (2 mentions) Anti klf4, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Anti c myc, by Cell Signaling Technology (1 mentions) Anti oct4, by Cell Signaling Technology (1 mentions) Anti h4r3me2s, by Active Motif (1 mentions) Anti h3 antibody ab1791, by Abcam (1 mentions) Anti h3k36me2, by Active Motif (1 mentions)

4 protocols using anti sox2

1

Immunocytochemical Analysis of mESC Dux Expression

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mESC clones expressing an HA-tagged Dux protein were fixed for 20 min with 4% paraformaldhyde, permeabilized for 5 min with 0.1% Triton-X 100, and blocked for 30 min with 1% BSA in PBS. Cells were then incubated for 1 hour with anti-HA.11 (Covance) or anti-NANOG (Active Motif) or anti-SOX2 (Active Motif) antibodies diluted in PBS with 1% BSA. After 3 washes, the cells were incubated with anti-mouse (HA) or anti-rabbit (NANOG, SOX2) Alexa Fluor 647-conjugated secondary antibodies for 1 hour and washed again three times. Every step until this point, was carried with cells in suspension. Pelleted cells were then resuspended in VECTASHIELD® Mounting Medium with DAPI (Vector Laboratories) and mounted on the coverslip. The slides were viewed with a Zeiss LSM700 confocal microscope.
De Iaco A., Planet E., Coluccio A., Verp S., Duc J, & Trono D. (2017). A family of double-homeodomain transcription factors regulates zygotic genome activation in placental mammals. Nature genetics, 49(6), 941-945.
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2

Recombinant PRMT7 and Antibody Validation

Cited 1 time
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Anti-PRMT7 antibodies were purchased from Epicypher (#13-1009), Santa Cruz Biotechnology (SC9882) and Millipore (#07-639). Anti-Nanog (#61419), anti-Sox2 (#39823) and anti-H4R3me2s (#61187) antibodies were from Active Motif. Anti-Oct4 (#2840), anti-c-Myc (#5605) and anti-Klf4 (#4038) antibodies were from Cell Signaling. Anti-β-actin antibody (A5441) was from Sigma-Aldrich. Anti-H4R3me1 antibody (PA5-27065) was from Thermo Scientific. Anti-H3 antibody (ab1791) was from Abcam. Anti-mouse Argonaute 2 (Ago2) antibody was from Wako Chemicals (292-67301) and Cell Signaling (2897P). The cDNA constructs of human (OpenBiosystem) and mouse PRMT7 (OpenBiosystem) were cloned into pCDH1-EF1-IRES-GFP vector (System Biosciences) using the cloning sites EcoRI and NotI. Human PRMT7's catalytic mutant (m. PRMT7) was generated from pCDH1-EF1-PRMT7-IRES-GFP by mutating several residues (E144A, E153A, E478A and H644A) using the QuickChange Site-Directed Mutagenesis Kit (Stratagene). These residues are likely located in arginine binding pockets according to crystal structures of PRMT1 and Caenorhabditis elegans PRMT7 (18 (link),19 (link)). Oligonucleotides used for cloning, ChIP-PCR, reverse-transcription (RT)-PCR, site-directed mutagenesis are listed in Supplementary Table S1.
Lee S.H., Chen T.Y., Dhar S.S., Gu B., Chen K., Kim Y.Z., Li W, & Lee M.G. (2016). A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, Nanog, Klf4 and c-Myc to regulate stemness. Nucleic Acids Research, 44(22), 10603-10618.
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3

Immunocytochemical Analysis of mESC Dux Expression

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mESC clones expressing an HA-tagged Dux protein were fixed for 20 min with 4% paraformaldhyde, permeabilized for 5 min with 0.1% Triton-X 100, and blocked for 30 min with 1% BSA in PBS. Cells were then incubated for 1 hour with anti-HA.11 (Covance) or anti-NANOG (Active Motif) or anti-SOX2 (Active Motif) antibodies diluted in PBS with 1% BSA. After 3 washes, the cells were incubated with anti-mouse (HA) or anti-rabbit (NANOG, SOX2) Alexa Fluor 647-conjugated secondary antibodies for 1 hour and washed again three times. Every step until this point, was carried with cells in suspension. Pelleted cells were then resuspended in VECTASHIELD® Mounting Medium with DAPI (Vector Laboratories) and mounted on the coverslip. The slides were viewed with a Zeiss LSM700 confocal microscope.
De Iaco A., Planet E., Coluccio A., Verp S., Duc J, & Trono D. (2017). A family of double-homeodomain transcription factors regulates zygotic genome activation in placental mammals. Nature genetics, 49(6), 941-945.
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4

Western Blot Analysis of Cell Signaling

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Protein was extracted under the indicated conditions, and the protein lysate was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The proteins were then transferred to polyvinylidene fluoride membranes. After being blocked in 10% non-fat milk, the membranes were incubated with specific primary and secondary antibodies. The antibodies used were anti-SOX2 (Active Motif), anti-NSD2 (Cell Signaling Technology, CST), anti-H3K36me2 (Active Motif), anti-H3K27me3 (CST), anti-BCL2 (CST), anti-GAPDH (CST), anti-H3 (Abways), anti-ERK (CST), anti-pERK (CST), anti-AKT (CST) and anti-pAKT (CST).
He C., Liu C., Wang L., Sun Y., Jiang Y, & Hao Y. (2019). Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma. Cell Death & Disease, 10(2), 65.
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