Mccoy s 5a modified medium

Manufactured by Corning

McCoy's 5a Modified Medium is a cell culture medium formulated to support the growth and maintenance of various cell lines. It provides the necessary nutrients, salts, and other components required for optimal cell proliferation and viability. The medium is a modification of the original McCoy's 5a formulation, which is widely used in cell biology research and applications.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Corning (2 mentions) α minimum essential medium, by Thermo Fisher Scientific (1 mentions) Mc3t3 e1 subclone 4, by American Type Culture Collection (1 mentions) Rs2000 x ray irradiator, by Rad Source (1 mentions) Rpmi 1640, by Corning (1 mentions) U2os human osteosarcoma cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Hek293t cells, by Corning (1 mentions) Leibovitz s l 15 medium, by Corning (1 mentions) Hct116 cells, by Corning (1 mentions) Sw480, by Corning (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Vigofect, by Vigorous Biotechnology (1 mentions) Sw480 ccl 228, by American Type Culture Collection (1 mentions) Hek293t cells crl 3216, by American Type Culture Collection (1 mentions) Hct116 cells ccl 247, by American Type Culture Collection (1 mentions)

4 protocols using mccoy s 5a modified medium

Culturing Pre-Osteoblasts, U-2 OS, and HeLa

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Pre-osteoblast mouse cell line MC3T3-E1 subclone 4 (ATCC) was grown in alpha- Minimum Essential Medium (Gibco). U-2 OS and HeLa cells (ATCC) were grown in McCoy's 5a Modified Medium (Corning Cellgro) and DMEM, respectively. Cells were incubated at 37°C and 5% CO₂.

Almeida M.I., Silva A.M., Vasconcelos D.M., Almeida C.R., Caires H., Pinto M.T., Calin G.A., Santos S.G, & Barbosa M.A. (2015). miR-195 in human primary mesenchymal stromal/stem cells regulates proliferation, osteogenesis and paracrine effect on angiogenesis. Oncotarget, 7(1), 7-22.

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Osteosarcoma and Breast Cancer Cell Culture

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U2OS human osteosarcoma cells were purchased from the American Type Culture Collection. These cells were maintained in McCoy’s 5A modified medium (Cellgro, 10-050-CV) supplemented with 10% FBS. Breast cancer MDA-AB-231 and HS578T cells were maintained in RPMI 1640 (Corning,10-040-CV) and DMEM (Corning, 10-017-CV) supplemented with 10% FBS. Cells were incubated at 37 °C in a humidified incubator with 5% CO2. Cells were irradiated with a RAD SOURCE RS-2000 X-ray irradiator (Suwanee, GA) at the indicated doses.

Zhang L., Peng Y., Uray I.P., Shen J., Wang L., Peng X., Brown P.H., Tu W, & Peng G. (2017). Natural product β-thujaplicin inhibits homologous recombination repair and sensitizes cancer cells to radiation therapy. DNA repair, 60, 89-101.

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3D Colon Cancer Cell Proliferation

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In the study of cell proliferation and tumor growth in 3D, we chose a human colon cancer cell line HCT-116 (ATCC) as a cell model. Before loading the cells in the chip, we cultured them on tissue culture flasks in McCoy’s 5 A modified medium (Corning) with 10% (vol/vol) FBS (Gibco) and 1% penicillin/streptomycin (Gibco) at 37 °C and 5% CO₂ in a humidified incubator. The cell culture medium was changed every other day and passaged when cells reached over 80% confluency. When the cells were embedded in 3D collagen, we continued culturing them by submerging the chip in fresh cell culture medium. Passage numbers of the cells used in this research did not exceed ten.

Gong X, & Mills K.L. (2018). Large-scale patterning of single cells and cell clusters in hydrogels. Scientific Reports, 8, 3849.

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Mammalian Cell Culture and Plasmid Expression

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Human embryonic kidney HEK293T cells (CRL-3216) and human colorectal adenocarcinoma SW480 (CCL-228) and HCT116 cells (CCL-247) were obtained from American Type Culture Collection. HEK293T cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) at 37°C in a humidified, 5% CO₂ incubator. HCT116 cells were maintained in McCoy’s 5a Modified Medium (Corning) supplemented with 10% FBS, while SW480 cells were maintained in Leibovitz’s L-15 Medium (Corning) supplemented with 10% FBS without CO₂. Cell transfection was conducted with VigoFect (Vigorous Biotechnology) or Lipofectamine 2000 (Invitrogen).
All plasmids used in this study were generated by subcloning corresponding cDNAs into HA-pcDNA3.1 (for mammalian cell expression), pET32M.3C, pETMBP.3C (for bacteria expression), or pCS107-HA (for in vitro transcription) vectors. The sequences encoding human Axin1 (NCBI RefSeq no. NM_181050.3), human APC (NM_000038.6), human GSK3β (NM_001146156.2), β-catenin (NM_001098209.2), and human CK1α (NM_001025105.3) were amplified using standard PCR procedures with cDNA from HEK293T cells and subcloned into HA-pcDNA3.1, pETMBP.3C, and pCS107-HA vectors. Drosophila melanogaster dAPC2 (NM_001347814.1) was amplified and subcloned into pETMBP.3C vectors. Plasmids encoding Axin1 deletions or mutations were generated by PCR using primers with appropriate nucleotide substitutions.

Nong J., Kang K., Shi Q., Zhu X., Tao Q, & Chen Y.G. (2021). Phase separation of Axin organizes the β-catenin destruction complex. The Journal of Cell Biology, 220(4), e202012112.

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